Going wireless: an optical imaging and optogenetics system for use in awake behaving primates

Zaraza, Derek; Chernov, Mykyta M.; Friedman, Robert M.; Yang, Yiyuan; Rogers, John A.; Roe, Anna Wang

doi:10.1117/12.2546289

Cited by 4 publications

(5 citation statements)

References 42 publications

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“…Artificial duras have been used for decades to provide large-scale optical access to the brains of NHPs (Arieli et al 2002, Chernov and Roe 2014, Nassi et al 2015, Seidemann et al 2016, Yazdan-Shahmorad et al 2016, Zaraza et al 2020. Over the years there have been advances in largescale optical imaging and optical stimulation modalities, including the discovery (Boyden et al 2005) and widespread proliferation of optogenetics (Yizhar et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Traditional artificial duras have been used in NHPs for a variety of optical imaging and stimulation modalities including intrinsic optical imaging (Arieli et al 2002, Ruiz et al 2013, Chernov and Roe 2014, Zaraza et al 2020, voltage sensitive dye imaging (Arieli et al 2002, calcium imaging (Seidemann et al 2016), fluorescence imaging (Nassi et al 2015, Yazdan-Shahmorad et al 2016, OCTA (Khateeb et al 2019b), infrared neural stimulation (Chernov and Roe 2014), and optogenetics (Ruiz et al 2013, Nassi et al 2015, Yazdan-Shahmorad et al 2016. With our MMAD we aim to accommodate optical modalities such as these in tandem with ECoG recording.…”

Section: Discussionmentioning

confidence: 99%

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Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

et al. 2021

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Objective. Non-human primates (NHPs) are critical for development of translational neural technologies because of their neurological and neuroanatomical similarities to humans. Large-scale neural interfaces in NHPs with multiple modalities for stimulation and data collection poise us to unveil network-scale dynamics of both healthy and unhealthy neural systems. We aim to develop a large-scale multi-modal interface for NHPs for the purpose of studying large-scale neural phenomena including neural disease, damage, and recovery. Approach. We present a multi-modal artificial dura (MMAD) composed of flexible conductive traces printed into transparent medical grade polymer. Our MMAD provides simultaneous neurophysiological recordings and optical access to large areas of the cortex (∼3 cm2) and is designed to mitigate photo-induced electrical artifacts. The MMAD is the centerpiece of the interfaces we have designed to support electrocorticographic recording and stimulation, cortical imaging, and optogenetic experiments, all at the large-scales afforded by the brains of NHPs. We performed electrical and optical experiments bench-side and in vivo with macaques to validate the utility of our MMAD. Main results. Using our MMAD we present large-scale electrocorticography from sensorimotor cortex of three macaques. Furthermore, we validated surface electrical stimulation in one of our animals. Our bench-side testing showed up to 90% reduction of photo-induced artifacts with our MMAD. The transparency of our MMAD was confirmed both via bench-side testing (87% transmittance) and via in vivo imaging of blood flow from the underlying microvasculature using optical coherence tomography angiography. Significance. Our results indicate that our MMAD supports large-scale electrocorticography, large-scale cortical imaging, and, by extension, large-scale optical stimulation. The MMAD prepares the way for both acute and long-term chronic experiments with complimentary data collection and stimulation modalities. When paired with the complex behaviors and cognitive abilities of NHPs, these assets prepare us to study large-scale neural phenomena including neural disease, damage, and recovery.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

et al. 2021

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show abstract

“…Artificial duras have been used for decades to provide large-scale optical access to the brains of NHPs (Arieli et al ., 2002; Slovin et al ., 2002; Chernov and Roe, 2014; Nassi et al ., 2015; Seidemann et al ., 2016; Yazdan-Shahmorad et al ., 2016; Zaraza et al ., 2020). Over the years there have been advances in large-scale optical imaging and optical stimulation modalities, including the discovery (Boyden et al ., 2005) and widespread proliferation of optogenetics (Yizhar et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Traditional artificial duras have been used in NHPs for a variety of optical imaging and stimulation modalities including intrinsic optical imaging (Arieli et al ., 2002; Ruiz et al ., 2013; Chernov and Roe, 2014; Zaraza et al ., 2020), voltage sensitive dye imaging (Arieli et al ., 2002; Slovin et al ., 2002), calcium imaging (Seidemann et al ., 2016), fluorescence imaging (Nassi et al ., 2015; Yazdan-Shahmorad et al ., 2016), OCTA (Khateeb et al ., 2019b), infrared neural stimulation (Chernov and Roe, 2014), and optogenetics (Ruiz et al ., 2013; Nassi et al ., 2015; Yazdan-Shahmorad et al ., 2016). With our MMAD we aim to accommodate optical modalities such as these in tandem with ECoG recording.…”

