Gold nanoparticle enhanced fluorescence anisotropy for the assay of single nucleotide polymorphisms (SNPs) based on toehold-mediated strand-displacement reaction

Wang, Xinyi; Zou, Mingjian; Huang, Huei-Ping; Ren, Yan; Li, Limei; Yang, Xiaoda; Li, Na

doi:10.1016/j.bios.2012.09.023

Cited by 65 publications

(35 citation statements)

References 46 publications

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“…The method consists in designing probes that identify complementary and contiguous sequences on the target, where hybridization forces the dye into close vicinity of the AuNPs' surface and the fluorescence signal decreases [66]. More recently, Wang et al proved that the fluorescence quenching/enhancement conferred by AuNP could be used as an SNP genotyping system suitable for point of care [67].…”

Section: Gold Nanoparticles Modulation Of Fluorescencementioning

confidence: 99%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their molecular characterization, genomic sequences associated with medical conditions (including cancer), mutation and polymorphism identification, and the quantification of gene expression.

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Section: Gold Nanoparticles Modulation Of Fluorescencementioning

confidence: 99%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

View full text Add to dashboard Cite

show abstract

“…For example, enzyme conjugates (Patolsky et al, 2001;Ermini et al, 2014) and nanoparticles (Abbaspour and Noori, 2012;Wang et al, 2013) have been used as amplifying labels for SNP genotyping. In addition, as the standard method for amplification of nucleic acids, polymerase chain reaction (PCR) is used to detect trace amount of samples from genomic DNA, which however could introduce errors into SNP detection during the exponential amplification process (Mhlanga and Malmberg, 2001).…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence resonance energy transfer imaging on magnetic particles for single-nucleotide polymorphism detection based on ligation chain reaction

Zhang

Dong

et al. 2015

Biosensors and Bioelectronics

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“…sequences [17,18]. The reporter probe cannot effectively release 128 from the pre-hybridized strand.…”

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confidence: 99%

“…Toehold-SDR can take place without an enzyme at room 28 temperature, and the kinetic rate can be controlled by adjusting 29 the length and sequence composition of the toehold, which is used 30 widely in analyzing SNPs as its enzyme-free, robustness, specificity 31 and isothermality [16][17][18]. Li et al combined gold nanoparticle 32 with fluorescence anisotropy for the assay of SNP with the 33 detection limit of 0.95 nmol/L based on toehold-mediated SDR [17] 34 and Wang et al combined quartz crystal microbalance and 35 toehold-SDR for the detection of SNP with 0.3 nmol/L limit [18]. in PBS solution as described before [19].…”

mentioning

confidence: 99%