GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure

Huang, Fei; Dong, Xifeng; Zhang, Luping; Zhang, Xuejun; Bai, Xianyong; Li, Zhenzhong

doi:10.1016/j.jocn.2009.07.112

Cited by 11 publications

(10 citation statements)

References 24 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NGF upregulation by CCS-HE showed a transient pattern and NF160 did show a prolonged upregulation by CCS-HE ( Figure 2). This is consistent with literature reports that NGF can regulate NF160 expression (Huang et al, 2010;Schimmelpfeng et al, 2004) and NF160 is one of three neurofilaments for the core structures of the neurite outgrowth and axonal elongation (Angelucci et al, 2014;Barnes & Polleux, 2009;Cheng & Poo, 2012). This is also consistent with morphologic change of human SH-SY5Y cells induced by CCS-HE even though we did not measure both NGF and NF160 expression at an earlier stage (e.g., 12 hr), suggesting that CCS-HE stimulated neurite outgrowth by upregulation of NGF expression and NGF further turned on NF160 expression for neurite outgrowth.…”

Section: Discussionsupporting

confidence: 94%

“…Studies have shown that NGF causes axonal branching and elongation (Madduri, Papaloizos, & Gander, 2009) and NGF could induce the expression of NF proteins (Huang et al, 2010;Schimmelpfeng, Weibezahn, & Dertinger, 2004). In contrast, NGF defect results in an undergoing apoptosis in neurons (Freeman et al, 2004).…”

Section: Ccsmentioning

confidence: 99%

“…Nerve growth factor (NGF) is a classic growth factor in family of neurotrophic factors, a group of proteins that are mainly synthesized and secreted by neurons and astrocytes and that are crucial for neuronal survival, growth, and differentiation (Sofroniew, Howe, & Mobley, 2001). Studies have shown that NGF causes axonal branching and elongation (Madduri, Papaloizos, & Gander, 2009) and NGF could induce the expression of NF proteins (Huang et al., 2010; Schimmelpfeng, Weibezahn, & Dertinger, 2004). In contrast, NGF defect results in an undergoing apoptosis in neurons (Freeman et al., 2004).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An appropriate ratio of unsaturated fatty acids is the constituent of hickory nut extract for neurite outgrowth in human SH‐SY5Y cells

Gao

Zhou

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

Carya cathayensis Sarg (CCS) has been used as a conventional folk medical nut for health care in China for a long time. In the "Bencao Gangmu," an ancient Chinese book on herbal medicine, it was described that the pecan nuts of CCS were beneficial for brain and kidney health. Traditionally, one of the main benefits of CCS was considered to help our brain by improving the memory ability and delaying the neuronal degeneration. Current nutrition research has indicated that CCS contains a variety of nutrients, including high levels of unsaturated fatty acids (UFAs) that are supported by the harmonious expression of key ones for UFAs (Huang et al., 2016). It

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Ccsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An appropriate ratio of unsaturated fatty acids is the constituent of hickory nut extract for neurite outgrowth in human SH‐SY5Y cells

Gao

Zhou

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…In contrast, the levels of GM1a, a major ganglioside in the central nervous system, increased in spinal cord of SOD1(G86R) mice at the symptomatic stage. Interestingly, GM1 has been shown to exhibit neurotrophic properties in vitro ( 48 , 49 ) and in vivo ( 50 ). It is therefore tempting to suggest that the increase in GM1a we found in our mice might serve at least in part to counteract the pathological process.…”

