GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner

Korniotis, Sarantis; Saichi, Melissa; Trichot, Coline; Hoffmann, Caroline; Amblard, Elise; Viguier, Annick; Grondin, Sophie; Noël, Floriane; Mattoo, Hamid; Soumelis, Vassili

doi:10.1242/jcs.260298

Cited by 4 publications

(1 citation statement)

References 72 publications

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“…The link between GM-CSF and HIF-1α was established, demonstrating the bactericidal activity of CSF2 in this “pathway” and assisting CD4 + T cells in suppressing Mtb infection ( Van Dis et al., 2022 ). CSF2 promotes Tfh1 cell polarization by activating dendritic cells (DCs) in a CD40-dependent manner ( Korniotis et al., 2022 ), and also up-regulates anti-apoptosis-related genes such as Bcl-2 and HSP27 to maintain the survival of macrophages that have been infected with Mtb. The ability of macrophages to secrete CSF2 is significantly negatively correlated with intracellular bacterial load.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Wei,

Guo,

Song

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundMycobacterium tuberculosis antigen (Mtb-Ag) is a polypeptide component with a molecular weight of 10-14 kDa that is obtained from the supernatant of the H37Ra strain after heat treatment. It stimulates the activation and proliferation of γδT cells in the blood to produce an immune response against tuberculosis. Mtb-Ag is therefore crucial for classifying and detecting the central genes and key pathways involved in TB initiation and progression.MethodsIn this study, we performed high-throughput RNA sequencing of peripheral blood mononuclear cells (PBMC) from Mtb-Ag-stimulated and control samples to identify differentially expressed genes and used them for gene ontology (GO) and a Kyoto Encyclopedia of Genomes (KEGG) enrichment analysis. Meanwhile, we used PPI protein interaction network and Cytoscape analysis to identify key genes and qRT-PCR to verify differential gene expression. Single-gene enrichment analysis (GSEA) was used further to elucidate the potential biological functions of key genes. Analysis of immune cell infiltration and correlation of key genes with immune cells after Mtb-Ag-stimulated using R language.ResultsWe identified 597 differentially expressed genes in Mtb-Ag stimulated PBMCs. KEGG and GSEA enrichment analyzed the cellular pathways related to immune function, and DEGs were found to be primarily involved in the TNF signaling pathway, the IL-17 signaling pathway, the JAK-STAT signaling pathway, cytokine-cytokine receptor interactions, and the NF-κB signaling pathway. Wayne analysis using GSEA, KEGG, and the protein-protein interaction (PPI) network showed that 34 genes, including PTGS2, IL-1β, IL-6, TNF and IFN-γ et al., were co-expressed in the five pathways and all were up-regulated by Mtb-Ag stimulation. Twenty-four DEGs were identified using qRT-PCR, including fourteen up-regulated genes (SERPINB7, IL20, IFNG, CSF2, PTGS2, TNF-α, IL36G, IL6, IL10, IL1A, CXCL1, CXCL8, IL4, and CXCL3) and ten down-regulated genes (RTN1, CSF1R CD14, C5AR1, CXCL16, PLXNB2, OLIG1, EEPD1, ENG, and CCR1). These findings were consistent with the RNA-Seq results.ConclusionThe transcriptomic features associated with Mtb-Ag provide the scientific basis for exploring the intracellular immune mechanisms against Mtb. However, more studies on these DEGs in pathways associated with Mtb-Ag stimulation are needed to elucidate the underlying pathologic mechanisms of Mtb-Ag during Mtb infection.

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Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Wei,

Guo,

Song

et al. 2023

Front. Cell. Infect. Microbiol.

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Single‐cell sequencing: Current applications in various tuberculosis specimen types

Zeng,

Ma,

Chen

et al. 2024

Cell Proliferation

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Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (M.tb) and responsible for millions of deaths worldwide each year. It has a complex pathogenesis that primarily affects the lungs but can also impact systemic organs. In recent years, single‐cell sequencing technology has been utilized to characterize the composition and proportion of immune cell subpopulations associated with the pathogenesis of TB disease since it has a high resolution that surpasses conventional techniques. This paper reviews the current use of single‐cell sequencing technologies in TB research and their application in analysing specimens from various sources of TB, primarily peripheral blood and lung specimens. The focus is on how these technologies can reveal dynamic changes in immune cell subpopulations, genes and proteins during disease progression after M.tb infection. Based on the current findings, single‐cell sequencing has significant potential clinical value in the field of TB research. Next, we will focus on the real‐world applications of the potential targets identified through single‐cell sequencing for diagnostics, therapeutics and the development of effective vaccines.

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First person – Sarantis Korniotis

2022

Journal of Cell Science

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First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping researchers promote themselves alongside their papers. Sarantis Korniotis is first author on ‘ GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner’, published in JCS. Sarantis conducted the research described in this article while a postdoctoral researcher in Professor Vassili Soumelis's lab at Saint-Louis Hospital, Université de Paris, France. He is now a Senior Scientific Associate in Immunology at Intelligencia AI, Athens, Greece, investigating new signals and factors that make cells acquire regulatory or immune-suppressive properties.

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GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner

Cited by 4 publications

References 72 publications

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen

Single‐cell sequencing: Current applications in various tuberculosis specimen types

First person – Sarantis Korniotis

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