Gold Nanoparticles Decorated with Sialic Acid Terminated Bi-antennary N-Glycans for the Detection of Influenza Virus at Nanomolar Concentrations

Poonthiyil, Vivek; Nagesh, Prashanth Thevkar; Husain, Matloob; Golovko, Vladimir B.; Fairbanks, Antony J.

doi:10.1002/open.201500109

Cited by 26 publications

(19 citation statements)

References 65 publications

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“…This effect was not observed with the small SAs from S. typhimurium that lack a CBM, highlighting the crucial role of the lectin domain in SA catalytic efficiency. On the other hand, previous reports described the efficiency of glycoclusters bearing multiple sialic acids or transition‐state sialosyl cation analogues for synergistic detection and inhibition of sialic acid binding proteins or viral neuraminidases, respectively.…”

Section: Introductionmentioning

confidence: 99%

Multivalent Thiosialosides and Their Synergistic Interaction with Pathogenic Sialidases

Brissonnet

assailly

Saumonneau

et al. 2019

Chemistry A European J

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Sialidases (SAs) hydrolyze sialyl residues from glycoconjugates of the eukaryotic cell surface and are virulence factors expressed by pathogenic bacteria, viruses, and parasites. The catalytic domains of SAs are often flanked with carbohydrate‐binding module(s) previously shown to bind sialosides and to enhance enzymatic catalytic efficiency. Herein, non‐hydrolyzable multivalent thiosialosides were designed as probes and inhibitors of V. cholerae, T. cruzi, and S. pneumoniae (NanA) sialidases. NanA was truncated from the catalytic and lectinic domains (NanA‐L and NanA‐C) to probe their respective roles upon interacting with sialylated surfaces and the synthetically designed di‐ and polymeric thiosialosides. The NanA‐L domain was shown to fully drive NanA binding, improving affinity for the thiosialylated surface and compounds by more than two orders of magnitude. Importantly, each thiosialoside grafted onto the polymer was also shown to reduce NanA and NanA‐C catalytic activity with efficiency that was 3000‐fold higher than that of the monovalent thiosialoside reference. These results extend the concept of multivalency for designing potent bacterial and parasitic sialidase inhibitors.

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Section: Introductionmentioning

confidence: 99%

Multivalent Thiosialosides and Their Synergistic Interaction with Pathogenic Sialidases

Brissonnet

assailly

Saumonneau

et al. 2019

Chemistry A European J

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“…Additionally, a convenient colorimetric assay has been developed using the interaction of the viral hemagglutinin protein with AuNPs modified with host glycan receptors. A key advantage of this assay is that the multivalency of glycan results in a strong avidity effect, which is known to improve weak protein‐glycan interactions (Figure 1 b) . This AuNP‐based assay improved the simplicity and sensitivity of glycan‐mediated biological detection systems.…”

Section: Solution‐based Virus Detectionmentioning

confidence: 97%

Metal Nanoparticles for Virus Detection

et al. 2016

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Viruses are small agents that can infect living creatures and cause harmful diseases. Rapid, sensitive virus detection would be beneficial for public health, and recent studies have shown that nanoparticles may have applications in virus detection. In particular, the unique properties of metal nanoparticles, originating from localized surface plasmons, allow for detection of virus through various methods. Additionally, the high surface‐to‐volume ratio and ease of surface modification of these nanoparticles provide advantages for bio‐applications. In this Focus Review, we describe currently available and recent advances in virus detection methods with metal nanoparticles. First, we outline several features of traditional virus detection methods and provide a brief explanation of metal nanoparticles. Then, we discuss solution‐based or substrate‐based virus detection according to the main operating phase of the detection elements. Finally, we evaluate the use of clinical samples for virus detection with metal nanoparticles.

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“…SNA forms strong bonds with AuNPs as compared to Saraca indica (saracin II), in the ground state as detected by UV-vis absorption, steady state, time-resolved fluorescence coupled with circular dichroism (CD) spectral studies, finding application in drug delivery systems [91]. AuNPs with sialic acid-terminated complex bi-antennary N-glycans, synthesized with glycans isolated from egg yolk, found application as sensor in detection of both recombinant HA and whole influenza A virus particles of the H1N1 subtype [92]. Aggregation of 4-mercaptophenylboronic acid functionalized AuNPs (4-MPBA-AuNPs) could bind to sialic acid and detected by colorimetric assays and finds importance in detection of sialic acid in blood serum samples [93].…”

Section: Nanotechnology Bioimaging Application Detection Of Cellularmentioning

confidence: 99%

Nanotechnology and sialic acid biology

Ghosh¹

2020

Sialic Acids and Sialoglycoconjugates in the Biology of Life, Health and Disease

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Gold Nanoparticles Decorated with Sialic Acid Terminated Bi-antennary N-Glycans for the Detection of Influenza Virus at Nanomolar Concentrations

Cited by 26 publications

References 65 publications

Multivalent Thiosialosides and Their Synergistic Interaction with Pathogenic Sialidases

Multivalent Thiosialosides and Their Synergistic Interaction with Pathogenic Sialidases

Metal Nanoparticles for Virus Detection

Nanotechnology and sialic acid biology

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