Section: Discussionmentioning

confidence: 99%

Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase

et al. 2015

View full text Add to dashboard Cite

Amyotrophic lateral sclerosis (ALS) is a fatal adult-onset disease characterized by upper and lower motor neuron degeneration, muscle wasting and paralysis. Growing evidence suggests a link between changes in lipid metabolism and ALS. Here, we used UPLC/TOF-MS to survey the lipidome in SOD1(G86R) mice, a model of ALS. Significant changes in lipid expression were evident in spinal cord and skeletal muscle before overt neuropathology. In silico analysis also revealed appreciable changes in sphingolipids including ceramides and glucosylceramides (GlcCer). HPLC analysis showed increased amounts of GlcCer and downstream glycosphingolipids (GSLs) in SOD1(G86R) muscle compared with wild-type littermates. Glucosylceramide synthase (GCS), the enzyme responsible for GlcCer biosynthesis, was up-regulated in muscle of SOD1(G86R) mice and ALS patients, and in muscle of wild-type mice after surgically induced denervation. Conversely, inhibition of GCS in wild-type mice, following transient peripheral nerve injury, reversed the overexpression of genes in muscle involved in oxidative metabolism and delayed motor recovery. GCS inhibition in SOD1(G86R) mice also affected the expression of metabolic genes and induced a loss of muscle strength and morphological deterioration of the motor endplates. These findings suggest that GSLs may play a critical role in ALS muscle pathology and could lead to the identification of new therapeutic targets.

show abstract

“…4. GM1 can affect the morphological changes of DRG neurons and protect the DRG neurons from damage induced by excitotoxic glutamate [21]. Considering DRG neurons are a target for neurotoxicity induced by oxaliplatin, GM1 may exert its neuroprotective effects by affecting DRG neurons.…”

Section: Introductionmentioning

confidence: 99%

Ganglioside-monosialic acid (GM1) prevents oxaliplatin-induced peripheral neurotoxicity in patients with gastrointestinal tumors

et al. 2013

View full text Add to dashboard Cite

BackgroundOxaliplatin, an effective antineoplastic agent againstgastrointestinal tumors, can cause severe peripheral neurotoxicity, which seriously limits its clinical application. To date, there are no effective treatments for this complication. Ganglioside-monosialic acid (GM1) has been shown to protect neurons against injuries and degeneration. The aim of this study was to evaluate the effects of GM1 on preventing oxaliplatin-induced neurotoxicity in patients with gastrointestinal tumors.MethodsIn this study, 120 patients with gastrointestinal tumors were enrolled, andthey received the treatment of XELOX (oxaliplatin and capecitabine) and FOLFOX4 (oxaliplatin, leukovolin and 5-fluorouracil). The patients were randomly divided into two groups, the experimental group and control group, with60 patients ineach. On the day chemotherapy was initiated, the experimental group received GM1 intravenously (100 mg once daily) for 3 days, while no neuroprotective agents were applied in the control group. The incidence rates and classification of neurotoxicity in the two groups were evaluated and the differences between the two groups were examined. Furthermore, whether GM1 affected the therapeutic effects of chemotherapy was also examined.ResultsThe grade of neurotoxicity in the experimental group was significantly lower than in the control group (P<0.05, Mann–Whitney U test). The probability of occurrence of low-grade neurotoxicity (grade 0 and 1) in the experimental group was higher than that in the control group (logistic ordinal regression); whereas the probability of occurrence of high-grade neurotoxicity (grade 2 and 3) in the experimental group was lower than in the control group (logistic ordinal regression).ConclusionThe data suggested that GM1 could reduce the grade of oxaliplatin-induced neurotoxicity and was an effective neuroprotective agent against oxaliplatin-induced high-grade neurotoxicity in patients with gastrointestinal tumors.

show abstract

GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure

Cited by 11 publications

References 24 publications

An appropriate ratio of unsaturated fatty acids is the constituent of hickory nut extract for neurite outgrowth in human SH‐SY5Y cells

An appropriate ratio of unsaturated fatty acids is the constituent of hickory nut extract for neurite outgrowth in human SH‐SY5Y cells

Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase

Ganglioside-monosialic acid (GM1) prevents oxaliplatin-induced peripheral neurotoxicity in patients with gastrointestinal tumors

Contact Info

Product

Resources

About