GNPs-CS/KGM as Hemostatic First Aid Wound Dressing with Antibiotic Effect: In Vitro and In Vivo Study

Fan, Li; Cheng, Chong; Qiao, Youbei; Li, Fēi; Li, Wei; Wu, Hong; Ren, Bo

doi:10.1371/journal.pone.0066890

Cited by 16 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, wound healing, using this product, can be attributed both to the bactericidal effect and the favoring of wound healing by NO (24) . Other authors were also interested in studying the bactericidal action, according to study A10, which analyzed products as chitosan/glucomannan konjac (GNP-CS/KGM) film, incorporated with gentamicin poly (dex-GMA/AAC) nanoparticles, which identified good antibiotic capacity, as well as a hemostatic effect, shortening the bleeding time and significantly reducing the volume in relation to the control group (25) . Study A11 evaluated the effect of calcium nanoparticles (CNP) on wound healing in rats in topical and intravascular use.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Utilização de nanopartículas no tratamento de feridas: revisão sistemática

Silva

Aguiar

Rodrigues

et al. 2018

Rev. esc. enferm. USP

View full text Add to dashboard Cite

Objective:To analyze the effects of nanoparticle-based dressings on the wound healing process in in vitro animals and human cells based on scientific evidence. Method: A systematic review of the literature in LILACS, PubMed and Science Direct databases. The articles were selected and evaluated for the level of evidence by the application of STROBE. Results: The sample consisted of 12 articles. The application of the products occurred in surgical wounds, burns, infected wounds and gingival ulcers in laboratory animals, as well as in vitro tests, demonstrating that among other advantages, the nanoparticle-based dressings increased the healing speed, had good antibacterial capacity and were non cytotoxic agents. Conclusion: Based on the analyzed articles, it can be affirmed that dressings containing nanocomposites are quite promising and are shown as a great therapeutic option in wound healing.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Fan et al 2013 (25) GNPs-CS/KGM as hemostatic first aid wound dressing with antibiotic effect: In vitro and in vivo study…”

Section: A10mentioning

confidence: 99%

Utilização de nanopartículas no tratamento de feridas: revisão sistemática

Silva

Aguiar

Rodrigues

et al. 2018

Rev. esc. enferm. USP

View full text Add to dashboard Cite

show abstract

“…The same approach to in vitro testing is likely to have similar significant human health implications (and very possibly commercially-expensive consequences) in the field of nano-medicine and nanosafety testing. In short, the same caveats of physiological relevance and predictive value are likely to apply in the field of nanotoxicology and nano-efficacy testing, with reports of consistency between in vivo and in vitro results from immortalised cell lines (48)(49)(50)(51)(52)(53)(54)(55)(56)(57) being fundamentally compromised by crossspecies extrapolation unjustified by model biochemical divergence.…”

Section: In Vitro Systems: Primary Cells Vs Immortalised Cellsmentioning

confidence: 99%

Advanced in vitro systems for efficacy and toxicity testing in nanomedicine

Fabbrizi

Duff

Oliver

et al. 2014

European Journal of Nanomedicine

View full text Add to dashboard Cite

Abstract:The use of nanoparticles (NPs) in nanomedicine is becoming an established therapeutic strategy for transport and delivery of bioactive molecules across biological barriers to elicit a specific cytological response in a target cell population. The utility of NP-derivatised nanomedicines and their potential therapeutic benefits, together with their biosafety, are being explored in cell models that, in order to accurately predict clinical potential, must reflect the architecture and function of the tissues from which the cells originate. These cell-based models, and their application in preclinical nanomedicine, are the subject of this review. Models are considered expressly from a commercial perspective, for their use as screening tools to identify and optimise therapeutic constructs, rather than as research tools requiring minimal throughput. Commercial utility is discussed in terms of the sourcing and assembly of cell-based model components, likelihood of data outputs highly predictive of clinical performance, and the compromise between need for advanced 3D culture architectures and the incremental difficulty in assembling those structures cost-effectively for mediumthroughput analysis. The relative merits of cell lines and primary human cells are discussed, the latter with respect to their potential physiological relevance when cultured under permissive conditions conferred by a biologicallyrich micro-environment. Tools for assembly of such microenvironments are reviewed, and the practitioner's choices of commercial products enabling 3D cell culture are also critically evaluated, from the merits of cell aggregates and micro-tissues to the architectural attractions of recreating epithelial monolayers and multi-cell type reconstituted tissues. Additionally, pitfalls and practical challenges in co-assembly of cells and scaffolds/matrices are considered, with the intention of signposting routes to consistent, scalable production of 3D cell cultures capable of screening the nanomedical potential of NP-conjugated therapeutics.

show abstract

“…Histological observations showed that these films improved considerably granulation tissue and epithelial coverage (developed by seven and fourteen days of treatment). Fan et al (98) discussed the use of chitosan/konjac glucomannan (CS/KGM) films embedded with antibiotic as a haemostatic wound dressing as another wound care option. Vetvicka and Vetvickova (99) reported that yeast-derived insoluble β (1-3)-D-glucans inhibited strongly adipogenesis, accelerated wound healing and lowered skin irritation.…”

Section: Woundsmentioning

confidence: 99%