Go with the flow: mechanisms driving water transport during vegetative growth and fruiting

Herman, Koen C.; Bleichrodt, Robert-Jan

doi:10.1016/j.fbr.2021.10.002

Cited by 14 publications

(7 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rather, they try to maintain the functionality of their cells (see below) with minimal turgor, and leave the increase in turgor pressure for later, when external conditions have stabilized. Turgor pressure, rather, is not important in its absolute value (with the exception of apical and some specialized cells), what is more important is its gradient along the length of the hyphae, which ensures mass flow transport, and this does not require high absolute pressure values (Lew, 2019; Herman, Bleichrodt, 2022).…”

Section: Discussionmentioning

confidence: 99%

Instantaneous change in hyphal diameter in basidiomycete fungi

Mazheika,

Voronko,

Kolomiets

et al. 2024

Preprint

View full text Add to dashboard Cite

Under certain conditions, fungi are capable to fast (down to seconds) change the size of their cells. For example, it is known that the cells of many species of yeasts under hyperosmotic conditions instantly and reversibly shrink entirely, without plasmolysis, with a decrease in volume of up to 40% or more. There is limited data that filamentous fungi can also instantly change the diameter of their unspecialized hyphae. This property is fundamental and is a powerful tool for survival and preservation of the physiological functionality of the fungus in sharply changing external conditions, but requires detailed study. In this work, which is a large-scale (more than 50,000 cells measured) light microscopic study, the ability of three unrelated basidiomycete species to fast change the diameter of their hyphae under the influence of various factors was analyzed. It was found, for example, that all three fungi respond similarly to moderate hyperosmotic shock and shrink by an average of 12-14% in diameter, but inhibitors of actin assembly can cause both expansion and shrinkage of hyphae, or have no effect on a fungus. Combining the current results with those previously obtained in the study of macroinvagination systems of the fungal plasma membrane, different approaches for basidiomycetes to respond to sudden stress changes were proposed. The curtain model was also expanded, describing a common rapid response mechanism. The mechanism includes elastic properties of the cell wall, adhesion of the plasma membrane to it, regulation of turgor, the actin cables system that regulate the tension of the plasma membrane and cell size, and the complex system of macroinvaginations of the plasma membrane, ensuring the constant integrity and functionality of the latter.

show abstract

Section: Discussionmentioning

confidence: 99%

Instantaneous change in hyphal diameter in basidiomycete fungi

Mazheika,

Voronko,

Kolomiets

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…It exudes enzymes (which promote decomposition) onto the substrate surface, and the nutrients that become available are absorbed back into the mycelial network. This mycelial network will continue to expand until it no longer has a fresh nutrient source to colonize or until it encounters another competitive organism, such as a contaminant in the culture [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

First Record of the Edible Mushroom Lepista sordida in Western Algerian Forest: Nutritional Value and Physicochemical Parameters of Mycelial Culture

Alim,

Sidhoum,

Dib

2023

JoF

View full text Add to dashboard Cite

The exploration of the western forests of Algeria led to the remarkable discovery of the first occurrence of Lepista sordida, an edible wild mushroom of significant culinary importance for the local community, traditionally consumed in its natural state. This discovery was made possible through the use of various methods, including macroscopic observations (revealing a violet color) as well as microscopic observations conducted using scanning electron microscopy (SEM), revealing a cylindrical shape with distinct contours. Additionally, molecular analyses were conducted. Genomic DNA was extracted from the mycelium, followed by DNA amplification using specific primers targeting the internal transcribed spacer region (ITS1 and ITS2). After PCR reactions and sequencing of the obtained amplicons, the nucleotide sequences of the mycelium were submitted to the GenBank database of NCBI with the assigned accession number: MZ928450.1. These sequences were subsequently used to construct the phylogenetic tree. Furthermore, an in-depth study of physicochemical parameters was undertaken to determine the optimal conditions for cultivating the mycelium of this edible wild mushroom, including pH, temperature, relative humidity, and light. Different temperatures were examined: 20, 25, 30, 35, 40, and 45 °C. The effect of pH on mycelium growth was studied using a PDA agar medium with buffered values of 4, 5, 5.6, 6, 7, and 8. Similarly, six levels of relative humidity were tested: 14, 50, 74, 80, 95, and 100%. A study on the impact of light on mycelium growth was conducted by exposing Petri dishes inoculated with PDA to a light intensity of 500 lux for 5, 10, 15, 20, and 24 h. The results clearly demonstrated that variations in these different physicochemical parameters significantly influenced mycelium growth. For the Lepista sordida strain, growth was favored at pH levels of 4, 5, 6, and 6, with no growth observed at pH 7 and 8. The optimal temperature range for mycelium growth of Lepista sordida was 20–25 °C, while no growth was observed at 30, 35, 40, and 45 °C. Relative humidity levels of 74, 80, and 95% showed no significant differences. Optimization of mycelium growth and primordia production in Lepista sordida were successfully achieved. Optimal conditions for the primordia phase were identified as 25 °C, with humidity ranging from 90 to 95%. A nutritional analysis of fresh sporophores was conducted using established analytical methods. Notably, the nutritional composition of Lepista sordida sporophores exhibited high significance for the following parameters: moisture content (67.23 ± 1.90%), ash content (9.35 ± 0.66%), fat content (3.25 ± 0.24%), protein content (17.22 ± 0.38%), and carbohydrate content (63.83 ± 1.23%).

