Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

Shiota, Megumi; Naya, Masayuki; Yamamoto, Takehiro; Hishiki, Takako; Tani, Tohru; Takahashi, Hiroyuki; Kubo, Akiko; Koike, Daisuke; Itoh, Mai; Ohmura, Mitsuyo; Kabe, Yoshio; Sugiura, Yuki; Hiraoka, Nobuyoshi; Morikawa, Takayuki; Takubo, Keiyo; Suina, Kentaro; Nagashima, Hideaki; Sampetrean, Oltea; Nagano, Osamu; Saya, Hideyuki; Yamazoe, Shogo; Watanabe, Hiroyuki; Suematsu, Makoto

doi:10.1038/s41467-018-03899-1

Cited by 78 publications

(63 citation statements)

References 68 publications

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“…Wrobel et al (2014) reported that the levels of sulfane sulfur (polysulfides) in gliomas with the highest grades of malignancy are greater than those of glioma-free brain regions. A similar result was also reported in glioblastoma-bearing ipsilateral hemispheres, which contain greater amounts of H 2 S n than the glioblastoma-free control hemispheres (Shiota et al, 2018). These observations suggest that sulfane sulfur accumulation may play an important role in glioma cell proliferation and that its dependence on sulfane sulfur is greater in higher grades of malignancy.…”

Section: Production Of Cysssh and Gsshsupporting

confidence: 83%

Signalling by hydrogen sulfide and polysulfides via protein S‐sulfuration

Kimura

2019

British J Pharmacology

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Hydrogen sulfide (H2S) is a signalling molecule that regulates neuronal transmission, vascular tone, cytoprotection, inflammatory responses, angiogenesis, and oxygen sensing. Some of these functions have recently been ascribed to its oxidized form polysulfides (H2Sn), which can be produced by 3‐mercaptopyruvate sulfurtransferase (MPST), also known as a H2S‐producing enzyme. H2Sn activate ion channels, tumour suppressors, transcription factors, and protein kinases. H2Sn S‐sulfurate (S‐sulfhydrate) cysteine residues of these target proteins to modify their activity by inducing conformational changes through the formation of a disulfide bridge between the two cysteine residues involved. The chemical interaction between H2S and NO also generates H2Sn, which may be a chemical entity that exerts the synergistic effect of H2S and NO. MPST also produces redox regulators cysteine persulfide (CysSSH), GSH persulfide (GSSH), and persulfurated proteins. In addition to MPST, haemoproteins such as haemoglobin, myoglobin, neuroglobin, and catalase as well as SOD can produce H2Sn, and sulfide quinone oxidoreductase and cysteinyl tRNA synthetase can make GSSH and CysSSH. This review focuses on the recent progress in the study of the production and physiological roles of these persulfurated and polysulfurated molecules. Linked Articles This article is part of a themed section on Hydrogen Sulfide in Biology & Medicine. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v177.4/issuetoc

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Section: Production Of Cysssh and Gsshsupporting

confidence: 83%

Signalling by hydrogen sulfide and polysulfides via protein S‐sulfuration

Kimura

2019

British J Pharmacology

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“…3-MST expression was also clearly demonstrated in many different primary tumor tissues, including human brain gliomas [33], human melanoma specimens [34], human colon cancer…”

Section: Discussionmentioning

confidence: 92%

Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics in Murine Colon Cancer Cells

