GmDof41 regulated by the DREB1-type protein improves drought and salt tolerance by regulating the DREB2-type protein in soybean

Wei, Ji-Tong; Zhao, Shu-Ping; Zhang, Huiyuan; Jin, Long-Guo; Yu, Tong; Zheng, Lei; Ma, Jing; Zhou, Yongbin; Chen, Ming; Fu, Jin-Dong; Ma, You-Zhi; Xu, Zhao-Shi

doi:10.1016/j.ijbiomac.2023.123255

Cited by 8 publications

(4 citation statements)

References 73 publications

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“…Arabidopsis that overexpressing MtDof32, which was divided into group D1, had stronger salt tolerance than the wild-type plants [61]. In addition, soybean GmDof41 positively regulated salt tolerance by binding with the promoter of GmDREB2A [62]. It is worth noting that MpDof25 was induced by drought, salt, and low temperature, but its expression increased by about 140 times in 3 h of drought treatment, which suggested that MpDof25 may be a drought-sensitive gene.…”

Section: Discussionmentioning

confidence: 97%

Genome-Wide Identification and Expression Analysis of the Dof Transcription Factor in Annual Alfalfa Medicago polymorpha

Yang

Min

Wei

et al. 2023

Plants

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The Dof transcription factor is a plant-specific transcription gene family that plays various biological functions in plant development and stress response. However, no relevant research has been conducted on Medicago polymorpha. Here, 36 MpDof genes were identified in the M. polymorpha genome and further divided into 10 groups based on the comparative phylogenetic analysis. The essential information of MpDof genes, such as chromosomal localization, gene structure, conserved motifs, and selective pressures were systematically analyzed. All 36 MpDof genes were predicted to contain more cis-acting elements related to hormone response. MpDof24 and MpDof25 were predicted to interact with MpDof11 and MpDof26 to involve in the photoperiod blooms process. The MpDof genes showed a diverse expression pattern in different tissues. Notably, MpDof29 and MpDof31 were specifically expressed in the large pod and root, respectively, suggesting their crucial role in the pod and root development. qRT-PCR analysis indicated that the expression levels of MpDof10, MpDof25, MpDof26, and MpDof29 were obviously up-regulated under drought, salt, and cold stress. Collectively, genome-wide identification, evolutionary, and expression analysis of the Dof transcription gene family in M. polymorpha will provide new information to further understand and utilize the function of these Dof genes in Medicago plants.

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Section: Discussionmentioning

confidence: 97%

Genome-Wide Identification and Expression Analysis of the Dof Transcription Factor in Annual Alfalfa Medicago polymorpha

Yang

Min

Wei

et al. 2023

Plants

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“…Moreover, ABA upregulates SsDof7 and SsDof10, and SsDof18 was downregulated (Cai et al 2020). A recent study on soybeans showed that GmDof41 is involved in drought, salt, and exogenous ABA stress responses, and GmDof41 overexpression could improve plant sensitivity (Wei et al 2023). In our study, under drought and ABA treatments, the expression levels of ZmDof06, ZmDof19, ZmDof22, ZmDof38, and ZmDof46 significantly increased; however, those of ZmDof01, ZmDof08, ZmDof11, ZmDof33 and ZmDof39 significantly decreased.…”

Section: Discussionmentioning

confidence: 99%

Transcription factor ZmDof22 enhances drought tolerance by regulating stomatal movement and antioxidant enzyme activities in maize (Zea mays L.)

