Gold-nanoparticle based electrochemical DNA sensor for the detection of fish pathogen Aphanomyces invadans

G, Kuan; Liew, Pei Sheng; Rijiravanich, Patsamon; Krishnaveni, M.; Ravichandran, Manickam; Yin, Lee Su; Lertanantawong, Benchaporn; Surareungchai, Werasak

doi:10.1016/j.talanta.2013.09.016

Cited by 39 publications

(18 citation statements)

References 20 publications

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“…Table 3 is a summary of various biosensors for detecting fish pathogens. They include quartz crystal microbalance (QCM), the microcantilever sensor, amperometric sensor, potentiometric sensor, and surface plasmon resonance (SPR) biosensor, and lateral flow tests, targeting either viral RNA [92][93][94] or the bacteria cells [95][96][97][98][99]. Biosensors are typically designed to detect known bacteria or virus, but less so for identification of unknown.…”

Section: Biosensorsmentioning

confidence: 99%

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sutarlie

Loh

2020

Research

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In aquaculture industry, fish, shellfish, and aquatic plants are cultivated in fresh, salt, or brackish waters. The increasing demand of aquatic products has stimulated the rapid growth of aquaculture industries. How to effectively monitor and control water quality is one of the key concerns for aquaculture industry to ensure high productivity and high quality. There are four major categories of water quality concerns that affect aquaculture cultivations, namely, (1) physical parameters, e.g., pH, temperature, dissolved oxygen, and salinity, (2) organic contaminants, (3) biochemical hazards, e.g., cyanotoxins, and (4) biological contaminants, i.e., pathogens. While the physical parameters are affected by climate changes, the latter three are considered as environmental factors. In this review, we provide a comprehensive summary of sensors, biosensors, and analytical technologies available for monitoring aquaculture water quality. They include low-cost commercial sensors and sensor network setups for physical parameters. They also include chromatography, mass spectrometry, biochemistry, and molecular methods (e.g., immunoassays and polymerase chain reaction assays), culture-based method, and biophysical technologies (e.g., biosensors and nanosensors) for environmental contamination factors. According to the different levels of sophistication of various analytical techniques and the information they can provide (either fine fingerprint, highly accurate quantification, semiquantification, qualitative detection, or fast screening), we will comment on how they may be used as complementary tools, as well as their potential and gaps toward current demand of real-time, online, and/or onsite detection.

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Section: Biosensorsmentioning

confidence: 99%

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sutarlie

Loh

2020

Research

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“…A burgundy red colloidal solution was formed after approximately 30 min of reaction, indicating the formation of AuNPs. The resulting gold colloid solution was then left to cool to room temperature before it was centrifuged for 20 min at 4 • C with a centrifugal force (RCF) of 11,930× g. Poly(styrene-co-acrylic acid) (PSA), i.e., the latex microspheres, on the other hand, was synthesized as described by Kuan et al [11] with slight modifications. Firstly, 190 g of Milli-Q water was purged with N 2 gas in a three-necked flask submerged in a water bath for 1 h under continuous stirring at 350 rpm.…”

Section: Preparation Of Gold-latex Particlesmentioning

confidence: 99%

Sandwich-Type DNA Micro-Optode Based on Gold–Latex Spheres Label for Reflectance Dengue Virus Detection

