Gold Nanoparticle-Based Quantitative Electrochemical Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband Electrodes

Authier, Laurent; Grossiord, Céline; Brossier, Pierre

doi:10.1021/ac0103221

Cited by 320 publications

(232 citation statements)

References 45 publications

Supporting

Mentioning

226

Contrasting

Unclassified

Order By: Relevance

“…Only a few authors have reported the coupling of biosensors with a DNA amplification method (e.g. PCR) to obtain reliable measurements of clinical interest (Marrazza et al, 2000;Azek et al, 2000;Authier et al, 2001;Meric et al, 2002;Lucarelli et al, 2002). Direct, enzyme-based, detection of DNA or RNA with no PCR pre-amplification has rarely been reported (Downs et al, 1988;Wojciechowski et al, 1999).…”

Section: Hybridisation With the Target Sequencementioning

confidence: 99%

“…In order to prevent the non-specific adsorption of unwanted solution constituents onto transducer surfaces, the use of blocking agents has been also proposed (de Lumley-Woodyear et al, 1996;Authier et al, 2001).…”

Section: Hybridisation With the Target Sequencementioning

confidence: 99%

“…Gold nanoparticles. Brossier's group reported the use of thiol-modified DNA probes anchored (by chemisorption) to gold nanoparticles (Authier et al, 2001). The method was applied for the sensitive quantification of an amplified 406 bp DNA sequence related to human cytomegalovirus.…”

Section: Direct Covalent Labelling Of Dna Target Sequencementioning

confidence: 99%

See 2 more Smart Citations

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Lucarelli

Marrazza

Turner

et al. 2004

Biosensors and Bioelectronics

374

204

View full text Add to dashboard Cite

Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples.

show abstract

Section: Hybridisation With the Target Sequencementioning

confidence: 99%

Section: Hybridisation With the Target Sequencementioning

confidence: 99%

See 1 more Smart Citation

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Lucarelli

Marrazza

Turner

et al. 2004

Biosensors and Bioelectronics

374

204

View full text Add to dashboard Cite

show abstract

“…When employed as signal-generating labels in the detection of biological affinity reactions such as DNA hybridization and immunoassays, gold and semiconductor nanoparticles have improved the detection sensitivity by orders of magnitude over their molecular counterparts [7][8][9][10][11][12][13]. This is attributed to the fact that each biological binding event is tagged with at least one * Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Enhanced electrochemical activity of redox-labels in multi-layered protein films on indium tin oxide nanoparticle-based electrode

Yang

Guo

2009

Analytica Chimica Acta

View full text Add to dashboard Cite

a b s t r a c tFacile electrical communication between redox-active labeling molecules and electrode is essential in the electrochemical detection of bio-affinity reactions. In this report, nanometer-sized indium tin oxide (ITO) particles were employed in the fabrication of porous thick film electrodes to enhance the otherwise impeded electrochemical activity of redox labels in multi-layered protein films, and to enable quantitative detection of avidin/biotin binding interaction. To carry out the affinity reaction, avidin immobilized on an ITO electrode was reacted with mouse IgG labeled with both biotin and ruthenium Tris-(2,2 -bipyridine) (Ru-bipy). The binding reaction between avidin and biotin was detected by the catalytic voltammetry of Ru-bipy in an oxalate-containing electrolyte. On sputtered ITO thin film electrode, although a single layer of Ru-bipy labeled avidin exhibited substantial anodic current, attaching the label to the outer IgG layer of the avidin/biotin-IgG binding pair resulted in almost complete loss of the signal. However, electrochemical current was recovered on ITO film electrodes prepared from nanometer-sized particles. The surface of the nanoparticle structured electrode was found by scanning electron microscopy to be very porous, and had twice as much surface binding capacity for avidin as the sputtered electrode. The results were rationalized by the assumption of different packing density of avidin inner layer on the two surfaces, and consequently different electron transfer distance between the electrode and Ru-bipy on the IgG outer layer. A linear relationship between electrochemical current and IgG concentration was obtained in the range of 40-4000 nmol L −1 on the nanoparticle-based electrode. The approach can be employed in the electrochemical detection of immunoassays using non-enzymatic redox labels.

show abstract

“…The EC transduction of the hybridization event relies typically on external electrochemically-active indicators, which comprise DNA intercalators (metal complexes, 24,25 anthracycline antibiotics, 26 and bisbenzimide dyes 27,28 ), enzymes, 29,30 and metal nanoparticles. [31][32][33] Despite the extensive research efforts in EC hybridization transduction, just a few groups, 28,29,34 including our group, have devoted to PCR product detection. Moreover, to the best of our knowledge, the integration of microchip-based PCR with EC detection functionality has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Microfabricated PCR-electrochemical device for simultaneous DNA amplification and detection

2003

View full text Add to dashboard Cite

Microfabricated silicon/glass-based devices with functionalities of simultaneous polymerase chain reaction (PCR) target amplification and sequence-specific electrochemical (EC) detection have been successfully developed. The microchip-based device has a reaction chamber (volume of 8 µl) formed in a silicon substrate sealed by bonding to a glass substrate. Electrode materials such as gold and indium tin oxide (ITO) were patterned on the glass substrate and served as EC detection platforms where DNA probes were immobilized. Platinum temperature sensors and heaters were patterned on top of the silicon substrate for real-time, precise and rapid thermal cycling of the reaction chamber as well as for efficient target amplification by PCR. DNA analyses in the integrated PCR-EC microchip start with the asymmetric PCR amplification to produce single-stranded target amplicons, followed by immediate sequence-specific recognition of the PCR product as they hybridize to the probe-modified electrode. Two electrochemistry-based detection techniques including metal complex intercalators and nanogold particles are employed in the microdevice to achieve a sensitive detection of target DNA analytes. With the integrated PCR-EC microdevice, the detection of trace amounts of target DNA (as few as several hundred copies) is demonstrated. The ability to perform DNA amplification and EC sequence-specific product detection simultaneously in a single reaction chamber is a great leap towards the realization of a truly portable and integrated DNA analysis system.

show abstract

Gold Nanoparticle-Based Quantitative Electrochemical Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband Electrodes

Cited by 320 publications

References 45 publications

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Enhanced electrochemical activity of redox-labels in multi-layered protein films on indium tin oxide nanoparticle-based electrode

Microfabricated PCR-electrochemical device for simultaneous DNA amplification and detection

Contact Info

Product

Resources

About