Glycyrrhetinic acid prevents carbapenem-resistant Klebsiella pneumoniae-induced cell injury by inhibiting mitochondrial dysfunction via Nrf-2 pathway

Guan, Xiaodan; Jin, Liang; Yu, Dao‐Yi; Bao, Yida; Zhou, Huifen; Wan, Haitong

doi:10.1016/j.micpath.2022.105825

Cited by 5 publications

(5 citation statements)

References 28 publications

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“…The inflammation was reduced to some extent by glycyrrhizic acid treatment. Glycyrrhizic acid exerted oxidative homeostasis by activating the Nrf-2 signaling pathway to produce antioxidant proteins [ 26 ]. Recent studies also reported that lipopolysaccharide (LPS) enhanced apoptosis and promoted the production of inflammatory factors, such as TNF-α, IL-6, and IL-18 by the lung epithelial BEAS-2B cells, while amygdalin pretreatment counteracted the effect of LPS.…”

Section: Discussionmentioning

confidence: 99%

Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli

Xue

Jin

Zhou

et al. 2023

J. Microbiol. Biotechnol.

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Infectious diseases caused by drug-resistant Escherichia coli ( E. coli ) pose a critical concern for medical institutions as they can lead to high morbidity and mortality rates. In this study, amygdalin exhibited anti-inflammatory and antioxidant activities, as well as other potentials. However, whether it could influence the drug-resistant E. coli -infected cells remained unanswered. Amygdalin was therefore tested in a cellular model in which human macrophages were exposed to resistant E. coli . Apoptosis was measured by flow cytometry and the lactate dehydrogenase (LDH) assay. Western immunoblotting and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) were used to quantify interleukin-18 (IL-18), interleukin-1β (IL-1β), and interleukin-6 (IL-6). The production of reactive oxygen species (ROS) in macrophages was detected by ROS kit. The expression of panapoptotic proteins in macrophages was measured by qRT-PCR and Western immunoblotting. Drug-Resistant E. coli inhibited cell viability and enhanced apoptosis in the cellular model. In cells treated with amygdalin, this compound can inhibit cell apoptosis and reduce the expression of pro - inflammatory cytokines such as IL-1β, IL-18 and IL-6. Additionally, it decreases the production of PANoptosis proteins, Furthermore, amygdalin lowered the levels of reactive oxygen species induced by drug-resistant E. coli , in cells, demonstrating its antioxidant effects. Amygdalin, a drug with a protective role, alleviated cell damage caused by drug-resistant E. coli in human macrophages by inhibiting the PANoptosis signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli

Xue

Jin

Zhou

et al. 2023

J. Microbiol. Biotechnol.

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“…CRKP strain was isolated from Hangzhou First People's Hospital, and previous studies tested the resistance of CRKP to antibiotics 20 . CRKP strain was inoculated in LB broth under 200 rpm, 37 ℃.…”

Section: Methodsmentioning

confidence: 99%

“…In previous studies, we found that YHPG had a positive effect on the treatment of viral infectious respiratory diseases 14 . At the same time, recent studies had shown that oxidative stress played an important role in the pathogenesis of many inflammatory diseases and induced cell apoptosis and that high levels of reactive oxygen species produced by elements such as LPS and KP lead to oxidative stress 18 – 20 . PD had demonstrated to reduce inflammation and pulmonary fibrosis caused by Mycoplasma pneumoniae infection by blocking the NLRP3 inflammasome and NF-κB pathway 21 .…”

Section: Introductionmentioning

confidence: 99%

Polydatin prevent lung epithelial cell from Carbapenem-resistant Klebsiella pneumoniae injury by inhibiting biofilm formation and oxidative stress

Guan,

Jin,

Zhou

et al. 2023

Sci Rep

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Carbapenem-resistant Klebsiella pneumoniae (CRKP) causes severe inflammation in various infectious diseases, such as bloodstream infections, respiratory and urinary tract infections, which leads to high mortality. Polydatin (PD), an active ingredient of Yinhuapinggan granule, has attracted worldwide attention for its powerful antioxidant, anti-inflammatory, antitumor, and antibacterial capacity. However, very little is known about the effect of PD on CRKP. In this research, we evaluated the inhibitory effects of PD on both the bacterial level and the bacterial-cell co-culture level on anti-biofilm and efflux pumps and the other was the inhibitory effect on apoptosis, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) after CRKP induction. Additionally, we validated the mechanism of action by qRT-PCR and western blot in human lung epithelial cells. Firstly, PD was observed to have an inhibitory effect on the biofilm of CRKP and the efflux pump AcrAB-TolC. Mechanically, CRKP not only inhibited the activation of Nuclear Factor erythroid 2-Related Factor 2 (Nrf-2) but also increased the level of ROS in cells. These results showed that PD could inhibit ROS and activate Nrf-2 production. Together, our research demonstrated that PD inhibited bacterial biofilm formation and efflux pump AcrAB—TolC expression and inhibited CRKP-induced cell damage by regulating ROS and Nrf-2-regulated antioxidant pathways.

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“…64 65 Glycyrrhizic acid is one of the extracts of Gancao (Glycyrrhizae Radix et Rhizoma), with certain anticancer and antiviral effects. 66 Guan et al 67 showed that in the carbapenem-resistant Klebsiella pneumoniae (CRKP)-induced human lung epithelial cell model by activating Nrf2 expression glycyrrhizic acid can promote antioxidant protein production, inhibit the expression of inflammatory factors, and alleviate mitochondrial damage and cell apoptosis, thus reducing lung tissue damage caused by CRKP infection. Huanglian (Coptidis Rhizoma) mainly functions to clear heat, dry dampness, purge fire, and detoxify.…”

Section: Research Status Of Traditional Chinese Medicine Regulation O...mentioning

confidence: 99%

Research Progress on Chinese Medicine Regulation of Mitochondrial Damage for Intervention in Respiratory System Diseases

Li,

Yang,

Qin

et al. 2024

Chinese medicine and natural products

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Mitochondria are important organelles in cells for energy production, and mitochondrial damage caused by various reasons is an important factor promoting disease progression. Mitochondrial damage involves structural damage and mitochondrial DNA damage, which are closely related to the occurrence and development of respiratory system diseases. In recent years, a large number of studies have confirmed the significant role of mitochondrial damage in the progression of respiratory system diseases, which may be an important target for the treatment of respiratory system diseases with traditional Chinese medicine (TCM). This article reviews the role of TCM in regulating mitochondrial damage for intervention in respiratory system diseases such as chronic obstructive pulmonary disease, lung cancer, pulmonary fibrosis, acute lung injury, asthma, and pneumonia, aiming to provide a basis for the study of the pathogenesis and drug action targets of respiratory system diseases.

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Glycyrrhetinic acid prevents carbapenem-resistant Klebsiella pneumoniae-induced cell injury by inhibiting mitochondrial dysfunction via Nrf-2 pathway

Cited by 5 publications

References 28 publications

Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli

Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli

Polydatin prevent lung epithelial cell from Carbapenem-resistant Klebsiella pneumoniae injury by inhibiting biofilm formation and oxidative stress

Research Progress on Chinese Medicine Regulation of Mitochondrial Damage for Intervention in Respiratory System Diseases

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