Gold Nanoparticle Size and Shape Effects on Cellular Uptake and Intracellular Distribution of siRNA Nanoconstructs

Yue, Jun; Feliciano, Timothy; Li, Wenlong; Lee, Andrew; Odom, Teri W.

doi:10.1021/acs.bioconjchem.7b00252

Cited by 133 publications

(131 citation statements)

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“…10). 387 As mentioned in an earlier section of this review, the aggregation of NPs can change their physical properties. Therefore, TEM has been applied to characterize the dispersion of NPs after their internalization.…”

Section: Microscopy Techniques For Np Characterizationmentioning

confidence: 91%

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

2018

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Nanostructures have attracted huge interest as a rapidly growing class of materials for many applications. Several techniques have been used to characterize the size, crystal structure, elemental composition and a variety of other physical properties of nanoparticles. In several cases, there are physical properties that can be evaluated by more than one technique. Different strengths and limitations of each technique complicate the choice of the most suitable method, while often a combinatorial characterization approach is needed. In addition, given that the significance of nanoparticles in basic research and applications is constantly increasing, it is necessary that researchers from separate fields overcome the challenges in the reproducible and reliable characterization of nanomaterials, after their synthesis and further process (e.g. annealing) stages. The principal objective of this review is to summarize the present knowledge on the use, advances, advantages and weaknesses of a large number of experimental techniques that are available for the characterization of nanoparticles. Different characterization techniques are classified according to the concept/group of the technique used, the information they can provide, or the materials that they are destined for. We describe the main characteristics of the techniques and their operation principles and we give various examples of their use, presenting them in a comparative mode, when possible, in relation to the property studied in each case.

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Section: Microscopy Techniques For Np Characterizationmentioning

confidence: 91%

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

2018

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“…Next, we examined the interaction of unmodified polymersomes, ssDNA-polymersomes and the small and large clusters with U87-MG glioblastoma cells, known to have a higher endocytotic potential and favor uptake of larger sized particles. 42,43 Interestingly, we observed limited internalization of both the 400 nm clusters and to a lesser extent of the larger clusters (Fig. 2D, Fig.…”

Section: Examination Of Dna-linked Polymersome Clusters' Behavior In mentioning

confidence: 72%

DNA-directed arrangement of soft synthetic compartments and their behavior in vitro and in vivo

et al. 2020

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“…Gold nanomaterials are also accessible in many other geometries. For example, Yue et al investigated the uptake of gold nanostars (≈40 nm) in comparison to differently sized spherical particles (diameters ≈13 and 50 nm) into U87 glioblastoma cells . These studies indicated that the star shape results in an enhanced liberation of the nanostars from intracellular vesicles, which can be explained by membrane rupture processes initiated by the star spikes.…”

Section: Particle Properties and Resulting Implications On Particle–cmentioning

confidence: 99%

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

2018

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between individual uptake mechanisms for respective nanoparticle systems. Cells internalizing nanoparticles respond to the nanoparticles and different intracellular processes will be initiated, possibly resulting in the impairment of the cellular key functions. [4] Even though these investigations provide data with statistical relevance, they can merely provide an overall picture of the uptake mechanism, as issues of different uptake mechanisms in anisotropic nanoparticle suspensions are barely assessable. Simultaneously, the influence of the biological environment, e.g., the presence of serum proteins, salts, or altered pH conditions, may have a severe impact on the particle properties, which can entail various biological implications. As such, the role of high resolution imaging techniques, which provide a clear evidence for the nanoparticles' fate in the cellular environment, [5] their localization and targeting to different cellular organelles, as well as evidence for uptake and release mechanisms, have gained increasing importance. In particular, transmission electron microscopy (TEM) investigations provide attractive possibilities to study the interaction of particles with biological barriers, i.e., the extracellular membrane or the nuclear membrane, their accumulation into cellular organelles or their spatial distribution on the scale of individual nanoparticles [6] (e.g., uptake as individual nanoparticles or in form of large clusters, etc.). In addition TEM can simultaneously image also the cellular membranes and organelles to determine respective morphological changes [7] upon nanoparticle interaction. [8][9][10][11] Thus, attractive possibilities to acquire additional information on the particle fate inside the cell emerge from TEM investigations.It can be stated here, that the utilization of electron microscopy has been conducted during the last decades [5] and, in view of some extent electron microscopy investigations of particle uptake, can be regarded in this sense as a routinely applied technique. However, frequently TEM is only utilized to characterize the nanoparticle system or to prove particle internalization. Only a fraction of reported studies utilize TEM analysis to reveal details of nanoparticle uptake and internalization or even deduce uptake pathways from these studies. This might be related to the fact that sometimes the interpretation of TEM data is not straightforward but a number of experimental limitations have to be taken into account for such studies. In a recent review, the advantages and disadvantages of TEM investigations in the context of the The internalization of nanoparticles into mammalian cells relies on a complex interplay of several parameters, which enable and dictate uptake and fate of nanoparticles in the cellular system. Due to the complexity of the involved processes, a careful experimental design has to be developed to elucidate peculiarities of the uptake processes of new nanoparticle systems into cells. Individual parameters can be hardly considered alone but h...

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Gold Nanoparticle Size and Shape Effects on Cellular Uptake and Intracellular Distribution of siRNA Nanoconstructs

Cited by 133 publications

References 48 publications

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

DNA-directed arrangement of soft synthetic compartments and their behavior in vitro and in vivo

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

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