Glyphosate resistance reduces kochia fitness: Comparison of segregating resistant and susceptible F2 populations

Martin, Sara L.; Benedict, Leshawn; Sauder, Connie A.; Wei, Wei; Costa, Leandro Oliveira; Hall, Linda M.; Beckie, Hugh J.

doi:10.1016/j.plantsci.2017.04.010

Cited by 37 publications

(46 citation statements)

References 37 publications

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“…Inbred K. scoparia individuals with two vs 14 copies of EPSPS showed no differences in fitness traits (Kumar & Jha, ). Another study assessed six segregating F 2 K. scoparia populations in which fitness was compared between individuals with low (one) vs high (10) EPSPS copy number under intraspecific competition within each population (Martin et al ., ). Overall, the effect of EPSPS gene amplification on plant fitness traits depended on the particular population genetic background.…”

Section: Does Glyphosate Resistance By Epsps Gene Amplification and Omentioning

confidence: 97%

“…Plants from four populations, each with 10 EPSPS copies, showed no decreased fitness as compared with plants with no EPSPS gene amplification. In two other K. scoparia populations with 10 copies, however, plants showed average reductions of 70% and 75% in individual seed weight production and viability, respectively (Martin et al ., ). No fitness cost was identified in various K. scoparia populations from Kansas (USA) in which glyphosate‐resistant individuals exhibited an average of five to six EPSPS gene copy numbers (Osipitan & Dille, ).…”

Section: Does Glyphosate Resistance By Epsps Gene Amplification and Omentioning

confidence: 97%

“…Based on the evolution of gene expression and resource allocation theory (reviewed in Herms & Mattson, ; Bergelson & Purrington, ; Lynch & Marinov, ), EPSPS gene amplification or overexpression should attract plant fitness penalties due to a metabolic cost. Some empirical evidence has validated this hypothesis, showing that evolved overproduction of EPSPS and downstream products incurs a fitness cost (Cockerton, ; Yanniccari et al ., ; Martin et al ., ; Wu et al ., ). However, the basis of this constrained energy budget under EPSPS amplification has been challenged, not only by those cases in which a cost has not been detectable (Giacomini et al ., ; Vila‐Aiub et al ., ; Kumar & Jha, ; Martin et al ., ; Osipitan & Dille, ), but also in those studies reporting on a fitness advantage endowed by EPSPS overexpression in transgenic plants (Lu et al ., ,b; Wang et al ., ; Yang et al ., ; Beres et al ., ; Fang et al ., ).…”

Section: Final Remarksmentioning

confidence: 99%

“…Although it is reasonable to expect that the process of natural selection could have minimized the costs associated with EPSPS gene amplification (Andersson, ; Paris et al ., ; Darmency et al ., ), studies conducted over a single plant generation may not detect subtle fitness differences (Giacomini et al ., ; Vila‐Aiub et al ., ; Kumar & Jha, ; Martin et al ., ) which only manifest themselves after several generations of additive fitness cost effects (each of which could also slowly reduce the frequency of plants carrying the amplified gene) (Vila‐Aiub et al ., , , ). For instance, the frequency of A. tuberculatus plants with up to five EPSPS gene copies grown in competition decreased from 50% to less than 5% after six generations without glyphosate treatment (Wu et al ., ).…”

Section: Does Glyphosate Resistance By Epsps Gene Amplification and Omentioning

confidence: 99%

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Do plants pay a fitness cost to be resistant to glyphosate?

Vila‐Aiub

Powles

2019

New Phytologist

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Summary We reviewed the literature to understand the effects of glyphosate resistance on plant fitness at the molecular, biochemical and physiological levels. A number of correlations between enzyme characteristics and glyphosate resistance imply the existence of a plant fitness cost associated with resistance‐conferring mutations in the glyphosate target enzyme, 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS). These biochemical changes result in a tradeoff between the glyphosate resistance of the EPSPS enzyme and its catalytic activity. Mutations that endow the highest resistance are more likely to decrease catalytic activity by reducing the affinity of EPSPS for its natural substrate, and/or slowing the velocity of the enzyme reaction, and are thus very likely to endow a substantial plant fitness cost. Prediction of fitness costs associated with EPSPS gene amplification and overexpression can be more problematic. The validity of cost prediction based on the theory of evolution of gene expression and resource allocation has been cast into doubt by contradictory experimental evidence. Further research providing insights into the role of the EPSPS cassette in weed adaptation, and estimations of the energy budget involved in EPSPS amplification and overexpression are required to understand and predict the biochemical and physiological bases of the fitness cost of glyphosate resistance.

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Section: Does Glyphosate Resistance By Epsps Gene Amplification and Omentioning

confidence: 97%

Section: Does Glyphosate Resistance By Epsps Gene Amplification and Omentioning

confidence: 97%

Section: Final Remarksmentioning

confidence: 99%

Section: Does Glyphosate Resistance By Epsps Gene Amplification and Omentioning

confidence: 99%

See 2 more Smart Citations

Do plants pay a fitness cost to be resistant to glyphosate?

Vila‐Aiub

Powles

2019

New Phytologist

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“…On the other hand, Martin et al . () found delayed phenology (emergence, flowering) of GR compared with GS full siblings from segregating F2 populations generated from within six K. scoparia populations from western Canada; overall, they concluded that glyphosate resistance reduces K. scoparia fitness. An important question is whether or not these differences in germination and phenology between GR and GS K. scoparia populations can be successfully or practically exploited through delayed preseeding weed control, so that efficacy is equal on both genotypes.…”

Section: Discussionmentioning

confidence: 97%

Seedbank persistence, germination and early growth of glyphosate‐resistant Kochia scoparia

Beckie

Blackshaw²,

Leeson

et al. 2018

Weed Research

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Summary This study describes the seedbank persistence of glyphosate‐resistant (GR) Kochia scoparia at two sites in western Canada and examines if GRK. scoparia from western Canada and mid‐western United States (USA) differ from their susceptible counterparts in seed germination and early growth characteristics at low‐temperature regimes. Site or depth of seed burial (surface, 2.5 cm, 10 cm) did not affect seed viability over time and time to 50% and 90% loss of viability averaged 210 and 232 days respectively. Glyphosate‐resistant K. scoparia generally germinated later and had lower cumulative germination than glyphosate‐susceptible (GS) K. scoparia from Saskatchewan, Canada; and Kansas, USA; but not Colorado, USA. Similarly, time to 10% first leaf of GSK. scoparia from Saskatchewan and Kansas tended to be sooner than that of GRK. scoparia, with a greater percentage of GS vs. GR seedlings of populations from all regions having attained first leaf by the end of the experiment. The short seedbank longevity and delayed and reduced germination and time to first leaf of GRK. scoparia may potentially be exploited to maximise management efficacy through delayed preseeding weed control or alternatively by early seeding date to enhance crop competitiveness.

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