Gold nanoclusters for Parkinson's disease treatment

Gao, Guanbin; Chen, Rui; He, Meng Xiao; Li, Jing; Wang, Liyun; Sun, Taolei

doi:10.1016/j.biomaterials.2018.12.013

Cited by 108 publications

(76 citation statements)

References 56 publications

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“…[27][28][29] Besides, the outstanding photo-stability, long carrier lifetime and Stokes shi, and efficient renal clearance properties make AuNCs ideal choices for analysis and bio-imaging. 30,31 On the other hand, the relatively weak luminescence and hardly adjustable emission have been a non-negligible hindrance to practical applications. 32 To date, the specic luminescence mechanism of AuNCs has remained controversial.…”

Section: Introductionmentioning

confidence: 99%

Cucurbiturils brighten Au nanoclusters in water

Jiang

Wang

et al. 2020

Chem. Sci.

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Section: Introductionmentioning

confidence: 99%

Cucurbiturils brighten Au nanoclusters in water

Jiang

Wang

et al. 2020

Chem. Sci.

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“…25 Compared to CuNCs, AuNCs are much more stable and little ions can be released in bioculture. 26 The AuNCs have shown higher safety and efficiency for therapy of in vivo diseases, such as the cancers, 27 bacterial infections 28 and Parkinsons, 29 compared to other nanomedicines including CuNCs and larger AuNPs. They can be cleaned from the body efficiently because of their ultra-small sizes.…”

Section: Introductionmentioning

confidence: 99%

<p>Ultra-Small Lysozyme-Protected Gold Nanoclusters as Nanomedicines Inducing Osteogenic Differentiation</p>

Li¹,

Zhuang²,

Tao³

et al. 2020

IJN

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Ultra-small gold nanoclusters (AuNCs), as emerging fluorescent nanomaterials with excellent biocompatibility, have been widely investigated for in vivo biomedical applications. However, their effects in guiding osteogenic differentiation have not been investigated, which are important for osteoporosis therapy and bone regeneration. Herein, for the first time, lysozymeprotected AuNCs (Lys-AuNCs) are used to stimulate osteogenic differentiation, which have the potential for the treatment of bone disease. Methods: Proliferation of MC3T3E-1 is important for osteogenic differentiation. First, the proliferation rate of MC3T3E-1 was studied by Cell Counting Kit-8 (CCK8) assays. Signaling pathways of PI3K/Akt play central roles in controlling proliferation throughout the body. The expression of PI3K/Akt was investigated in the presence of lysozyme, and lysozyme-protected AuNCs (Lys-AuNCs) by Western blot (WB) and intracellular cell imaging to evacuate the osteogenic differentiation mechanisms. Moreover, the formation of osteoclasts (OC) plays a negative role in the differentiation of osteoblasts. Nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) signaling pathways are used to understand the negative influence of the osteogenic differentiation by the investigation of Raw 264.7 cell line. Raw 264.7 (murine macrophage-like) cells and NIH/3T3 (mouse fibroblast) cells were treated with tyloxapol, and the cell viability was assessed. Raw 264.7 cells have also been used for in vitro studies, on understanding the osteoclast formation and function. The induced osteoclasts were identified by TRAP confocal fluorescence imaging. These key factors in osteoclast formation, such as (NFATc-1, c-Fos, V-ATPase-2 and CTSK), were investigated by Western blot. Results: Based on the above investigation, Lys-AuNCs were found to promote osteogenic differentiation and decrease osteoclast activity. It is noteworthy that the lysozyme (protected template), AuNPs, or the mixture of Lysozyme and AuNPs have negligible effects on osteoblastic differentiation compared to Lys-AuNCs. Conclusion: This study opens up a novel avenue to develop a new gold nanomaterial for promoting osteogenic differentiation. The possibility of using AuNCs as nanomedicines for the treatment of osteoporosis can be expected.

