GOAT rs10096097 and CREB1 rs6740584 single nucleotide polymorphisms are associated with type 2 diabetes mellitus in Egyptians

Abstract

Diabetes mellitus (DM) is a chronic disorder that affects nearly half a billion people around the world and causes millions of deaths annually. Treatment of diabetes or related complications represents an economic burden not only for developing countries but also for the developed ones. Hence, new efficient therapeutic and preventive strategies and screening tools are necessary. The current work aimed to assess the potential association of single nucleotide polymorphisms (SNPs) in ghrelin O‐acyltransferase (GOAT) rs10096097, cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) rs6740584, and v‐maf musculoaponeurotic fibrosarcoma oncogene homolog A (MafA) rs62521874 genes with type 2 DM susceptibility in Egyptians. A total of 96 patients with type 2 DM along with 72 healthy individuals participated in this study. Genotyping was executed via real‐time polymerase chain reaction (PCR), and the serum protein levels of GOAT, CREB, and MafA were measured by enzyme‐linked immunosorbent assay (ELISA). Genotyping revealed a significant association of GOAT rs10096097 and CREB1 rs6740584 SNPs with type 2 diabetes risk, with significantly higher GOAT rs10096097 G allele and CREB1 rs6740584 T allele frequencies in diabetic patients than in controls. However, insignificant association was identified between the MafA rs62521874 SNP and diabetes in the examined sample of the Egyptian residents. Serum GOAT, CREB1, and MafA protein levels did not vary significantly between diabetic and control individuals. Yet, significant variation in serum GOAT and CREB1 levels was detected between CREB1 rs6740584 genotypes within the diabetic group, with CT and TT genotype carriers showing higher levels than AA genotype patients. GOAT rs10096097 and CREB1 rs6740584, but not MafA rs62521874, SNPs are associated with type 2 diabetes risk in the studied Egyptians.

Identification of key genes and signaling pathway in the pathogenesis of Huntington's disease via bioinformatics and next generation sequencing data analysis

“…Previous studies have reported that EPAS1 [486], HOXA1 [735], TGM2 [449], CCND2 [500], EGFR (epidermal growth factor receptor) [276], NEDD4L [496], DCLK1 [504], hsa-mir-363-3p [738], hsa-mir-21-5p [743], SMAD4 [744], ATF3 [745], TBX5 [746], SREBF1 [747], SMARCA4 [748] and CREB1 [749] were regulated in cardiovascular diseases. Studies reported that ERBB3 [255], TGM2 [223], CCND2 [274], EGFR (epidermal growth factor receptor) [276], hsa-mir-21-5p [750], ATF3 [751], SREBF1 [752], ARNT (aryl hydrocarbon receptor nuclear translocator) [753] and CREB1 [754] play important roles in diabetes mellitus. ERBB3 [340], TGM2 [319], EGFR (epidermal growth factor receptor) [367], DCLK1 [366], SMAD4 [755], SREBF1 [756] and CREB1 [757] participated in the regulation of schizophrenia.…”