Gold nanoparticle‐based brachytherapy enhancement in choroidal melanoma using a full Monte Carlo model of the human eye

Asadi, Somayeh; Vaezzadeh, Majid; Masoudi, S. Farhad; Rahmani, Faezeh; Knaup, Courtney; Meigooni, Ali S.

doi:10.1120/jacmp.v16i5.5568

Cited by 37 publications

(43 citation statements)

References 60 publications

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“…However, the results are still controversial regarding the involved and prominent interaction processes of ionizing radiation with the GNPs, which have effects on dose enhancement factor (DEF) in tumor. Until now, the most effective parameters have been investigated such as the dimension of nanoparticles, high molar concentrations, and the lower energies of the photons or gamma rays to obtain the higher doses by experimental dosimetry and MC methods [3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19]. …”

Section: Purposementioning

confidence: 99%

Investigation of the gold nanoparticles effects on the prostate dose distribution in brachytherapy: gel dosimetry and Monte Carlo method

Khosravi¹,

Hashemi²,

Rahmani³

et al. 2016

jcb

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PurposeIn this work, gold nanoparticles (GNPs) were embedded in the MAGIC-f polymer gel irradiated with the 192Ir brachytherapy sources.Material and methodsAt the first plexiglas phantom was made as the human pelvis. The GNPs were synthesized with 15 nm in diameter and 0.1 mM (0.0197 mg/ml) in concentration by using a chemical reduction method. Then, the MAGIC-f gel was synthesized. The fabricated gel was poured into the tubes located at the prostate (with and without the GNPs) locations of the phantom. The phantom was irradiated with 192Ir brachytherapy sources for prostate cancer. After 24 hours, the irradiated gels was read by using Siemens 1.5 Tesla MRI scanner. Following the brachytherapy practices, the absolute doses at the reference points and isodose curves were extracted and compared by experimental measurements and Monte Carlo (MC) simulations.ResultsThe mean absorbed doses in the presence of the GNPs in prostate were 14% higher than the corresponding values without the GNPs in the brachytherapy. The gamma index analysis (between gel and MC) using 7%/7 mm was also applied to the data and a high pass rate achieved (91.7% and 86.4% for analysis with/without GNPs, respectively).ConclusionsThe real three-dimensional analysis shows the comparison of the dose-volume histograms measured for planning volumes and the expected one from the MC calculation. The results indicate that the polymer gel dosimetry method, which developed and used in this study, could be recommended as a reliable method for investigating the dose enhancement factor of GNPs in brachytherapy.

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Section: Purposementioning

confidence: 99%

Investigation of the gold nanoparticles effects on the prostate dose distribution in brachytherapy: gel dosimetry and Monte Carlo method

Khosravi¹,

Hashemi²,

Rahmani³

et al. 2016

jcb

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“…OCM-1 cells (1x10 5 ) were seeded in six-well plates and treated with different concentrations of GNA (0, 0.75, 1.5, and 3 µM). Briefly, cells were harvested at 24 and 48 h post-treatment with GNA.…”

Section: Methodsmentioning

confidence: 99%

“…Cell cycle distribution was analyzed using the FACSCalibur using Cell FIT software (Easy curve Fit 2.0) (both from BD Biosciences, San Jose, CA, USA). 5 ) were grown in six-well plates, which were incubated with GNA (0, 0.75, 1.5, 3 µM) with or without LY294002 (15 mΜ; Sigma-Aldrich; Merck Millipore) for 24 h. LY294002, a specific inhibitor of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway, is able to significantly repress Akt phosphorylation (15). Total RNA was isolated from cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treated with DNase I. Purified RNA at density of 0.5 µg/µl with nuclease-free water was used for cDNA synthesis with the PrimeScript First Strand cDNA Synthesis kit (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturers protocol.…”

Section: Methodsmentioning

confidence: 99%

Gambogenic acid induces cell growth inhibition, cell cycle arrest and metastasis inhibition in choroidal melanoma in a dose-dependent manner

