Goblet cell number in the ileum of rats denervated during suckling and weaning

Hernandes, Luzmarina; Pereira, Lucieni Cristina Marques da Silva; Alvares, Eliana Parisi

doi:10.32604/biocell.2003.27.347

Cited by 13 publications

(1 citation statement)

References 17 publications

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“…Mast cells were quantified per square mm using the software cellSens. Goblet cells were identified by periodic acid-Schiff (PAS) staining (Sigma Aldrich & Leica Microsystems) for detection of mucus-containing cells [16]. The number of goblet cells and epithelial cells were counted in ten randomly selected villi per sample.…”

Section: Histological Analysismentioning

confidence: 99%

Shrimp Extract Exacerbates Allergic Immune Responses in Mice: Implications on Clinical Diagnosis of Shellfish Allergy

Tong,

Li,

Leung

et al. 2024

Clinic Rev Allerg Immunol

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Tropomyosin has been identified as the major cross-reactive shellfish allergen, but recent studies showed the presence of other clinically relevant allergens. This study aims at determining the allergic immune responses of mice sensitized with raw and boiled shrimp extracts in comparison to recombinant tropomyosin (rTM). Female Balb/c mice were intragastrically sensitized and challenged with raw, boiled shrimp or rTM. Systemic, cellular and humoral allergic responses were compared, while allergenicity of the extracts was also compared by skin prick test (SPT) and immunoblot on shrimp allergic subjects. We showed that rTM and shrimp extracts induced IgE- and Th2-mediated allergic responses in mice, distinguished by remarkable intestinal inflammation in small intestine across all regimens. Notably, boiled shrimp extract exhibited the highest sensitization rate (73.7% of mice developed positive TM-specific IgE response) when compared with raw extract (47.8%) and rTM (34.8%). Mice sensitized with boiled extract manifested the highest allergen-specific IgE and Th2 cytokine responses than the others. Immunoblot results indicated that tropomyosin remained the major allergen in extract-based sensitization and had stronger allergenicity in a heat-treated form comparing to untreated TM, which was in line with the SPT results that boiled extract induced larger wheal size in patients. Hemocyanin and glycogen phosphorylase were also identified as minor allergens associated with manifestation of shrimp allergy. This study shows that boiled extract enhanced sensitization and Th2 responses in agreement with the higher allergenicity of heat-treated TM. This study thus presents three shrimp allergy murine models suitable for mechanistic and intervention studies, and in vivo evidence implies higher effectiveness of boiled extract for the clinical diagnosis of shellfish allergy.

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Section: Histological Analysismentioning

confidence: 99%

Shrimp Extract Exacerbates Allergic Immune Responses in Mice: Implications on Clinical Diagnosis of Shellfish Allergy

Tong,

Li,

Leung

et al. 2024

Clinic Rev Allerg Immunol

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Myenteric denervation differentially reduces enteroendocrine serotonin cell population in rats during postnatal development

Hernandes

Fernandes²,

Pereira

et al. 2006

J Mol Hist

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The enteric nervous and enteroendocrine systems regulate different processes in the small intestine. Ablation of myenteric plexus with benzalkonium chloride (BAC) stimulates epithelial cell proliferation, whereas endocrine serotonin cells may inhibit the process. To evaluate the connection between the systems and the influence of myenteric plexus on serotoninergic cells in rats during postnatal development, the ileal plexus was partially removed with BAC. Rats were treated at 13 or 21 days and sacrificed after 15 days. The cell bodies of myenteric neurons were stained by beta NADH-diaphorase to detect the extension of denervation. The number of enteroendocrine cells in the ileum was estimated in crypts and villi in paraffin sections immunostained for serotonin. The number of neurons was reduced by 27.6 and 45% in rats treated on the 13th and 21st days, respectively. We tried to establish a correlation of denervation and the serotonin population according to the age of treatment. We observed a reduction of immunolabelled cells in the crypts of rats treated at 13 days, whereas this effect was seen in the villi of rats denervated at 21 days. These results suggest that the enteric nervous system might control the enteroendocrine cell population and this complex mechanism could be correlated to changes in cell proliferation.

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Epigenetic Control of Animal Development

Cabej

2013

Building the Most Complex Structure on Earth

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Goblet cell number in the ileum of rats denervated during suckling and weaning

Cited by 13 publications

References 17 publications

Shrimp Extract Exacerbates Allergic Immune Responses in Mice: Implications on Clinical Diagnosis of Shellfish Allergy

Shrimp Extract Exacerbates Allergic Immune Responses in Mice: Implications on Clinical Diagnosis of Shellfish Allergy

Myenteric denervation differentially reduces enteroendocrine serotonin cell population in rats during postnatal development

Epigenetic Control of Animal Development

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