Glycyrrhizin Suppresses the Expressions of HMGB1 and Relieves the Severity of Traumatic Pancreatitis in Rats

Abstract

BackgroundHigh mobility group box 1 (HMGB1) plays important roles in a large variety of diseases; glycyrrhizin (GL) is recognized as an HMGB1 inhibitor. However, few studies have focused on whether glycyrrhizin can potentially improve the outcome of traumatic pancreatitis (TP) by inhibiting HMGB1.MethodsA total of 60 male Wistar rats were randomly divided into three groups (n = 20 in each): Control group, TP group and TP-GL group. Pancreatic trauma was established with a custom-made biological impact machine-III, and GL was administered at 15 minutes after the accomplishment of operation. To determine survival rates during the first 7 days after injury, another 60 rats (n = 20 in each) were grouped and treated as mentioned above. At 24 hours of induction of TP, the histopathological changes in pancreas were evaluated and serum amylase levels were tested. Serum tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and HMGB1 were measured using enzyme linked immunosorbent assay. HMGB1 expressions in pancreas were measured using immunohistochemical staining, Western blot and Real-Time PCR analysis.ResultsSerum levels of HMGB1, TNF-α and IL-6 were increased dramatically in TP group at 24 hours after induction of TP. However, these indicators were reduced significantly by GL administration in TP-GL group comparing with TP group (P<0.05). Meanwhile, survival analysis showed that the seven-day survival rate in TP-GL group was significantly higher than that in TP group (85% versus 65%, P<0.05). GL treatment significantly decreased the pancreatic protein and mRNA expressions of HMGB1 and ameliorated the pancreatic injury in rats with TP.ConclusionsGlycyrrhizin might play an important role in improving survival rates and ameliorating pancreatic injury of TP by suppression of the expressions of HMGB1 and other proinflammatory cytokine.

Effects of silencing the DUSP1 gene using lentiviral vector-mediated siRNA on the release of proinflammatory cytokines through regulation of the MAPK signaling pathway in mice with acute pancreatitis

“…HMGB1 and S100A12, as mediators of inflammation, are considered to contribute to the proinflammatory response in acute respiratory distress syndrome (27). The results of a study performed by Xiang et al also demonstrated that there were increased serum levels of HMGB1, TNF-α and IL-6 in traumatic pancreatitis (28). Serum amylase is widely used as a biochemical marker of pancreatic inflammation (29).…”

PKR and HMGB1 expression and function in rheumatoid arthritis

“…HMGB1 is also implicated in other diseases, such as pancreatitis and cancer. Xiang et al (2014) suggested that inhibition of HMGB1 activity in patients with acute pancreatitis could improve clinical outcomes. Another study revealed that HMGB1 plays an important role in the process of malignant tumorigenesis by dysregulating cancer-related genes, promoting metastasis, and facilitating tumor growth and survival (Inoue et al, 2015).…”

Glycyrrhizin Attenuates Proinflammatory Cytokines through a Peroxisome Proliferator-Activated Receptor-&#947;-Dependent Mechanism and Experimental Vasospasm in a Rat Model

“…The dose was adjusted according to the studies of Xiang et al [41] and Li et al [42] on artery rings. The first injections given to animals were administered 24 h before (precondition) and 1 h after induction of SAH (treatment) by using an osmotic minipump (Alzet Corp., Palo Alto, Calif., USA).…”