Cao,

Feiyu,

Fahim

et al. 2024

Preprint

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Identifying drought stress regulatory genes is essential for the genetic improvement of maize yield. Deoxyribonucleic acid binding with one finger (Dof), a plant-specific transcription factor family, is involved in signal transduction, morphogenesis, and environmental stress responses. In the present study, by weighted correlation network analysis (WGCNA) and gene coexpression network analysis, 15 putative Dof genes that respond to drought and rewatering were identified from maize. A real-time fluorescence quantitative PCR showed that these 15 genes were strongly induced by drought and ABA treatment, and among them, ZmDof22 was highly induced by drought and ABA treatment. Its expression level increased nearly 200 times after drought stress and more than 50 times after ABA treatment. After the normal conditions were restored, the expression levels were almost 100 times and 40 times those before treatment, respectively. The Gal4-LexA/UAS system and transcriptional activation analysis indicate that ZmDof22 is a transcriptional activator regulating maize drought tolerance and recovery ability. Further, overexpressed transgenic and mutant plants of ZmDof22 by CRISPR/Cas9 show that the ZmDof22 improves maize drought tolerance by promoting stomatal closure, reduces water loss, and enhances antioxidant enzyme activity by participating in the ABA pathways. Taken together, our findings laid a foundation for further functional studies of the ZmDof gene family and provided insights into the role of the ZmDof22 regulatory network in controlling drought tolerance and recovery ability of maize.

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“…During their long evolution, plants have developed many responses to salt stress, such as calcium signals and glutathione-anti-circulating pathways to maintain cell homeostasis and eliminate reactive oxygen species [ 7 , 60 ]. The mechanisms of salt tolerance-related genes have been identified in some species, such as AtHKT1;1, AtHAK5, OsLAX, OsABCB, SOS2, SnRK2, GmDof41, SlWRKY23, etc., and these genes have endowed plants with different degrees of tolerance to salt stress [ 61 , 62 , 63 , 64 , 65 , 66 , 67 ]. The ascorbate–glutathione (ASA-GSH) cycle is widespread in living organisms and plays an essential role in plant oxidative defenses and abiotic stress responses [ 31 , 68 ].…”

Section: Discussionmentioning

confidence: 99%

GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.)

Pang

Zhong

et al. 2023

IJMS

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Salt stress has a detrimental impact on crop yield, quality, and profitability. The tau-like glutathione transferases (GSTs) represent a significant group of enzymes that play a crucial role in plant stress responses, including salt stress. In this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. Expression pattern analysis revealed that GmGSTU23 was predominantly expressed in the roots and flowers and exhibited a concentration–time-specific pattern in response to salt stress. Transgenic lines were generated and subjected to phenotypic characterization under salt stress. The transgenic lines exhibited increased salt tolerance, root length, and fresh weight compared to the wild type. Antioxidant enzyme activity and malondialdehyde content were subsequently measured, and the data revealed no significant differences between the transgenic and wild-type plants in the absence of salt stress. However, under salt stress, the wild-type plants exhibited significantly lower activities of SOD, POD, and CAT than the three transgenic lines, whereas the activity of APX and the content of MDA showed the opposite trend. We identified changes in glutathione pools and associated enzyme activity to gain insights into the underlying mechanisms of the observed phenotypic differences. Notably, under salt stress, the transgenic Arabidopsis’s GST activity, GR activity, and GSH content were significantly higher than those of the wild type. In summary, our findings suggest that GmGSTU23 mediates the scavenging of reactive oxygen species and glutathione by enhancing the activity of glutathione transferase, thereby conferring enhanced tolerance to salt stress in plants.

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GmDof41 regulated by the DREB1-type protein improves drought and salt tolerance by regulating the DREB2-type protein in soybean

Cited by 8 publications

References 73 publications

Genome-Wide Identification and Expression Analysis of the Dof Transcription Factor in Annual Alfalfa Medicago polymorpha

Genome-Wide Identification and Expression Analysis of the Dof Transcription Factor in Annual Alfalfa Medicago polymorpha

Transcription factor ZmDof22 enhances drought tolerance by regulating stomatal movement and antioxidant enzyme activities in maize (Zea mays L.)

GmGSTU23 Encoding a Tau Class Glutathione S-Transferase Protein Enhances the Salt Tolerance of Soybean (Glycine max L.)

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