GMWI-webtool: a user-friendly browser application for assessing health through metagenomic gut microbiome profiling

Abstract

Summary We recently introduced the Gut Microbiome Wellness Index, a stool metagenome-based indicator for assessing health by determining the likelihood of disease given the state of one’s gut microbiome. The calculation of our wellness index depends on the relative abundances of health-prevalent and health-scarce species. Encouragingly, GMWI has already been utilized in various studies focusing on differences in the gut microbiome between cases and controls. Herein, we introduce the GMWI-webtool, a user-friendly browser application that computes GMWI, health-prevalent/-scarce species’ relative abundances, and α-diversities from stool shotgun metagenome taxonomic profiles. Users of our interactive online tool can visualize their results and compare them side-by-side with those from our pooled reference dataset of metagenomes, as well as export data in.csv format and high-resolution figures. Availability and implementation GMWI-webtool is freely available here: https://gmwi-webtool.github.io/. Source code: https://github.com/danielchang2002/GMWI-webtool. Supplementary information Supplementary data are available at Bioinformatics online.

Evaluating the prebiotic effect of oligosaccharides on gut microbiome wellness using in vitro fecal fermentation

“…The Gut Microbiome Wellness Index (GMWI) was calculated using GMWI-webtool, a user-friendly browser application that computes GMWI from shotgun metagenome taxonomic profiles of stool 50 . Briefly, 50 gut microbial species associated with healthy (i.e., absent of disease) and non-healthy (i.e., having a clinical diagnosis of at least one disease) people were used to formulate GMWI.…”

Gut Microbiome Wellness Index 2 for Enhanced Health Status Prediction from Gut Microbiome Taxonomic Profiles

“…The potential of the gut microbiome as a marker for deciphering complex, chronic diseases has captivated the scientific community—in response, we recently developed the Gut Microbiome Wellness Index (GMWI) [previously called the Gut Microbiome Health Index (GMHI)] 10 . GMWI is a first-of-its-kind stool metagenome-based indicator for assessing health by determining the likelihood of an individual harboring a clinically diagnosed disease solely from their gut microbiome state, irrespective of the specific disease type 10,11 . This disease-agnostic index was derived from a comprehensive analysis of a pooled dataset comprising 4347 stool shotgun metagenomes from 34 independent studies.…”

“…Looking ahead, GMWI2 represents a timely pivotal tool for evaluating health based on an individual's unique gut microbial composition, paving the way for the early screening of adverse gut health shifts. GMWI2 is offered as an open-source command-line tool, ensuring it is both accessible to and adaptable for researchers interested in the translational applications of human gut microbiome science.indicator for assessing health by determining the likelihood of an individual harboring a clinically diagnosed disease solely from their gut microbiome state, irrespective of the specific disease type 10,11 . This disease-agnostic index was derived from a comprehensive analysis of a pooled dataset comprising 4347 stool shotgun metagenomes from 34 independent studies.…”

“…indicator for assessing health by determining the likelihood of an individual harboring a clinically diagnosed disease solely from their gut microbiome state, irrespective of the specific disease type 10,11 . This disease-agnostic index was derived from a comprehensive analysis of a pooled dataset comprising 4347 stool shotgun metagenomes from 34 independent studies.…”