Glyphosate-Degrading Microorganisms from Industrial Activated Sludge

Balthazor, T. M.; Hallas, Laurence E.

doi:10.1128/aem.51.2.432-434.1986

Cited by 144 publications

(52 citation statements)

References 8 publications

(5 reference statements)

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“…First, glyphosate might have been degraded by micro‐organisms to sarcosine, inorganic phosphate (Dick and Quinn ) or aminomethylphosphonic acid (AMPA) (Rueppel et al . ; Balthazor and Hallas ). Determination of these substances was not included in this study.…”

Section: Discussionmentioning

confidence: 96%

Investigations on the possible impact of a glyphosate-containing herbicide on ruminal metabolism and bacteriain vitroby means of the ‘Rumen Simulation Technique’

et al. 2016

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Aims: This study was performed in a well-established in vitro model to investigate whether the application of a glyphosate-containing herbicide might affect the bacterial communities and some biochemical parameters in a cow's rumen. Methods and Results: The test item was applied in two concentrations (high and low) for 5 days. In a second trial, fermentation vessels were inoculated with Clostridium sporogenes before the high dose was applied. Effluents were analysed by biochemical, microbiological and genetic methods. A marginal increase in short-chain fatty acid production and a reduction in NH 3 -N were observed. There were minor and rather equivocal changes in the composition of ruminal bacteria but no indications of a shift towards a more frequent abundance of pathogenic Clostridia species. Clostridium sporogenes counts declined consistently. Conclusions: No adverse effects of the herbicide on ruminal metabolism or composition of the bacterial communities could be detected. In particular, there was no evidence of a suspected stimulation of Clostridia growth. Significance and Impact of the Study: Antibiotic activity of glyphosate resulting in microbial imbalances has been postulated. In this exploratory study, however, intraruminal application of concentrations reflecting potential exposure of dairy cows or beef cattle did not exhibit significant effects on bacterial communities in a complex in vitro system. The low number of replicates (n = 3/dose) may leave some uncertainty.

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Section: Discussionmentioning

confidence: 96%

Investigations on the possible impact of a glyphosate-containing herbicide on ruminal metabolism and bacteriain vitroby means of the ‘Rumen Simulation Technique’

et al. 2016

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“…Glyphosate is rapidly degraded in the environment, and a number of bacterial species have been isolated which can break down the compound. These include a Flavobacterium species [21], several Pseudomonas species [22][23][24][25] (of which the best studied is Pseudomonas sp. PG2982 [23]), an Alcaligenes isolate [24], Bacillus megaterium strain 2BLW [22], Arthrobacter sp.…”

Section: Phosphonate Xenobiotics -Glyphosatementioning

confidence: 99%

Microbial metabolism of sulfurand phosphorus-containing xenobiotics

Kertesz

Cook

Leisinger

1994

FEMS Microbiology Reviews

121

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Abstract:The enzymes involved in the microbial metabolism of many important phosphorus-or sulfur-containing xenobiotics, including organophosphate insecticides and precursors to organosulfate and organosulfonate detergents and dyestuffs have been characterized. In several instances their genes have been cloned and analysed. For phosphonate xenobiotics, the enzyme system responsible for the cleavage of the carbon-phosphorus bond has not yet been observed in vilro, though much is understood on a genetic level about phosphonate degradation. Phosphonate metabolism is regulated as part of the Pho regulon, under phosphate starvation control. For organophosphorothionate pesticides the situation is not so clear, and the mode of regulation appears to depend on whether the compounds are utilized to provide phosphorus, carbon or sulfur for cell growth. The same is true for organosulfonate metabolism, where different (and differently regulated) enzymatic pathways are involved in the utilization of sulfonates as carbon and as sulfur sources, respectively. Observations at the protein level in a number of bacteria suggest that a regulatory system is present which responds to sulfate limitation and controls the synthesis of proteins involved in providing sulfur to the cell and which may reveal analogies between the regulation of phosphorus and sulfur metabolism.