show abstract

“…One important environmental factor with major effects on fungal activity is soil water availability expressed by the soil water potential. The soil water potential represents the energy with which water is retained in the soil (Robert & Chenu, 1995; Walker & White, 2017), and water can move from areas with high to low water potential (Herman & Bleichrodt, 2022). The experiment was designed to examine the capacity of fungi to improve the water potential in soil based on the significant effects of fungal inoculation on soil aggregation and hydrophobic properties.…”

Section: Methodsmentioning

confidence: 99%

“…Fungi, for instance, acidify the soil by secreting organic acids (Liaud et al, 2014). These organic acids will dissolve nutrients thereby releasing ions (Haynes & Swift, 1986) and lowering the water potential of the soil (Herman & Bleichrodt, 2022), but also enhancing the feeding of the plant roots. Fungal respiration will also result in water loss, but this is expected to play a relatively minor role in the total soil water budget (Flegg, 1974).…”

Section: Effect Of Fungal Strain Inoculation On Soil Water Potentialmentioning

confidence: 99%

Enhancement of soil aggregation and physical properties through fungal amendments under varying moisture conditions

Angulo,

Bleichrodt,

Dijksterhuis

et al. 2024

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Soil structure and aggregation are crucial for soil functionality, particularly under drought conditions. Saprobic soil fungi, known for their resilience in low moisture conditions, are recognized for their influence on soil aggregate dynamics. In this study, we explored the potential of fungal amendments to enhance soil aggregation and hydrological properties across different moisture regimes. We used a selection of 29 fungal isolates, recovered from soils treated under drought conditions and varying in colony density and growth rate, for single‐strain inoculation into sterilized soil microcosms under either low or high moisture (≤−0.96 and –0.03 MPa, respectively). After 8 weeks, we assessed soil aggregate formation and stability, along with soil properties such as soil water content, water hydrophobicity, sorptivity, total fungal biomass and water potential. Our findings indicate that fungal inoculation altered soil hydrological properties and improved soil aggregation, with effects varying based on the fungal strains and soil moisture levels. We found a positive correlation between fungal biomass and enhanced soil aggregate formation and stabilization, achieved by connecting soil particles via hyphae and modifying soil aggregate sorptivity. The improvement in soil water potential was observed only when the initial moisture level was not critical for fungal activity. Overall, our results highlight the potential of using fungal inoculation to improve the structure of agricultural soil under drought conditions, thereby introducing new possibilities for soil management in the context of climate change.

show abstract

Go with the flow: mechanisms driving water transport during vegetative growth and fruiting

Cited by 14 publications

References 97 publications

Instantaneous change in hyphal diameter in basidiomycete fungi

Instantaneous change in hyphal diameter in basidiomycete fungi

First Record of the Edible Mushroom Lepista sordida in Western Algerian Forest: Nutritional Value and Physicochemical Parameters of Mycelial Culture

Enhancement of soil aggregation and physical properties through fungal amendments under varying moisture conditions

Contact Info

Product

Resources

About