Section: Discussionmentioning

confidence: 99%

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

Griggs

Khateeb

Zhou

et al. 2021

Preprint

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Objective. Non-human primates (NHPs) are critical for development of translational neural technologies because of their neurological and neuroanatomical similarities to humans. Large-scale neural interfaces in NHPs with multiple modalities for stimulation and data collection poise us to unveil network-scale dynamics of both healthy and unhealthy neural systems. We aim to develop a large-scale multi-modal interface for NHPs for the purpose of studying large-scale neural phenomena including neural disease, damage, and recovery. Approach. We present a multi-modal artificial dura (MMAD) composed of flexible conductive traces printed into transparent medical grade polymer. Our MMAD provides simultaneous neurophysiological recordings and optical access to large areas of the cortex (~3 cm2) and is designed to mitigate photo-induced electrical artifacts. The MMAD is the centerpiece of the interfaces we have designed to support electrocorticographic recording and stimulation, cortical imaging, and optogenetic experiments, all at the large-scales afforded by the brains of NHPs. We performed electrical and optical experiments bench-side and in vivo with macaques to validate the utility of our MMAD. Main results. Using our MMAD we present large-scale electrocorticography from sensorimotor cortex of three macaques. Furthermore, we validated surface electrical stimulation in one of our animals. Our bench-side testing showed up to 90% reduction of photo-induced artifacts with our MMAD. The transparency of our MMAD was confirmed both via bench-side testing (87% transmittance) and via in vivo imaging of blood flow from the underlying microvasculature using optical coherence tomography angiography. Significance. Our results indicate that our MMAD supports large-scale electrocorticography, large-scale cortical imaging, and, by extension, large-scale optical stimulation. The MMAD prepares the way for both acute and long-term chronic experiments with complimentary data collection and stimulation modalities. When paired with the complex behaviors and cognitive abilities of NHPs, these assets prepare us to study large-scale neural phenomena including neural disease, damage, and recovery.

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“…Optical windows to the NHP brain are often covered with a transparent, silicone-based artificial dura to protect the brain while providing optical access to the cortex (Arieli et al ., 2002; Slovin et al ., 2002; Ruiz et al ., 2013; A Yazdan-Shahmorad et al ., 2016). The artificial dura has been combined with optical stimulation methods such as infrared neural stimulation (Chernov and Roe, 2014) and optogenetics (Ruiz et al ., 2013; Zaraza et al ., 2020). In addition, we have combined electrical recording and stimulation methods using µECoG with optogenetics via an optical window.…”

Section: Introductionmentioning

confidence: 99%

A large-scale optogenetic neurophysiology platform for improving accessibility in NHP behavioral experiments

Griggs,

Bloch,

Stanis

et al. 2024

Preprint

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Optogenetics has been a powerful scientific tool for two decades, yet its integration with non-human primate (NHP) electrophysiology has been limited due to several technical challenges. These include a lack of electrode arrays capable of supporting large-scale and long-term optical access, inaccessible viral vector delivery methods for transfection of large regions of cortex, a paucity of hardware designed for large-scale patterned cortical illumination, and inflexible designs for multi-modal experimentation. To address these gaps, we introduce a highly accessible platform integrating optogenetics and electrophysiology for behavioral and neural modulation with neurophysiological recording in NHPs. We employed this platform in two rhesus macaques and showcased its capability of optogenetically disrupting reaches, while simultaneously monitoring ongoing electrocorticography activity underlying the stimulation-induced behavioral changes. The platform exhibits long-term stability and functionality, thereby facilitating large-scale electrophysiology, optical imaging, and optogenetics over months, which is crucial for translationally relevant multi-modal studies of neurological and neuropsychiatric disorders.Graphical Abstract

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Going wireless: an optical imaging and optogenetics system for use in awake behaving primates

Cited by 4 publications

References 42 publications

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

A large-scale optogenetic neurophysiology platform for improving accessibility in NHP behavioral experiments

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