Gold nanoparticles based dipstick immunoassay for the rapid detection of dichlorodiphenyltrichloroethane: An organochlorine pesticide

Lísa, Miroslav; Chouhan, Raghuraj Singh; Vinayaka, Aaydha Chidambara; Manonmani, H. K.; Thakur, M. S.

doi:10.1016/j.bios.2009.05.006

Cited by 120 publications

(43 citation statements)

References 21 publications

(26 reference statements)

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“…Currently uni-molecular and array type of nano material based biosensors are being developed for detection of pesticides residues on consumables thereby indirectly improving the health system of man [38]. Interaction of the target with the biosensor can be measured either directly or indirectly by recording the changes in color, fluorescence or electrical potential.…”

Section: Detection Of Pesticides Using Nanobiosensorsmentioning

confidence: 99%

“…In array technologies, multiple biomolecules are fixed to a substrate allowing multiple analytes to be measured simultaneously. The gold NPs based dipstick technique was developed and found suitable for the detection of several toxins in food and environments and can also be applied for rapid on-site testing of pesticides [38].…”

Section: Detection Of Pesticides Using Nanobiosensorsmentioning

confidence: 99%

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The Role of Nanoscience and Technology in Medicine and Agriculture: Awareness Implication for Health Systems and Food Safety Issues

C¹

2017

JNMR

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The problems of diseases resistance to drugs in Medicine and low productivity in Agriculture have led to alternatives for improvements in health care systems and food safety issues. Nanotechnology is currently the chosen option for preparation and delivery of human drugs, pesticides and herbicides among other applications. Agriculture and medicine are competitive in sustenance of life without one substituting the other. This review paper therefore, examined some of the positive and negative effects of nanotechnology application in life and the awareness implication. Nanoparticles (NPs) system could be used for several routes of administration including oral, nasal, parenteral, intra-ocular benefits over free antimicrobial agents. The use of nanomaterials in the production of goods and drug formulations have shown superior properties compared with their conventional counterparts, due to their distinctive nanoscale features. Nanoparticles (NPs) have also consistently been reported to decrease infection, reduce scar tissue growth, and promote bone growth among other benefits. Nanotechnology may have concrete solutions against many agriculture-related problems like insect pest management, adverse effects of chemical pesticides, development of improved crop varieties. As with any other technology, controversy surrounding nanotechnology is no exception in a heterogeneous society. The toxic effects of antimicrobial NPs on central nervous system (CNS) are still unknown, interactions of NPs with the cells and tissues in CNS were poorly understood. Again, NPs represent size-specific properties that limit the use of currently available in vitro experiments in a general way, and there was no standardized definition for NP dose in mass, number, surface area, and biological samples (e.g., blood, urine, and inside organs that guarantee safety limit). Educating and training of consumers through seminars and workshops is recommended. National governments especially in developing countries are advised to mount regulating agencies that will be responsible for awareness creation and achieving optimum utilization of new products.

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Section: Detection Of Pesticides Using Nanobiosensorsmentioning

confidence: 99%

Section: Detection Of Pesticides Using Nanobiosensorsmentioning

confidence: 99%

The Role of Nanoscience and Technology in Medicine and Agriculture: Awareness Implication for Health Systems and Food Safety Issues

C¹

2017

JNMR

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“…It is highly stable in the environment and causes neurological changes and reproductive problems to wildlife. AuNPs based on a competitive immunoassay dip strip has been developed for the rapid testing of DDT (Lisa et al, 2009). The red AuNPs are conjugated to anti-DDT antibodies.…”

Section: Introductionmentioning

confidence: 99%

Development of ELISA kit for the assay of ichlorodiphenyltrichloroethane in milk and milk products

Kantharimuthu¹,

Balaraman²,

Sivanesan³

2016

Afr. J. Food Sci.

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Immunoassay of pesticide residues in foods is gaining importance in the past two decades. The objective of the present work is to develop an enzyme -linked immunosorbent assay (ELISA) kit for the assay of dichlorodiphenyltrichloroethane (DDT) in milk and milk products. Here, an ELISA method was developed using rabbit polyclonal antibodies against a hapten, DDT-OH for the detection of organochloride pesticide DDT in milk and milk products. The immunogen for raising the antiserum was prepared from BIS (4-chlorophenyl) ethanol and succinic anhydride coupled with bovine serum albumin (BSA) One ml of immunogen was inoculated subcutaneously in multiple sites of thigh region in rabbits following three boosters at weekly intervals and the antiserum was harvested after the second booster. The ELISA response of the anti DDT antiserum, diluted and undiluted was studied against the antigen (DDT) coated onto the immuno module. The homogenous response of the anti DDT anti serum was adequate to identify DDT. Fifteen samples each of milk, buttermilk, cheese, kalakhand and khoa were quantitatively analyzed for DDT using ELISA as well as GC methods. DDT was present only below detectable limit (BDL) (5 ng g -1 ) in milk and milk products obtained from organized dairies in and around Chennai city, India. The percentage recovery was above 70% ensuring that the ELISA method developed for DDT is dependable. The developed ELISA method can be helpful in detecting the residual amount of DDT present in milk and milk products. The analysis of pesticides in samples by ELISA and GC was comparable.

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“…23,24 The unique optical and electronic properties of gold nanoparticles make them ideal candidates for cell targeting and also as efficient tools for bioassays. 25 On this basis, we sought to develop a fluorescence-based biosensor using liver cancer cell surface-specific (LCCS) antibody-conjugated gold colloids to probe cancer cells specifically in a mixed cell population.…”

Section: Introductionmentioning

confidence: 99%

Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer

Chauhan

Zubair²,

Tufail³

et al. 2011

IJN

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Background: Nanomaterials are considered to be the pre-eminent component of the rapidly advancing field of nanotechnology. However, developments in the biologically inspired synthesis of nanoparticles are still in their infancy and consequently attracting the attention of material scientists throughout the world. Keeping in mind the fact that microorganism-assisted synthesis of nanoparticles is a safe and economically viable prospect, in the current study we report Candida albicans-mediated biological synthesis of gold nanoparticles. Methods and results: Transmission electron microscopy, atomic force microscopy, and various spectrophotometric analyses were performed to characterize the gold nanoparticles. The morphology of the synthesized gold particles depended on the abundance of C. albicans cytosolic extract. Transmission electron microscopy, nanophox particle analysis, and atomic force microscopy revealed the size of spherical gold nanoparticles to be in the range of 20-40 nm and nonspherical gold particles were found to be 60-80 nm. We also evaluated the potential of biogenic gold nanoparticles to probe liver cancer cells by conjugating them with liver cancer cell surface-specific antibodies. The antibody-conjugated gold particles were found to bind specifically to the surface antigens of the cancer cells. Conclusion:The antibody-conjugated gold particles synthesized in this study could successfully differentiate normal cell populations from cancerous cells.

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Gold nanoparticles based dipstick immunoassay for the rapid detection of dichlorodiphenyltrichloroethane: An organochlorine pesticide

Cited by 120 publications

References 21 publications

The Role of Nanoscience and Technology in Medicine and Agriculture: Awareness Implication for Health Systems and Food Safety Issues

The Role of Nanoscience and Technology in Medicine and Agriculture: Awareness Implication for Health Systems and Food Safety Issues

Development of ELISA kit for the assay of ichlorodiphenyltrichloroethane in milk and milk products

Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer

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