Gold Nanoparticle Probe-Assisted Antigen-Counting Chip Using SEM

Zhou, Xin; Yang, Chih‐Tsung; Xu, Qiaoshu; Lou, Zhichao; Xu, Zhengfeng; Thierry, Benjamin; Gu, Ning

doi:10.1021/acsami.8b19055

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…The detection limit was as low as 7.6 pg mL −1 (3 σ/K , where σ stands for the standard deviation of 10 blank controls, K stands for the slope of regression equation). The analytical performances of other CEA assays [ 22 , 23 , 24 , 25 , 26 , 27 , 28 ] were listed in Table S1 and compared with our designed ELISA method. The results showed that the sensitivity of this FLISA method was comparable or even better than other analytical methods.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

et al. 2022

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Copper peroxide/zeolitic imidazolate framework/polydopamine nanoparticles (CP/ZIF-8/PDA)-based fluorescence-linked immunosorbent assay (FLISA) was designed for the sensitive and high-throughput determination of carcinoembryonic antigen (CEA) by self-supplied H2O2 generation. Specifically, the CEA aptamer was modified on the surface of CP/ZIF-8/PDA to form an immunoprobe. The structures of CP and ZIF-8 could be broken under acidic conditions, and produced the Cu2+ and H2O2 due to the dissociation the CP. A subsequent Fenton-type reaction of Cu2+ and H2O2 generated hydroxyl radical (·OH). o-phenylenediamine (OPD) was oxidized by the ·OH to form 2, 3-diaminophenazine (DPA) with a significant fluorescence signal. CP/ZIF-8/PDA could be used as an efficient Fenton-type reactant to generate a large amount of ·OH to promote OPD oxidation. The sensitive detection of CEA could be realized. Under optimal conditions, the FLISA platform displayed a linear detection range from 0.01 to 20 ng mL−1 with a detection limit of 7.6 pg mL−1 for CEA. This strategy has great application potential for sensitive and high-throughput determination for other biomarkers in the field of biomedicine.

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Section: Resultsmentioning

confidence: 99%

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

et al. 2022

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“…Third, most of the current protein microarray results are difficult to quantify accurately. To achieve accurate quantification, which is key to most systems biology research, some researchers have attempted to improve the protein microarray quantitation methods, such as the gold nanoparticle (GNP) probe-assisted sandwich-counting strategy proposed by Zhou et al [108].…”

Section: Perspectivesmentioning

confidence: 99%

Research progress in protein microarrays: Focussing on cancer research

Chen

Yang

Liu

et al. 2022

Proteomics Clinical Apps

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Although several effective treatment modalities have been developed for cancers, the morbidity and mortality associated with cancer continues to increase every year. As one of the most exciting emerging technologies, protein microarrays represent a powerful tool in the field of cancer research because of their advantages such as high throughput, small sample usage, more flexibility, high sensitivity and direct readout of results. In this review, we focus on the research progress in four types of protein microarrays (proteome microarray, antibody microarray, lectin microarray and reversed protein array) with emphasis on their application in cancer research. Finally, we discuss the current challenges faced by protein microarrays and directions for future developments. We firmly believe that this novel systems biology research tool holds immense potential in cancer research and will become an irreplaceable tool in this field.

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“…Plasmonic metal nanoparticle (MNP) based assays have been applied to detecting multiple species of analytes from smallest molecules to large cells through a variety of signal transduction mechanisms, e.g., surface plasmon resonance, surface enhance Raman spectra, AuNP enhanced fluorescent, colorimetry, scanning electron microscope (SEM) and so on [24][25][26][27][28] . Here in our Nano2RED assay design, AuNPs are conjugated with synthesized nanobodies to form multivalent sensors that aggregate at the presence of specific antigen and subsequently precipitate, thus producing antigen-concentration-dependent signals that can be generated within minutes and 7 readily quantified by optical and electronic readout.…”

Section: Nanobody-conjugated Nanoparticles For Rapid Electronic Detection (Nano2red)mentioning

confidence: 99%

Synthetic Nanobody-Functionalized Nanoparticles for Accelerated Development of Rapid, Accessible Detection of Viral Antigens

Chen

Kang

Ikbal

et al. 2021

Preprint

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The success of controlling emerging infectious diseases relies on the fast development of robust, quantitative assays for point-of-care testing. Here a generalizable strategy is demonstrated for developing inexpensive, simple-to-use, and rapid diagnostics within a few weeks upon the identification of a new viral antigen. Using Ebola virus secreted glycoprotein (sGP) as a target, we design a new assay featuring nanobody-conjugated nanoparticles for rapid, electronic detection (Nano2RED). Nanobodies with the high affinity and specificity were generated by phage display screening of a high-quality combinatorial library (> 109) and site-specifically conjugated to gold nanoparticles (AuNPs) for in-solution colorimetric detection. Our assay can robustly detect the sGP protein from 10 pM to 100 nM in diluted serum and distinguish it from a membrane-anchored isoform, GP1,2, allowing the diagnosis of the viral infection stage. Additionally, a rapid assay protocol was established to decrease the assay time to a few minutes without compromising the accuracy. Lastly, this assay has been integrated with a portable semiconductor device with a digital readout and minimal training requirement for end users. Our method can be widely applied to the point-of-care testing of other infectious diseases.

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Gold Nanoparticle Probe-Assisted Antigen-Counting Chip Using SEM

Cited by 12 publications

References 33 publications

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

Research progress in protein microarrays: Focussing on cancer research

Synthetic Nanobody-Functionalized Nanoparticles for Accelerated Development of Rapid, Accessible Detection of Viral Antigens

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