Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro

Martins, Gabrielle Rosemblit; Marinho, Rebeca Cavalcante; Bezerra, Rosivaldo Quirino; Alves, Antoniel de O.; Câmara, Lilia Maria Carneiro; Albuquerque-Pinto, Luiz C.; Teixeira, Maria Fátima da Silva

doi:10.1016/j.bjm.2016.11.002

Cited by 4 publications

(2 citation statements)

References 38 publications

(38 reference statements)

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“…In the present study, results from nPCR and Western Blot demonstrated the occurrence of SRLV-positive neonates from mothers infected by these viral agents. These results were confirmed via cell culture, and observation of syncytia and cell destruction, which are some of the main cytopathic effects characteristic of SRLV evidenced in the literature [20,26,32,33].…”

Section: Discussionsupporting

confidence: 74%

Vertical transmissibility of small ruminant lentivirus

et al. 2020

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This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.

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Section: Discussionsupporting

confidence: 74%

Vertical transmissibility of small ruminant lentivirus

et al. 2020

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“…Hence, the data obtained in this study performed with co-culture of SC of colostrum and SC of milk and OTE cells after use of ethanolic extracts of A. indica and M. azedarach may reveal a possible antiviral activity against CLV. This fact was observed mainly in the organic fraction of ethyl acetate, with an action time of 90 min, with marked inhibition of syncytium formation and cell destruction, which are considered the main characteristic cytopathic effects of CVL elucidated even in previous research studies, where the adoption of a qualitative evaluation through the visualization of their occurrence levels in cultured cells is standard 19,[42][43][44][45][46] .…”

Section: Discussionmentioning

confidence: 95%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.

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