Wang

Yan

2017

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to explore the effects of gambogenic acid (GNA) on the malignant behaviors of choroidal melanoma cells, including cell viability, cell cycle, migration and invasion, and to elucidate the underlying regulatory mechanism. The human choroidal melanoma cell line OCM-1 was treated with different concentrations of GNA and cell viability, colony formation ability, cell cycle, migration and invasion were analyzed. Additionally, cells were incubated with or without LY294002, a specific inhibitor of the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway, for 24 h. Levels of cell cycle-associated proteins (cyclin D1, cyclin E, cyclin-dependent kinase 2 and P21), epithelial-mesenchymal transition (EMT)-associated molecules (epithelial-cadherin, α-smooth muscle actin and vimentin) and phosphorylated (p)-AKT/AKT were determined using reverse transcription-quantitative polymerase chain reaction and western blot analysis. The results demonstrated that GNA significantly inhibited cell viability and induced cell cycle arrest at the G0/G1 phase in a dose-dependent manner (P<0.01). Furthermore, GNA administration significantly suppressed cell migration and invasion in a dose-dependent manner (P<0.01). Treatment with GNA or LY294002 induced a marked decrease in the expression of p-AKT/AKT, a significant downregulation in cell cycle-associated molecules (P<0.01), and a significant decrease in cell viability (P<0.01). Co-treatment with LY294002 and GNA had an additive effect on the growth of OCM-1 cells. In conclusion, the results of the present study suggest that treatment with GNA may inhibit cell viability and induce G0/G1 arrest. Furthermore, GNA may also inhibit cell metastasis via regulating EMT-associated molecules. The PI3K/Akt signaling pathway may be a key mechanism involved in the progression of choroidal melanoma, and GNA may serve as a potential therapeutic reagent for the treatment of this disease.

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“…In recent MC studies,19, 20‎ ‎the effects of GNPs on the brachytherapy for choroidal melanoma, have been investigated using iodine ( 125 I) and palladium ( 103 Pd), the most common irradiation sources for this type of intraocular tumor. These simulations were performed with the eye model phantom and the water phantom to determine the dose enhancement factor (DEF) in the presence and in the absence of GNPs using the mentioned brachytherapy sources.…”

Section: Introductionmentioning

confidence: 99%

Gamma irradiation of ocular melanoma and lymphoma cells in the presence of gold nanoparticles: in vitro study

Kanavi

Asadi

Balagholi

et al. 2018

J Applied Clin Med Phys

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The aim of this work was to determine whether conjugation of cultivated choroidal melanoma and Burkitt's lymphoma cells with gold nanoparticles (GNPs) is beneficial for these series of ocular cancer patients. GNPs are radiosensitizers and can sensitize tumors to radiotherapy.This application has been examined in several tumor types, but not in choroidal melanoma. This study shows the results of in vitro study on the choroidal melanoma and also Burkitt's lymphoma cells in the presence of GNPs during continuous gamma irradiation. Cytotoxicity of GNPs were assessed for five different concentrations then cultured melanoma and Burkitt's lymphoma cells were irradiated with a Gamma source in the presence and absence of NPs. Incubation of melanoma cells with GNP concentrations below 100 μg/ml, accompanied by gamma irradiation, increased cell death (P value = 0.016) . In the absence of irradiation, GNPs at these concentrations did not affect cultured melanoma cell metabolism. Reduced cell viability resulted from a significant increase in absorbed energy by the tumor. Moreover, GNP concentrations higher than 200 μg/ml induced cytotoxicity in melanoma cells. Cytotoxicity assay in GNPs‐loaded Burkitt's lymphoma cells showed a slight decrease in cell viability at 50 μg/ml and clear cytotoxicity at concentrations higher than 100 μg/ml (P value = 0.035). Concentration and proper injection doses of GNPs in sensitive tissues such as the human eye are important variables yet to be determined.This is the first report of choroidal melanoma dosimetry performed in the presence of GNPs and provides valuable insights into future therapeutic approaches. Further in vitro study with more different sizes and concentrations is needed to determine the optimum size and concentration before any clinical research in this regard.

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Gold nanoparticle‐based brachytherapy enhancement in choroidal melanoma using a full Monte Carlo model of the human eye

Cited by 37 publications

References 60 publications

Investigation of the gold nanoparticles effects on the prostate dose distribution in brachytherapy: gel dosimetry and Monte Carlo method

Investigation of the gold nanoparticles effects on the prostate dose distribution in brachytherapy: gel dosimetry and Monte Carlo method

Gambogenic acid induces cell growth inhibition, cell cycle arrest and metastasis inhibition in choroidal melanoma in a dose-dependent manner

Gamma irradiation of ocular melanoma and lymphoma cells in the presence of gold nanoparticles: in vitro study

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