et al. 2020

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3-mercaptopyruvate sulfurtransferase (3-MST) has emerged as one of the significant sources of biologically active sulfur species in various mammalian cells. The current study was designed to investigate the functional role of 3-MST's catalytic activity in the murine colon cancer cell line CT26. The novel pharmacological 3-MST inhibitor HMPSNE was used to assess cancer cell proliferation, migration and bioenergetics in vitro. Methods included measurements of cell viability (MTT and LDH assays), cell proliferation and in vitro wound healing (IncuCyte) and cellular bioenergetics (Seahorse extracellular flux analysis). 3-MST expression was detected by Western blotting; H 2 S production was measured by the fluorescent dye AzMC. The results show that CT26 cells express 3-MST protein and mRNA, as well as several enzymes involved in H 2 S degradation (TST, ETHE1). Pharmacological inhibition of 3-MST concentration-dependently suppressed H 2 S production and, at 100 and 300 µM, attenuated CT26 proliferation and migration. HMPSNE exerted a bell-shaped effect on several cellular bioenergetic parameters related to oxidative phosphorylation, while other bioenergetic parameters were either unaffected or inhibited at the highest concentration of the inhibitor tested (300 µM). In contrast to 3-MST, the expression of CBS (another H 2 S producing enzyme which has been previously implicated in the regulation of various biological parameters in other tumor cells) was not detectable in CT26 cells and pharmacological inhibition of CBS exerted no significant effects on CT26 proliferation or bioenergetics. In summary, 3-MST catalytic activity significantly contributes to the regulation of cellular proliferation, migration and bioenergetics in CT26 murine colon cancer cells. The current studies identify 3-MST as the principal source of biologically active H 2 S in this cell line.

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“…[64c] A similar nanostructure type has also been synthesized by gold nanoparticles sliding on nanohoodoos and forming clusters via deposition and evaporation of deionized water (Figure 7c-e). [58] Copyright 2018, Nature Publishing Group. In addition, the vertical spreading of solution droplets often results in random adsorption of molecules across the entire nanostructure surface which has varying levels of SERS activities, thus eventually leading to poor experimental reproducibility and detection sensitivity.…”

Section: Ordered Nanostructuresmentioning

confidence: 99%

“…Nanostructures-on-f ilm Gold-nanofève substrates [58] Deposition EF ≈ 10 7 SR ≈ 11.0% • Ultrasensitive, quantitative analysis of biomolecules via direct SERS measurements 3D cross-point plasmonic nanoarchitectures [66] Nanotransfer printing…”

Section: -Mpy • Sensor Platform For Nanosized Biomolecules • Sers Namentioning

confidence: 99%

Engineering State‐of‐the‐Art Plasmonic Nanomaterials for SERS‐Based Clinical Liquid Biopsy Applications

et al. 2019

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Precision oncology, defined as the use of the molecular understanding of cancer to implement personalized patient treatment, is currently at the heart of revolutionizing oncology practice. Due to the need for repeated molecular tumor analyses in facilitating precision oncology, liquid biopsies, which involve the detection of noninvasive cancer biomarkers in circulation, may be a critical key. Yet, existing liquid biopsy analysis technologies are still undergoing an evolution to address the challenges of analyzing trace quantities of circulating tumor biomarkers reliably and cost effectively. Consequently, the recent emergence of cutting-edge plasmonic nanomaterials represents a paradigm shift in harnessing the unique merits of surface-enhanced Raman scattering (SERS) biosensing platforms for clinical liquid biopsy applications. Herein, an expansive review on the design/synthesis of a new generation of diverse plasmonic nanomaterials, and an updated evaluation of their demonstrated SERS-based uses in liquid biopsies, such as circulating tumor cells, tumor-derived extracellular vesicles, as well as circulating cancer proteins, and tumor nucleic acids is presented. Existing challenges impeding the clinical translation of plasmonic nanomaterials for SERS-based liquid biopsy applications are also identified, and outlooks and insights into advancing this rapidly growing field for practical patient use are provided.

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Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

Cited by 78 publications

References 68 publications

Signalling by hydrogen sulfide and polysulfides via protein S‐sulfuration

Signalling by hydrogen sulfide and polysulfides via protein S‐sulfuration

Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics in Murine Colon Cancer Cells

Engineering State‐of‐the‐Art Plasmonic Nanomaterials for SERS‐Based Clinical Liquid Biopsy Applications

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