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“…An enhanced absorption at 275 nm occurs because of the ligand‐to‐metal charge transfer (LMCT: S→Au) mixed with ligand‐to‐metal‐metal charge transfer (LMMCT: S→Au···Au) absorption (Figure S1c, Supporting Information). The formation of supramolecules leads to a smaller Au(I) – S – Au(I) angle then a stronger aurophilic Au(I)···Au(I) interactions are conducted, enhancing LMMCT absorption . In addition, energy‐dispersive X‐ray spectroscopy (EDS) was carried out to identify the component of ‐[BSA‐Au(I)] n ‐supramolecules.…”

Section: Resultsmentioning

confidence: 99%

“…The formation of supramolecules leads to a smaller Au(I) -S -Au(I) angle then a stronger aurophilic Au(I)···Au(I) interactions are conducted, enhancing LMMCT absorption. [43][44][45][46] In addition, energy-dispersive X-ray spectroscopy (EDS) was carried out to identify the component of -[BSA-Au(I)] n -supramolecules. As shown in Figure S2c, Supporting Information, the weight ratio of Au: S of the supramolecule is 85.2%: 14.8%.…”

Section: Evaluation Of High Luminescence Bsa-auncsmentioning

confidence: 99%

Supramolecules‐Guided Synthesis of Brightly Near‐Infrared Au₂₂ Nanoclusters with Aggregation‐Induced Emission for Bioimaging

Wei

Luan

Gao

et al. 2019

Part & Part Syst Charact

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Protein‐protected gold nanoclusters with emission in the near‐infrared wavelength range have been widely considered for applications in biomedical fields. However, their quantum yield (QY) remains low, thus limiting their practical applications. Herein, a novel strategy to synthesize bovine serum albumin–encapsulated gold nanoclusters (BSA‐AuNCs) with QY of 23% is developed. Assembled coordination polymers (supramolecules) of Au(I)‐BSA complexes are initially formed because of the intermolecular forces between BSA ligands. The forces are easily controlled by pH level during the reaction, leading to significant change in the photoluminescence of BSA‐AuNCs. By regulating the pH and reaction temperature, Au(0)@Au(I) core‐shell structured BSA‐AuNCs are fabricated in 2 h. Importantly, such AuNCs are in a rigidified state with high Au(I) content in the shell, offering an explanation for their high luminescence character. Further increasing QY to 29% is achieved by confining BSA‐AuNCs into a cationic polymer, poly(allylamine) hydrochloride (AuNCs@PAH). Enhanced cellular uptake and improved sensitivity of AuNCs@PAH to glutathione compared to BSA‐AuNCs is demonstrated. These findings may give insights into the synergistic effect of pH level and reaction temperature on the properties of protein‐encapsulated AuNCs and provide a possible way for up‐scaled fabrication of brighter AuNCs protected by other protein templates.

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Gold nanoclusters for Parkinson's disease treatment

Cited by 108 publications

References 56 publications

Cucurbiturils brighten Au nanoclusters in water

Cucurbiturils brighten Au nanoclusters in water

<p>Ultra-Small Lysozyme-Protected Gold Nanoclusters as Nanomedicines Inducing Osteogenic Differentiation</p>

Supramolecules‐Guided Synthesis of Brightly Near‐Infrared Au₂₂ Nanoclusters with Aggregation‐Induced Emission for Bioimaging

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Gold nanoclusters for Parkinson's disease treatment

Cited by 108 publications

References 56 publications

Cucurbiturils brighten Au nanoclusters in water

Cucurbiturils brighten Au nanoclusters in water

<p>Ultra-Small Lysozyme-Protected Gold Nanoclusters as Nanomedicines Inducing Osteogenic Differentiation</p>

Supramolecules‐Guided Synthesis of Brightly Near‐Infrared Au22 Nanoclusters with Aggregation‐Induced Emission for Bioimaging

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Supramolecules‐Guided Synthesis of Brightly Near‐Infrared Au₂₂ Nanoclusters with Aggregation‐Induced Emission for Bioimaging