Gold nanoparticle-enhanced target for MS analysis and imaging of harmful compounds in plant, animal tissue and on fingerprint

Mass Spectrometry Imaging of low Molecular Weight Compounds in Garlic (Allium sativumL.) with Gold Nanoparticle Enhanced Target

“…Low molecular weight metabolites, such as amino acids, small organic acids, flavonoid and flavonoid glycosides, benzoxazinoids and sulpholipids, have been already imaged in corn leaf (Korte and Lee, ; Korte et al , ). Distributions of metabolites within onion ( Allium cepa ) have been studied by Shrestha et al () with the use of laser ablation electrospray ionisation (LAESI) and also by our group (Sekuła et al , ).…”

On-Tissue Derivatization via Electrospray Deposition for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Endogenous Fatty Acids in Rat Brain Tissues

“…However, because of their low molecular weight (200–400 Da), low abundance in tissue, and poor ionization efficiency, direct MALDI MSI of these molecules in tissues using traditional matrixes such as 2,5-dihydroxy benzoic acid (DHB) or 9-aminoacridine (9-AA) has been challenging. A variety of low-background MALDI MS matrixes, e.g., silicon nanoparticles, 17 graphite, 18 pencil lead, 19 powdered carbon aerogels, 20 silver/gold nanoparticles, 21 , 22 and graphene, 23 have been introduced and applied to fatty acid detection, resulting in high coverage and sensitivity for FFAs. 18 , 20 , 23 However, these techniques may restrict detection of other classes of analytes compared to traditional matrixes, 24 frequently due to their affinity for specific classes of molecules 21 , 22 , 25 or fragmentation of higher molecular weight analytes.…”

“… 26 , 27 Moreover, few of these approaches have been applied to the characterization of FFAs by MSI. 21 , 22 Time-of-flight (TOF)-SIMS is commonly utilized for FFA imaging in tissue sections but typically fragments large molecular ions. 28 , 29 For MALDI-based imaging, high-mass resolution instruments such as Fourier transform ion cyclotron resonance (FTICR) 30 , 31 can discriminate FFAs from MALDI matrix interference in brain tissue sections.…”

Mass Spectrometry Imaging: A Review of Emerging Advancements and Future Insights

“…3,91,236 Furthermore, the use of plants as an alternative research system is commonplace so, naturally, researchers have also transitioned to imaging plant-based samples. 234,242 In the last 2 years, food products, such as onions, 243 cucumbers, 18 and citrus peel extracts, 237 have been analyzed, but it is clear that theoretically any item can be imaged with the appropriate equipment. 244,245 For example, DESI-MSI was utilized to understand the thin-layer chromatography (TLC) separation of the citrus peel extracts directly on the TLC plates (Figure 8).…”