GnRH Molecular Variants in the Brain and Pituitary Gland of Pejerrey, Odontesthes bonariensis (Atheriniformes). Immunological and Chromatographic Evidence for the Presence of a Novel Molecular Variant

Stefano, Andrea V.; Canosa, Luis Fabián; D'Eramo, José L.; Fridman, Osvaldo; Affanni, Jorge M.; Somoza, Gustavo M.

doi:10.1016/s0742-8413(97)00135-7

Cited by 11 publications

(8 citation statements)

References 40 publications

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“…The primary structure of the novel form was determined to be: pGlu-His-Trp-Ser-Phe-Gly-Leu-Ser-Pro-Gly-NH 2 . Previous indirect evidence based on chromatographic and immunological data had suggested that three forms might be present (6). The novel form in the present paper is termed pejerrey (pj)GnRH in keeping with the tradition that GnRH peptides are named for the species in which they are first described.…”

Section: Discussionsupporting

confidence: 53%

“…Although the neuroanatomical distribution of pjGnRH has not been clearly demonstrated as yet, it can be considered the releaser of pituitary hormones because it is the only GnRH form found in pituitary extracts of pejerrey (6). Our evidence shows that the newly described pjGnRH form is present in pejerrey brain together with cGnRH-II and sGnRH.…”

Section: Fig 5 Comparison Of the Known Gnrh Structures With Mgnrhmentioning

confidence: 62%

“…Also, the form of GnRH found in the pejerrey, Odontesthes bonariensis, was reported to coexist with sGnRH and cGnRH-II based on RP-HPLC/RIA studies (6). The pejerrey fish was originally found only in the inland waters of the Province of Buenos Aires in Argentina.…”

Section: G Nrh Is a Key Neurohormone For Reproduction In Allmentioning

confidence: 99%

“…Both herring and pejerrey express cGnRH-II, sGnRH and a third form that is the dominant form in the pituitary. The data suggest that hrGnRH and pjGnRH are the GnRH peptides expressed by preoptic neurons that send axons to the pituitary gland (3,6). However, the origin of hrGnRH is not yet clear.…”

Section: Fig 5 Comparison Of the Known Gnrh Structures With Mgnrhmentioning

confidence: 99%

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Primary Structure of a Novel Gonadotropin-Releasing Hormone in the Brain of a Teleost, Pejerrey

Montaner¹

2001

Endocrinology

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The neuropeptide GnRH is the major regulator of reproduction in vertebrates acting as a first signal from the hypothalamus to pituitary gonadotropes. Three GnRH molecular variants were detected in the brain of a fish, pejerrey (Odontesthes bonariensis), using chromatographic and immunological methods. The present study shows that one form is identical to chicken GnRH-II (sequence analysis and mass spectrometry) and the second one is immunologically and chromatographically similar to salmon GnRH. The third form was proven to be a novel form of GnRH by isolating the peptide from the brain and determining its primary structure by chemical sequencing and mass spectrometry. The sequence of the novel pejerrey GnRH is pGlu-HisTrp-Ser-Phe-Gly-Leu-Ser-Pro-Gly-NH 2 , which is different from the known forms of the vertebrate and protochordate GnRH family. The new form of GnRH is biologically active in releasing gonadotropin and GH from pituitary cells in an in vitro assay. (Endocrinology 142:

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Section: Discussionsupporting

confidence: 53%

Section: Fig 5 Comparison Of the Known Gnrh Structures With Mgnrhmentioning

confidence: 62%

Section: G Nrh Is a Key Neurohormone For Reproduction In Allmentioning

confidence: 99%

Section: Fig 5 Comparison Of the Known Gnrh Structures With Mgnrhmentioning

confidence: 99%

See 2 more Smart Citations

Primary Structure of a Novel Gonadotropin-Releasing Hormone in the Brain of a Teleost, Pejerrey

Montaner¹

2001

Endocrinology

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“…Postprandial (+3 and +1 h) preproghrelin mRNA expression and serum ghrelin levels in fed and unfed fish were compared using an independent t test. The slopes of the serial dilution of goldfish serum and the synthetic standards were compared by ANOVA using a computer program [28]. Significance was considered at p < 0.05.…”

Section: Experiments Design Materials and Methodsmentioning

confidence: 99%

Orexigenic Actions of Ghrelin in Goldfish: Feeding-Induced Changes in Brain and Gut mRNA Expression and Serum Levels, and Responses to Central and Peripheral Injections

Unniappan

Canosa

Peter³

2004

Neuroendocrinology

183

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In this study, we examined (i) the preprandial, postprandial and starvation-induced changes in the preproghrelin mRNA expression and serum ghrelin levels, and (ii) the effects of intracerebroventricular and intraperitoneal administration of ghrelin on food intake in goldfish. Slot blot analysis revealed a significant postprandial decrease in preproghrelin mRNA expression in the hypothalamus (1 and 3 h after feeding) and gut (3 h after feeding). A similar postprandial decrease (1 and 3 h after feeding) in serum ghrelin levels was also detected. In the fish that were unfed at the regular feeding time, the hypothalamic preproghrelin mRNA expression and the serum ghrelin levels remained unchanged, while the preproghrelin mRNA expression in the gut decreased 3 h after the regular feeding time. Starvation increased preproghrelin mRNA expression in the hypothalamus and gut on the 7th day. Serum ghrelin levels were significantly elevated on days 3 and 5 of starvation. Intracerebroventricular injections of n-octanoylated ghrelin-like peptides (gGRL_[1–12]) (10 ng/g body weight) and human ghrelin (1 and 10 ng/g body weight) and intraperitoneal injections of n-octanoylated gGRL_[1–12] (10 ng/g body weight), gGRL_[1–19] (100 ng/g body weight) and human ghrelin (10 and 100 ng/g body weight) stimulated food intake in goldfish. The patterns of synthesis, secretion and actions indicate that ghrelin is an orexigen in goldfish.

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Gonadotropin-Releasing Hormone (GnRH) Variants in a Lizard Brain: Is Mammalian GnRH Being Expressed?

Montaner

González

Paz

et al. 2000

General and Comparative Endocrinology

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GnRH Molecular Variants in the Brain and Pituitary Gland of Pejerrey, Odontesthes bonariensis (Atheriniformes). Immunological and Chromatographic Evidence for the Presence of a Novel Molecular Variant

Cited by 11 publications

References 40 publications

Primary Structure of a Novel Gonadotropin-Releasing Hormone in the Brain of a Teleost, Pejerrey

Primary Structure of a Novel Gonadotropin-Releasing Hormone in the Brain of a Teleost, Pejerrey

Orexigenic Actions of Ghrelin in Goldfish: Feeding-Induced Changes in Brain and Gut mRNA Expression and Serum Levels, and Responses to Central and Peripheral Injections

Gonadotropin-Releasing Hormone (GnRH) Variants in a Lizard Brain: Is Mammalian GnRH Being Expressed?

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