Gold nanoparticle-based probes for the colorimetric detection of Mycobacterium avium subspecies paratuberculosis DNA

Ganareal, Thenor Aristotile Charles S.; Balbin, Michelle M.; Monserate, Juvy J.; Salazar, Joel R.; Mingala, Claro N.

doi:10.1016/j.bbrc.2018.01.033

Cited by 16 publications

(15 citation statements)

References 25 publications

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“…The minimum value that could be detected by their method was 103 ng ml À1 . 17 This study is similar to our study in terms of the type of the aggregation inducer (MgCl 2 ) and the identication of non-amplied DNA, but the probe used was 22 nucleotides in length and unlike ours, the probe had no spacer. For this reason, the minimum diagnostic value of their method was 103 ng ml À1 , but this value in our study was 50 ng ml À1 .…”

Section: Spectral Analysissupporting

confidence: 77%

“…11,12 Recently, researchers have shown great interest in using gold nanoparticles to identify pathogens. [13][14][15][16][17][18][19][20] This method of detection is based on the property of surface plasmon resonance (SPR) of gold nanoparticles. The change in the dispersion state of the nanoparticles results in a color change, which results in detection.…”

Section: Introductionmentioning

confidence: 99%

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Design of a gold nanoprobe for the detection ofPseudomonas aeruginosaelastase gene (lasB)

2020

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Section: Spectral Analysissupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Design of a gold nanoprobe for the detection ofPseudomonas aeruginosaelastase gene (lasB)

2020

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“…This DNA-based AuNPs method is completely opposite compared with the method previously developed and optimized by Ganareal et al [8] as they utilized modified AuNPs thru bio functionalization process. In the case of positive samples for AHPND, coloration stays pink red upon the addition of salt denoting AuNPs-probe stability due to its hybridization with complementary DNA.…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticle-based detection of pirAvp toxin gene causing acute hepatopancreatic necrosis disease (AHPND)

et al. 2020

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Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio species is one of the most common bacterial diseases carrying the plasmid encoding pirA vp and pirB vp toxin genes that cause lethargic and anorexic effect on infected shrimps due to induced necrosis in the hepatopancreas. This study demonstrates the use of gold nanoparticles (AuNPs) coupled with specific thiol probe for the detection of pirA vp toxin gene causing AHPND. Detection of pirA vp toxin gene using thiol probe was validated using colorimetric assay. The validation results showed that the developed Deoxyribonucleic acid (DNA)-based AuNPs detection was specific and highly sensitive to detect the pirA vp toxin gene at 20 fg/ul of shrimp genomic DNA. This nanotech-based polymerase chain reaction (PCR) amplification-free protocol can serve as a new means of diagnostic strategy for AHPND detection in aquaculture industry. Furthermore, the method provides accurate and timely detection on field setting which can help to reduce mass mortality and drastic economic losses on aquaculture.

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“…Nanotechnology is an emerging science and is the study of matter with one or more dimensions in between 1 to 100 nm. The combination of nanoscience and biotechnology has created a new growing field of research in the form of nanobiotechnology with massive opportunities [5] to further improve healthcare, medical treatments, therapeutics and biomedical [7] uses such as radiotherapy enhancers [8][9][10], drug and gene delivery vehicles, and highly specific and sensitive diagnostic assays [11,12]. Among all noble metals, gold (Au) and silver (Ag) are the most extensively studied due to the well-established synthesis routes, their relatively higher content in the earth's crust and better safety profile.…”

Section: Introductionmentioning

confidence: 99%

“…Gold nanoparticles (AuNPs), commonly known as colloidal gold or gold colloids, are the most stable metal nanoparticle. AuNPs present distinctive characteristics like size-related optical, electronic and magnetic properties, individual particle behavior and specially, compatibility with biomolecules [10,11]. These characteristics of AuNPs attracted researchers from the field of human and animal medicine to apply these properties in a point-of-care or field diagnosis of various infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

Application of Noble Metals in the Advances in Animal Disease Diagnostics

Tubalinal¹,

Lucero²,

Mangahas³

et al. 2022

Updates on Veterinary Anatomy and Physiology

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The advent of molecular biology and biotechnology has given ease and comfort for the screening and detection of different animal diseases caused by bacterial, viral, and fungal pathogens. Furthermore, detection of antibiotics and its residues has advanced in recent years. However, most of the process of animal disease diagnostics is still confined in the laboratory. The next step to conduct surveillance and prevent the spread of animal infectious diseases is to detect these diseases in the field. Through the discovery and continuous development in the field of nanobiotechnology, it was found that incorporation of noble metal nanoparticles to biotechnology tools such as the loop-mediated isothermal amplification (LAMP), lateral flow assays (LFAs) and dipsticks provided a promising start to conduct point-of-care diagnostics. Moreover, the modification and application of nanoparticle noble metals has increased the stability, effectiveness, sensitivity and overall efficacy of these diagnostic tools. Thus, recent advances in disease diagnostics used these noble metals such as gold, silver and platinum.

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Gold nanoparticle-based probes for the colorimetric detection of Mycobacterium avium subspecies paratuberculosis DNA

Cited by 16 publications

References 25 publications

Design of a gold nanoprobe for the detection ofPseudomonas aeruginosaelastase gene (lasB)

Design of a gold nanoprobe for the detection ofPseudomonas aeruginosaelastase gene (lasB)

Gold nanoparticle-based detection of pirAvp toxin gene causing acute hepatopancreatic necrosis disease (AHPND)

Application of Noble Metals in the Advances in Animal Disease Diagnostics

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