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“…la Cecilia and Maggi, Stochastic sensitivity analysis of Glyphosate biochemical degradation Table 1. Biochemical reactions implemented in the numerical solver together with their corresponding kinetic parameters as estimated in la Cecilia & Maggi (2017) against laboratory observations published in (a) Balthazor and Hallas (1986); Jacob et al (1988); (b) Moore et al (1983); (c) Mcauliffe et al (1990); (d) Balthazor and Hallas (1986); (e) Levering et al (1981); (f) Hippe et al (1979); (g) Appleyard and Woods (1956); (h) Hormann and Andreesen (1989); (i) Därre and Andreesen (1982); (l) B HyO was assumed to grow on CH 2 O as an independent reaction, with MMM kinetic parameters averaged from estimations against experiments in Balthazor and Hallas (1986); Jacob et al (1988); Moore et al (1983)…”

Section: P1r3bmentioning

confidence: 99%

“…The bacteria mortality rate δ (s -1 ) was assumed to be constant and equal to 10 -6 s -1 after Gastrin and Marcetic (1968). Phosphate (PO 4 3-) inhibitory effect on GLP and AMPA biodegradation along P1R1 and P1R2, respectively, was accounted for using an inhibition value K I = 2.53×10 -4 M estimated against observations in Balthazor and Hallas (1986). Substrate competition was not included in this work due to the limited variety of substrates available.…”

Section: Modellingmentioning

confidence: 99%

Stochastic sensitivity analysis of glyphosate biochemical degradation

2017

Syme, G., Hatton MacDonald, D., Fulton, B. And Piantadosi, J. (Eds) MODSIM2017, 22nd International Congress on Modelling and Si

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A comprehensive numerical modelling of soil biogeochemical dynamics allowed us to explore uncertainties in the fate of the herbicide glyphosate (GLP) and its crucial byproduct aminomethylphosphonic acid (AMPA) in soil. GLP and AMPA are both toxic and have the potential to disrupt complex ecological and biogeochemical processes. This study aims at identifying the more influential sources of uncertainty in GLP biochemical degradation. Microorganisms may evolve different strategies for scavenging nutrients and energy from anthropogenic molecules depending on the surrounding environmental conditions. GLP can be catabolized by soil bacteria along two pathways. One biotic pathway produces AMPA, which can be degraded biologically to non-toxic end products, while a second biotic pathway produces non-toxic byproducts. Recent studies have shown that GLP and AMPA can also undergo fast chemical degradation to non-toxic byproducts in the presence of birnessite mineral, in which Mn 3+ and Mn 4+ ions act as catalysts. Therefore, a comprehensive GLP degradation reaction network was tested numerically by means of the BRTSim solver to assess GLP and AMPA degradation potential within a network that integrates several biochemical processes. Chemical and biological processes were described by Michaelis-Menten (MM) kinetics and the Monod growth model, respectively. The biochemical reactions describing the reaction network and the corresponding kinetic parameters were retrieved from the literature. In this numerical study, GLP was applied at typical rates in a soil control volume representing the top soil of an agricultural plot. GLP and AMPA concentrations were modelled over time as a function of both biological and chemical processes. A suite of sensitivity analyses on input Michaelis-Menten-Monod (MMM) parameters were run to assess the effect of biological parametric uncertainties and to quantify the influence of specific biological processes or specific group of MMM kinetic parameters to the overall model output. Parameter values were randomly chosen from a Gaussian distribution with mean equal to the corresponding experimentally-retrieved parameter value and standard deviation equal to 5, 10, 15, 20, 25, and 30% of that value. We found that, in the lack of birnessite mineral, variability in the reaction rate constant increased GLP equilibrium concentration, while variability in the half-saturation concentration constant and the biomass yield decreased it. The action of birnessite mineral shrank output variability and decreased GLP concentrations by 5 times. Overall, the more GLP was biodegraded the more AMPA was produced, which accumulated due to its slow biodegradation.

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Glyphosate-Degrading Microorganisms from Industrial Activated Sludge

Cited by 144 publications

References 8 publications

Investigations on the possible impact of a glyphosate-containing herbicide on ruminal metabolism and bacteriain vitroby means of the ‘Rumen Simulation Technique’

Investigations on the possible impact of a glyphosate-containing herbicide on ruminal metabolism and bacteriain vitroby means of the ‘Rumen Simulation Technique’

Microbial metabolism of sulfurand phosphorus-containing xenobiotics

Stochastic sensitivity analysis of glyphosate biochemical degradation

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