Gold coated ferric oxide nanoparticles based disposable magnetic genosensors for the detection of DNA hybridization processes

Loaiza, Óscar A.; Jubete, Elena; Ochoteco, Estíbalitz; Cabañero, Germán; Grande, Hans J.; Rodríguez, Javier

doi:10.1016/j.bios.2010.09.034

Cited by 36 publications

(21 citation statements)

References 29 publications

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“…One example is the disposable DNA biosensor using gold Fe 3 O 4 @AuNPs reported by Loaiza et al [86] for detecting specific hybridization processes. Aβ ab -MNG/SPAuE was used to attach a thiolated 19-mer capture probe and, then, Strept-HRP was bound to the biotinylated target.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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Ultrasensitive biosensing is currently a growing demand that has led to the development of numerous strategies for signal amplification. In this context, the unique properties of magnetic particles; both of nano- and micro-size dimensions; have proved to be promising materials to be coupled with disposable electrodes for the design of cost-effective electrochemical affinity biosensing platforms. This review addresses, through discussion of selected examples, the way that nano- and micro-magnetic particles (MNPs and MMPs; respectively) have contributed significantly to the development of electrochemical affinity biosensors, including immuno-, DNA, aptamer and other affinity modes. Different aspects such as type of magnetic particles, assay formats, detection techniques, sensitivity, applicability and other relevant characteristics are discussed. Research opportunities and future development trends in this field are also considered.

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Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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“…The non‐specific binding between DNA and nanoparticles can be achieved by simple adsorption by non‐covalent interactions. Gold‐coated ferric oxide nanoparticles with an immobilised DNA probe were used as a component of the electrochemical DNA biosensor or as part of different amplification techniques . The authors described a DNA quantification technique based on Fe 3 O 4 /Eu:Gd 2 O 3 core‐shell nanoparticles.…”

Section: Applications Of Magnetic Particles In Dna Separationsmentioning

confidence: 99%

SPE and purification of DNA using magnetic particles

Rittich

Španová

2013

J of Separation Science

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Superparamagnetic particles have been attractive for molecular diagnostics and analytical chemistry applications due to their unique magnetic properties and their ability to interact with various biomolecules of interest. This paper presents a critical overview of magnetic nano- and microparticles used as a solid phase for extraction and purification of DNAs. The mechanisms of DNA binding to the surface of functionalised magnetic particles are described. The most widely used materials including silica supports, organic polymers and other materials, mostly containing magnetite or paramagnetic metallic elements are reviewed. The main application areas of magnetic particles for DNA separation are briefly described.

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“…They promote to develop sensitive and selective biosensing platforms due to their numerous advantages such as easy manipulation and modification, and high surface area 1–5. Thus, many electrochemical biosensor applications developed by using MBs were introduced and the number of MB based studies has increased day‐by‐day 1–3, 6–17. These MB‐based DNA assays have utilized numerous different detection principles, including label free techniques 4, 6, 9, 10 and those utilizing redox‐active labels attached to DNA either chemically 11 or by DNA polymerases 12, enzymatic tags 13, nanoparticles 14 etc.…”

Section: Introductionmentioning

confidence: 99%

“…In the study of Loaiza et al. 3 a disposable magnetic DNA sensor was developed. In order to monitor DNA hybridization, enzymatic amplification strategy based on the coupling of streptavidin‐peroxidase to biotinylated target sequences followed.…”

Section: Introductionmentioning

confidence: 99%

Detection of p53 Gene by Using Genomagnetic Assay Combined with Carbon Nanotube Modified Disposable Sensor Technology

et al. 2015

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Electrochemical monitoring of DNA hybridization related to p53 gene sequence was investigated using genomagnetic assay combined with single walled carbon nanotube (SWCNT) modified pencil graphite electrodes (PGEs). The hybridization was performed either at magnetic beads (MB) surface or in solution. The enhanced guanine signal was obtained using SWCNT‐PGEs compared to one obtained by unmodified PGEs. The selectivity of genomagnetic assay was tested under optimum conditions. The DLs were calculated as 0.88 µM and 0.11 µM for hybridization performed at MB surface and solution, respectively. This selective, practical and cost effective genomagnetic assay combined with SWCNT‐PGEs is reported herein for the first time.

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Gold coated ferric oxide nanoparticles based disposable magnetic genosensors for the detection of DNA hybridization processes

Cited by 36 publications

References 29 publications

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

SPE and purification of DNA using magnetic particles

Detection of p53 Gene by Using Genomagnetic Assay Combined with Carbon Nanotube Modified Disposable Sensor Technology

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