Gold nanoparticles and polyethylene glycols functionalized conducting polyaniline nanowires for ultrasensitive and low fouling immunosensing of alpha-fetoprotein

Hui, Ni; Sun, Xiaotian; Song, Zhiling; Niu, Shuyan; Liu, Xiang

doi:10.1016/j.bios.2016.06.028

Cited by 64 publications

(15 citation statements)

References 44 publications

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“…Conducting polymers, owing to their unique physical and chemical properties, have been widely used as substrates for the construction of electrochemical biosensors. Among these, the conjugated π-electron backbone of polyaniline (PANI) supports very useful characteristics, including a reversibility in redox, high electronic conductivity, and good biocompatibility . Additionally, its amino groups are amenable to subsequent (bio)functionalization .…”

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confidence: 99%

Electrochemical Aptasensor for Ultralow Fouling Cancer Cell Quantification in Complex Biological Media Based on Designed Branched Peptides

et al. 2019

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The rapid, convenient, and selective assaying of clinical targets directly in complex biological media brings with it the potential to revolutionize diagnostics. One major hurdle to impact is retention of selectivity and a tight control of nonspecific surface interactions or biofouling. We report herein, the construction of an antifouling interface through the covalent attachment of designed branched zwitterionic peptides onto electrodeposited polyaniline film. The antifouling capability of the designed branched peptide significantly outperforms that of the commonly used PEG and linear peptides. The interfaces modified with branched peptides are exceptionally effective in reducing a nonspecific protein and cell adsorption, as verified by electrochemical and fluorescent characterization. The derived sensors with mucin1 protein (MUC1) aptamer as the recognition element detect MUC1-positive MCF-7 breast cancer cells in human serum with high sensitivity and selectivity. The linear response range of the cytosensor for the MCF-7 cell is from 50 to 106 cells/mL, with a limit of detection as low as 20 cells/mL. More importantly, the assaying performances remain unchanged in human serum owing to the presence of branched antifouling peptide, indicating feasibility of the cytosensor for practical cancer cell quantification in complex samples.

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confidence: 99%

Electrochemical Aptasensor for Ultralow Fouling Cancer Cell Quantification in Complex Biological Media Based on Designed Branched Peptides

et al. 2019

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“…The circle section of the Nyquist plot at higher frequencies corresponds to the electron transfer of [Fe(CN) 6 ] 3−/4− . The difference in semicircular diameter or Rct indicates that SPCE has been successfully modified, the higher the concentration of biomolecules, the higher the Rct, inversely proportional to the current in the electron transfer process [33]. In figure 3d, spectrum a shows a large Rct for the SPCE before modification.…”

Section: Screen-printed Carbon Electrode Modification With Electrodeposition Of Cerium Oxidementioning

confidence: 98%

An aptasensor using ceria electrodeposited-screen-printed carbon electrode for detection of epithelial sodium channel protein as a hypertension biomarker

et al. 2021

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Epithelial sodium channel (ENaC) is a transmembrane protein that has an essential role in maintaining the levels of sodium in blood plasma. A person with a family history of hypertension has a high enough amount of ENaC protein in the kidneys or other organs, so that the ENaC protein acts as a marker that a person is susceptible to hypertension. An aptasensor involves aptamers, which are oligonucleotides that function similar to antibodies, as sensing elements. An electrochemical aptasensor for the detection of ENaC was developed using a screen-printed carbon electrode (SPCE) which was modified by electrodeposition of cerium oxide (CeO 2 ). The aptamer immobilization was via the streptavidin–biotin system. The measurement of changes in current of the active redox [Fe(CN) 6 ] 3−/4− was carried out by differential pulse voltammetry. The surfaces of SPCE and SPCE/CeO 2 were characterized using scanning electron microscopy, voltammetry and electrochemical impedance spectroscopy. The Box–Behnken experimental optimization design revealed the streptavidin incubation time, aptamer incubation time and streptavidin concentrations were 30 min, 30 min and 10.8 µg ml −1 , respectively. Various concentrations of ENaC were used to obtain the linearity range of 0.05–3.0 ng ml −1 , and the limits of detection and quantification were 0.012 ng ml −1 and 0.038 ng ml −1 , respectively. This aptasensor method has the potential to measure the ENaC protein levels in urine samples as well as to be a point-of-care device.

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“…Hui et al proposed electrochemically- synthesized polyaniline (PANI) nanowire for the measurement of alpha-fetoprotein, a biomarker of hepatocellular carcinoma [ 48 ]. PANI has usually been employed as an electron mediator to transport electrical signals toward the electrode, which can be facilitated by gold nanoparticles and enables not only conductance in neutral pH environments [ 48 , 49 ] but also increased charge transfer and PANI-based matrix stability [ 33 , 50 , 51 , 52 ]. Polyethylene glycol (PEG) was also employed as an additive to provide the electrochemical immunosensor with anti-fouling capabilities [ 48 ].…”

Section: Functional Nanomaterials Used As Electrodes and Supportinmentioning

confidence: 99%

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

Cho

Lee

Kim

et al. 2018

Sensors

182

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An electrochemical immunosensor employs antibodies as capture and detection means to produce electrical charges for the quantitative analysis of target molecules. This sensor type can be utilized as a miniaturized device for the detection of point-of-care testing (POCT). Achieving high-performance analysis regarding sensitivity has been one of the key issues with developing this type of biosensor system. Many modern nanotechnology efforts allowed for the development of innovative electrochemical biosensors with high sensitivity by employing various nanomaterials that facilitate the electron transfer and carrying capacity of signal tracers in combination with surface modification and bioconjugation techniques. In this review, we introduce novel nanomaterials (e.g., carbon nanotube, graphene, indium tin oxide, nanowire and metallic nanoparticles) in order to construct a high-performance electrode. Also, we describe how to increase the number of signal tracers by employing nanomaterials as carriers and making the polymeric enzyme complex associated with redox cycling for signal amplification. The pros and cons of each method are considered throughout this review. We expect that these reviewed strategies for signal enhancement will be applied to the next versions of lateral-flow paper chromatography and microfluidic immunosensor, which are considered the most practical POCT biosensor platforms.

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Gold nanoparticles and polyethylene glycols functionalized conducting polyaniline nanowires for ultrasensitive and low fouling immunosensing of alpha-fetoprotein

Cited by 64 publications

References 44 publications

Electrochemical Aptasensor for Ultralow Fouling Cancer Cell Quantification in Complex Biological Media Based on Designed Branched Peptides

Electrochemical Aptasensor for Ultralow Fouling Cancer Cell Quantification in Complex Biological Media Based on Designed Branched Peptides

An aptasensor using ceria electrodeposited-screen-printed carbon electrode for detection of epithelial sodium channel protein as a hypertension biomarker

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

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