Glyoxylate Metabolism Is a Key Feature of the Metabolic Degradation of 1,4-Dioxane by Pseudonocardia dioxanivorans Strain CB1190

Grostern, Ariel; Sales, Christopher M.; Zhuang, Wei-Qin; Erbilgin, Onur; Alvarez‐Cohen, Lisa

doi:10.1128/aem.00067-12

Cited by 80 publications

(66 citation statements)

References 54 publications

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“…Identification of candidate biomarkers was focused on monooxygenase and dehydrogenase genes based on earlier work describing the dioxane degradation pathway for monooxygenase-expressing bacteria (29,36) and expression of dehydrogenase genes in bacteria during utilization of monooxygenase-inducing substrates (37)(38)(39). To facilitate gene selection, an in silico analysis was performed to identify all annotated monooxygenase groups within CB1190 and its associated plasmids (35).…”

Section: Methodsmentioning

confidence: 99%

“…Candidate targets were selected from this comprehensive list based on their functional roles in the biological transformation of recalcitrant compounds. Dehydrogenase gene targets were also selected based on sequence similarity to alcohol and aldehyde dehydrogenase genes previously shown to be associated with BMMs (15,29) or involved in the oxidation of primary substrates in monooxygenase-expressing bacteria (38,39).…”

Section: Methodsmentioning

confidence: 99%

“…strain ENV478. Indeed, further study of THFMO in CB1190 has shown this BMM was upregulated during dioxane biodegradation (29). Consequently, this gene cluster within CB1190 is also referred to as the dioxane monooxygenase (DXMO).…”

mentioning

confidence: 99%

“…Previous research efforts have determined that the microbial transformation of dioxane involves monooxygenase enzymes for both metabolic and cometabolic degradation pathways (28,29). Metabolic degradation is less common, with only a few microorganisms being able to use dioxane as the sole source for carbon and energy (18,(30)(31)(32).…”

mentioning

confidence: 99%

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Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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Bacterial multicomponent monooxygenase gene targets in Pseudonocardia dioxanivorans CB1190 were evaluated for their use as biomarkers to identify the potential for 1,4-dioxane biodegradation in pure cultures and environmental samples. Our studies using laboratory pure cultures and industrial activated sludge samples suggest that the presence of genes associated with dioxane monooxygenase, propane monooxygenase, alcohol dehydrogenase, and aldehyde dehydrogenase are promising indicators of 1,4-dioxane biotransformation; however, gene abundance was insufficient to predict actual biodegradation. A time course gene expression analysis of dioxane and propane monooxygenases in Pseudonocardia dioxanivorans CB1190 and mixed communities in wastewater samples revealed important associations with the rates of 1,4-dioxane removal. In addition, transcripts of alcohol dehydrogenase and aldehyde dehydrogenase genes were upregulated during biodegradation, although only the aldehyde dehydrogenase was significantly correlated with 1,4-dioxane concentrations. Expression of the propane monooxygenase demonstrated a time-dependent relationship with 1,4-dioxane biodegradation in P. dioxanivorans CB1190, with increased expression occurring after over 50% of the 1,4-dioxane had been removed. While the fraction of P. dioxanivorans CB1190-like bacteria among the total bacterial population significantly increased with decrease in 1,4-dioxane concentrations in wastewater treatment samples undergoing active biodegradation, the abundance and expression of monooxygenase-based biomarkers were better predictors of 1,4-dioxane degradation than taxonomic 16S rRNA genes. This study illustrates that specific bacterial monooxygenase and dehydrogenase gene targets together can serve as effective biomarkers for 1,4-dioxane biodegradation in the environment.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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“…1B). Note that a high intracellular glyoxylate concentration (expected in the glcB mutant) is likely to be toxic because glyoxylate can be transformed into toxic intermediates, such as glyoxal or glycolaldehyde (26).…”

Section: Physiological and Phenotypic Alterations In The Absence Of Thementioning

confidence: 99%

Role of Glyoxylate Shunt in Oxidative Stress Response

Ahn

Jung

Jang

et al. 2016

Journal of Biological Chemistry

197

161

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The glyoxylate shunt (GS) is a two-step metabolic pathway (isocitrate lyase, aceA; and malate synthase, glcB) that serves as an alternative to the tricarboxylic acid cycle. The GS bypasses the carbon dioxide-producing steps of the tricarboxylic acid cycle and is essential for acetate and fatty acid metabolism in bacteria. GS can be up-regulated under conditions of oxidative stress, antibiotic stress, and host infection, which implies that it plays important but poorly explored roles in stress defense and pathogenesis. In many bacterial species, including Pseudomonas aeruginosa, aceA and glcB are not in an operon, unlike in Escherichia coli. In P. aeruginosa, we explored relationships between GS genes and growth, transcription profiles, and biofilm formation. Contrary to our expectations, deletion of aceA in P. aeruginosa improved cell growth under conditions of oxidative and antibiotic stress. Transcriptome data suggested that aceA mutants underwent a metabolic shift toward aerobic denitrification; this was supported by additional evidence, including up-regulation of denitrification-related genes, decreased oxygen consumption without lowering ATP yield, increased production of denitrification intermediates (NO and N 2 O), and increased cyanide resistance. The aceA mutants also produced a thicker exopolysaccharide layer; that is, a phenotype consistent with aerobic denitrification. A bioinformatic survey across known bacterial genomes showed that only microorganisms capable of aerobic metabolism possess the glyoxylate shunt. This trend is consistent with the hypothesis that the GS plays a previously unrecognized role in allowing bacteria to tolerate oxidative stress.

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Practical Perspectives of 1,4‐Dioxane Investigation and Remediation

Chiang

Anderson

Wilken

et al. 2016

Remediation Journal

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1,4‐Dioxane (dioxane) is a contaminant of emerging concern that is classified by the U.S. Environmental Protection Agency as a likely human carcinogen. Dioxane has been used as a minor or major ingredient in many applications, and is also generated as an unwanted by‐product of industrial processes associated with the manufacturing of polyethylene, nonionic surfactants, and many consumer products (cosmetics, laundry detergents, shampoos, etc.). Dioxane is also a known stabilizer of chlorinated solvents, particularly 1,1,1‐trichloroethane, and has been commonly found comingled with chlorinated solvent plumes. Dioxane plumes at chlorinated solvent sites can complicate site closure strategies, which to date have not typically focused on dioxane. Aggressive treatment technologies have greatly advanced and are clearly capable of achieving lower parts per billion cleanup criteria using ex situ advanced oxidation processes and sorption media. In situ chemical oxidation has also been demonstrated to effectively remediate dioxane and chlorinated solvents. Other in situ remedies, such as enhanced bioremediation, phytoremediation, and monitored natural attenuation, have been studied; however, their ability to achieve cleanup levels is still somewhat questionable and is limited by co‐occurring contaminants. This article summarizes and provides practical perspectives on dioxane analysis, plume stability relative to other contaminants, and the development of investigation tools and treatment technologies.

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Glyoxylate Metabolism Is a Key Feature of the Metabolic Degradation of 1,4-Dioxane by Pseudonocardia dioxanivorans Strain CB1190

Cited by 80 publications

References 54 publications

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Role of Glyoxylate Shunt in Oxidative Stress Response

Practical Perspectives of 1,4‐Dioxane Investigation and Remediation

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