Jeningsih

Tan

Ulianas

et al. 2020

Sensors

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A DNA micro-optode for dengue virus detection was developed based on the sandwich hybridization strategy of DNAs on succinimide-functionalized poly(n-butyl acrylate) (poly(nBA-NAS)) microspheres. Gold nanoparticles (AuNPs) with an average diameter of ~20 nm were synthesized using a centrifugation-based method and adsorbed on the submicrometer-sized polyelectrolyte-coated poly(styrene-co-acrylic acid) (PSA) latex particles via an electrostatic method. The AuNP–latex spheres were attached to the thiolated reporter probe (rDNA) by Au–thiol binding to functionalize as an optical gold–latex–rDNA label. The one-step sandwich hybridization recognition involved a pair of a DNA probe, i.e., capture probe (pDNA), and AuNP–PSA reporter label that flanked the target DNA (complementary DNA (cDNA)). The concentration of dengue virus cDNA was optically transduced by immobilized AuNP–PSA–rDNA conjugates as the DNA micro-optode exhibited a violet hue upon the DNA sandwich hybridization reaction, which could be monitored by a fiber-optic reflectance spectrophotometer at 637 nm. The optical genosensor showed a linear reflectance response over a wide cDNA concentration range from 1.0 × 10−21 M to 1.0 × 10−12 M cDNA (R2 = 0.9807) with a limit of detection (LOD) of 1 × 10−29 M. The DNA biosensor was reusable for three consecutive applications after regeneration with mild sodium hydroxide. The sandwich-type optical biosensor was well validated with a molecular reverse transcription polymerase chain reaction (RT-PCR) technique for screening of dengue virus in clinical samples, e.g., serum, urine, and saliva from dengue virus-infected patients under informed consent.

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“…A saturated calomel electrode was used as the reference electrode, a platinum wire was used as the counter electrode and DNA modified gold electrode was applied as the working electrode. square wave voltammetry triplex forming oligonucleotides as the recognizing probes 3 × 10 À 10 to 3 × 10 À 6 3 × 10 À 9 [15] differential pulse voltammetry graphene quantum dots modified glassy carbon electrode 10 À 8 to 5 × 10 À 7 10 À 9 [16] electrochemiluminescence stem-loop structured capture DNA probe 10 À 12 to 10 À 7 5 × 10 À 13 [17] amperometric current target-induced structural switching and enzymatic polymerization 10 À 13 to 10 À 7 10 À 13 [18] differential pulse voltammetry target-triggered hemin/G-quadruplex 10 À 13 to 10 À 9 5.4 × 10 À 14 [19] square wave voltammetry toehold-triggered structure switchable signaling 10 À 13 to 10 À 6 4.2 × 10 À 14 [20] electrochemical impedance spectroscopy self-healing 3D nanohybrid of AuNPs and reduced graphene oxide 10 À 13 to 10 À 7 3.64 × 10 À 14 [21] differential pulse voltammetry screen-printed graphite surface with embedded bismuth precursor 10 À 13 to 10 À 8 3 × 10 À 14 [22] chronocoulometry in situ grown DNA nanotail 10 À 13 to 10 À 11 2 × 10 À 14 [23] square wave voltammetry star trigon structure-aided DNA walker 10 À 15 to 10 À 10 10 À 15 [24] amperometry double tetrahedral DNA 10 À 15 to 10 À 8 10 À 15 [25] differential pulse anodic stripping voltammetry DNA modified gold-latex spheres 5 × 10 À 15 to 5 × 10 À 12 5 × 10 À 16 [26] square wave voltammetry DNA three-way junction for target recycling 10 À 15 to 10 À 11 4.1 × 10 À 16 this work Table 2. DNA sequences used in this study.…”

Section: Electrochemical Measurementsmentioning

confidence: 99%

Ultrasensitive Detection of ctDNA by Target‐Mediated In Situ Growth of DNA Three‐Way Junction on the Electrode

2019

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Circulation tumor DNA (ctDNA) is an emerging biomarker for diagnosis and therapy of cancers. Herein, an ultrasensitive electrochemical method for ctDNA quantification is developed by in situ growth of DNA three‐way junction on the electrode. Three hairpin‐structured DNA probes are used as fuel strands and strand‐displacement polymerization reactions are initiated by target ctDNA for the in situ growth of the DNA junction structure, which changes the electron transfer pathways of electrochemical species. By further employing a reductant‐mediated amplification, the method shows sub‐femtomolar sensitivity. Meanwhile, specific analysis of ctDNA is achieved with negligible interference by DNAs with one or more mismatched sites. Therefore, the proposed method may have great analytical and clinical applications.

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Gold-nanoparticle based electrochemical DNA sensor for the detection of fish pathogen Aphanomyces invadans

Cited by 39 publications

References 20 publications

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality

Sandwich-Type DNA Micro-Optode Based on Gold–Latex Spheres Label for Reflectance Dengue Virus Detection

Ultrasensitive Detection of ctDNA by Target‐Mediated In Situ Growth of DNA Three‐Way Junction on the Electrode

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