GnRH-agonist implants suppress reproductive function and affects ovarian LHR and FSHR expression in prepubertal female cats

Mehl, N.S.; Srisuwatanasagul, Sayamon; Swangchan-Uthai, Theerawat; Sirivaidyapong, S.; Khalid, M.

doi:10.1016/j.theriogenology.2016.09.003

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…GnRH agonist can significantly inhibit LH expression in rat ovarian GCs (Litichever et al, 2009). GnRH inhibited ovarian LHR expression in cats in puberty (Mehl et al, 2017). These findings suggest that ovarian GnRH plays a role by downregulating LH and LHR.…”

Section: Discussionmentioning

confidence: 74%

“…Khalaf et al (2010) found that the ovarian granulosa cells (GCs) with the treatment of GnRH antagonist could significantly reduce the content of estradiol (E2). GnRH agonists can affect the expression of gonadotropin receptors in the ovary in prepubertal female cats (Mehl et al, 2017). GnRH and its receptors are expressed in many nonhypothalamic reproductive tissues, such as the ovary, uterus, and fallopian tube (La et al, 2010;Peng et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Expression and functional analysis of GnRH at the onset of puberty in sheep

Zhang

Wang²,

Li³

et al. 2022

Arch. Anim. Breed.

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Abstract. Gonadotropin-releasing hormone (GnRH) is a key factor at the onset of puberty. This decapeptide has been found in mammalian ovaries, but its regulatory mechanism in the ovary of sheep at the onset of puberty is not clear. This study investigated the coding sequence (CDS) of the GnRH gene in the ovary of Duolang sheep and the expression of GnRH mRNA in different tissues at the onset of puberty, and analyzed the effect of GnRH on ovarian granulosa cells (GCs) of Duolang sheep. The results showed that the GnRH CDS of sheep was cloned, the full length of the GnRH CDS in sheep ovary was 279 bp, and the nucleotide sequence was completely homologous to that in the hypothalamus. The expression of GnRH mRNA was highest in the hypothalamus and ovary. The expression of related hormones and receptors in GCs of Duolang sheep treated with different concentrations of GnRH for 24 h was affected. GnRH significantly inhibited LH synthesis and LHR expression in GCs. Low concentration (100 ng mL−1) had the most obvious therapeutic effect on follicle-stimulating hormone (FSH) and FSHR. Higher concentration (250 ng mL−1) significantly promoted estradiol and ERβ mRNA. These findings provide strong evidence that ovarian GnRH is an important regulatory factor at the onset of puberty in sheep.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Expression and functional analysis of GnRH at the onset of puberty in sheep

Zhang

Wang²,

Li³

et al. 2022

Arch. Anim. Breed.

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“…In this study, we demonstrated that the expressions of hormone-related genes were altered in the broody ovary. Follicle stimulating hormone receptor (FSHR) [22], growth hormone inducible transmembrane protein (GHITM) [23] and NPY [13] genes, which encode important receptor or signaling factors in intracellular signaling of hormones regulating folliculogenesis, were decreased in broody geese. Phospholipase C beta 1 (PLCB1), as an important enzyme for oocyte activation, promote the release of prolactin by mediating GnRH [24].…”

Section: Discussionmentioning

confidence: 99%

High-throughput small RNA and transcriptome sequencing reveal reproduction-related microRNAs and mRNA in ovary of Geese in Counter-Season Production

Ran

Yin

et al. 2019

Preprint

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Background Broodiness is a phenomenon that occurs in most avian species and significantly reduces productivity. Several genes are known to play an important role in regulating the progress of reproduction, but the molecular regulation mechanism of broodiness remains unclear. In the current study, via high throughput sequencing, we identified and explored the differentially expressed miRNAs and mRNAs involved in ovarian atrophy. Results We identified a total of 901 mRNAs and 50 miRNAs that were differentially expressed in egg-laying and atrophic ovaries. Among them, numerous DEGs transcripts and target genes for miRNAs were significantly enriched in reproductive processes, cell proliferation, and apoptosis pathways. In addition, a miRNA- gene-pathway network was constructed by considering target relationships and correlation of the expression levels between ovary development-related genes and miRNAs. Conclusions We discovered mRNA and miRNAs transcripts that are candidate regulators of ovary development in broody geese. Our findings expanded our understanding of the functional of miRNAs in ovarian atrophy and demonstrated that RNA-Seq is a powerful tool for examining the molecular mechanism in regulating broodiness.

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“…Consequently, GnRH agonists may either stimulate estrus or effectively sterilize the dog, depending on the expected effect, i.e., FU effect or GnRH desensitization. This double mechanism of action explains why suprelorin has been extensively used over the years, in a wide range of clinical applications and in many species: male sterilization in dogs (4,(8)(9)(10)(11)(12), oestrus induction in female dogs (13)(14)(15)(16), oestrus inhibition in female cats (17)(18)(19)(20), contraception and prevention of adrenal disease in male ferrets (21)(22)(23) as well as females (24), estrus supression in ferrets (25), delaying puberty in male dogs (26,27) and cats (28,29), management of benign prostatic hyperplasia (9,30), and treatment of sex hormone-related behavioral problems (31)(32)(33) in dogs.…”

Section: Introductionmentioning

confidence: 99%

Double-Blind, Placebo-Controlled Trial of Cyproterone Acetate to Prevent Flare-Up Effect on Dogs Implanted With Deslorelin

Masson

Medam²,

Raibon

et al. 2021

Front. Vet. Sci.

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Deslorelin slow-released implants are registered in Europe for the reversible suppression of fertility in male dogs. After administration, a time-limited increase in sex hormones concentration and related behavioral problems may be observed. The aim of this work was to assess whether cyproterone acetate, a synthetic progestogen, can prevent this flare-up effect. Eighteen privately-owned entire male dogs were enrolled in this double-blind, placebo-controlled, randomized clinical trial. All subjects received a 4.7 mg deslorelin implant by SC route and 1–3 capsules containing either cyproterone acetate 2 mg/kg (N = 9) or a placebo (N = 9), by oral route BID for 14 days, depending on the dog's weight. The dogs were followed for 28 days. An increase in the blood testosterone concentration was observed in respectively 9/9 and 7/9 dogs of the control and cyproterone groups (p = 0.47). However, a worsening of the sex hormone related problems (i.e., urinary marking, mounting, aggressiveness toward other dogs and/or escape) was only observed in the placebo group, in 56 or 66% of the dogs as measured by respectively the veterinarian and the owners. Our study suggests that cyproterone acetate is effective and safe to supress the deslorelin induced behavioral flare-up effect, but not the rise in testosterone.

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GnRH-agonist implants suppress reproductive function and affects ovarian LHR and FSHR expression in prepubertal female cats

Cited by 10 publications

References 24 publications

Expression and functional analysis of GnRH at the onset of puberty in sheep

Expression and functional analysis of GnRH at the onset of puberty in sheep

High-throughput small RNA and transcriptome sequencing reveal reproduction-related microRNAs and mRNA in ovary of Geese in Counter-Season Production

Double-Blind, Placebo-Controlled Trial of Cyproterone Acetate to Prevent Flare-Up Effect on Dogs Implanted With Deslorelin

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