GntR family regulator SCO6256 is involved in antibiotic production and conditionally regulates the transcription of myo-inositol catabolic genes in Streptomyces coelicolor A3(2)

Yu, Lingjun; Gao, Wenyan; Li, Shuxian; Pan, Yuanyuan; Liu, Gang

doi:10.1099/mic.0.000235

Cited by 6 publications

(2 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S3a), indicating that ROS played an important role in improving the expression of ACT genes. As pathway-specific regulators are generally considered to have a most direct effect on antibiotic production via transcriptional activation of the relevant biosynthetic genes, the pathway-specific regulatory protein was revealed to be ActII-ORF4 in the biosynthesis of ACT 34,35 . Factors that influence the production of ACT are mostly affected by regulation of transcription or translation of actII-ORF4 (SCO5085) 36 .…”

Section: Resultsmentioning

confidence: 99%

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

Liu

Tang

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

In this research, antibiotic-producing bacteria, Streptomyces coelicolor ( S. coelicolor ) M145, was exposed to copper oxide (CuO) particles to investigate the effects of nano-particles (NPs) on antibiotic production. Results showed that a higher yield of antibiotics was obtained with smaller particle sizes of CuO NPs. When exposed to 10 mg/L of 40 nm CuO NPs, the maximum amount of actinorhodin (ACT) obtained was 2.6 mg/L after 144 h, which was 2.0-fold greater than that of control. However, the process was inhibited when the concentration of CuO NPs was increased to higher than 20 mg/L. Transcriptome analysis showed that all the genes involved in the ACT cluster were significantly up-regulated after exposure to 10 mg/L NPs, which could be the direct cause of the increase of ACT production. Additionally, some genes related to the generation of acetyl-coA were up-regulated. In this way, CuO NPs led to an increase of secondary metabolites. The mechanism related to these changes indicated that nano-particle‒induced ROS and Cu 2+ played synergetic roles in promoting ACT biosynthesis. This is a first report suggesting that CuO NPs had a significant effect on antibiotic production, which will be helpful in understanding the mechanism of antibiotic production in nature.

show abstract

Section: Resultsmentioning

confidence: 99%

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

Liu

Tang

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…S. coelicolor A3(2) genome contains 56 genes for GntR-like proteins representing five subfamilies. Eleven of them were described experimentally (Hoskisson et al, 2006; Rigali et al, 2008; Horbal et al, 2013; Persson et al, 2013; Cen et al, 2016; Yu et al, 2015, 2016; Tsypik et al, 2016, 2017), but the low molecular ligands have been identified in only two cases. Glucosamine 6-phosphate is the effector molecule bound by a master regulator DasR which links nutrient stress to antibiotic production (Rigali et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A GntR-Like Transcription Factor HypR Regulates Expression of Genes Associated With L-Hydroxyproline Utilization in Streptomyces coelicolor A3(2)

et al. 2019

View full text Add to dashboard Cite

Bacteria from the genus Streptomyces have been long exploited as the most prolific producers of antibiotics, other secondary metabolites and enzymes. They are important members of soil microbial communities that can adapt to changing conditions thank to the fine regulation of gene expression in response to environmental signals. Streptomyces coelicolor A3(2) is a model organism for molecular studies with the most deeply recognized interactions within the complex metabolic and regulatory network. However, details about molecular signals recognized by specialized regulatory proteins as well as their direct targets are often missing. We describe here a zinc-binding protein HypR (SCO6294) which belongs to FadR subfamily of GntR-like regulators. The DNA sequence 5′-TACAATGTCAC-3′ recognized by the HypR protein in its own promoter region was identified by DNase I footprinting. Binding of six DNA fragments containing similar sequences located in other promoter regions were confirmed by the electrophoretic mobility shift assay (EMSA). The sequences of 7 in vitro -determined binding sites were assembled to generate a logo of the HypR binding motif, 5′-CTNTGC(A/C)ATGTCAC-3′. Comparison of luciferase reporter genes expression under the control of cloned promoter regions in S. coelicolor A3(2) wild type and deletion mutant strains revealed, that the HypR protein acts as a repressor of its target genes. Genes belonging to the regulon of HypR code for enzymes putatively involved in collagen degradation and utilization of L-hydroxyproline (L-Hyp) as concluded from predicted structure and conserved domains. Their transcription is induced in the wild type strain by the addition of L-Hyp to the culture medium. Moreover, knockout of one of the genes from the predicted L-Hyp utilization operon abolished the ability of the strain to grow on L-Hyp as a sole source of carbon. To our knowledge, this work is the first indication of the existence of the pathway of L-hydroxyproline catabolism in Streptomycetes.

show abstract

Mechanism of salinomycin overproduction in Streptomyces albus as revealed by comparative functional genomics

Zhang

Bai

2017

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The anticoccidial salinomycin is a polyketide produced by Streptomyces albus, and the high-yield strain BK 3-25 produces 18.0 g/L salinomycin under lab condition. In order to elucidate the overproduction mechanism, the genome of BK 3-25 was fully sequenced and compared with the wild-type DSM 41398. Strain BK 3-25 has a 75-kb large deletion, containing type-I polyketide gene cluster PKS-9, and 60 additional InDels and SNVs affecting 55 CDSs, including a 1-bp deletion in type-I PKS gene cluster PKS-6. Subsequently, individual or combined deletions of the 75-kb region and PKS-6 in the wild-type resulted in improved salinomycin yields from 2.60 to 5.20, 6.90, and 9.50 g/L (53% of BK 3-25), respectively, suggesting a redirected flux of polyketide precursors to salinomycin biosynthesis. Moreover, due to the much higher transcription of salinomycin biosynthetic genes (sln) in the high-yield BK 3-25 than in the wild-type, 13 putative regulatory genes among the 55 CDSs were individually inactivated and 7 were proved to be negatively involved in the transcription of sln genes. Combined deletions of two major negative regulatory genes SLNWT_3357 and SLNWT_7015 caused further improved transcription of sln genes as well as the yield, from 2.60 to 7.30 g/L (40% of BK 3-25). Therefore, the comparative genomics approach combined with functional experiments identified that the multiple deletions and mutations of competing gene clusters and negative regulatory genes are crucial for salinomycin overproduction, setting an example for rational titer improvement of other polyketide natural products.

show abstract

GntR family regulator SCO6256 is involved in antibiotic production and conditionally regulates the transcription of myo-inositol catabolic genes in Streptomyces coelicolor A3(2)

Cited by 6 publications

References 49 publications

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

A GntR-Like Transcription Factor HypR Regulates Expression of Genes Associated With L-Hydroxyproline Utilization in Streptomyces coelicolor A3(2)

Mechanism of salinomycin overproduction in Streptomyces albus as revealed by comparative functional genomics

Contact Info

Product

Resources

About