Glycoxidized low-density lipoprotein regulates the expression of scavenger receptors in THP-1 macrophages

Lam, Michael C.; Tan, Kathryn Choon Beng; Lam, Karen S.L.

doi:10.1016/j.atherosclerosis.2004.08.003

Cited by 39 publications

(22 citation statements)

References 32 publications

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“…In parallel, the expression of SR-A, which also showed a tendency towards decreased levels in the high-AGE diet-fed group, seems to contribute to the decreased clearance of AGEs, thus promoting AGE-related complications. Although SR-A-related published data are still controversial since high glucose or modified LDL are able to increase the expression of SR-A (Lam et al 2004, Fukuhara-Takaki et al 2005, while TNFa is able to decrease it in macrophages (Hsu et al 1996), even these in vitro or in vivo data cannot be compared to the peripheral monocytic SR-A expression and have a lot of differences to the AGEs receptors' regulation in the dietary glycotoxins model that we performed here. Another novel finding of this study is the possible link between the expression of scavenger receptors, such as RAGE, and the hormonal dysregulation of the female reproductive system, since the monocytic expression of RAGE was negatively correlated with testosterone levels and positively correlated with progesterone levels.…”

Section: Discussionmentioning

confidence: 93%

Dietary glycotoxins affect scavenger receptor expression and the hormonal profile of female rats

Chatzigeorgiou¹,

Kandaraki²,

Piperi³

et al. 2013

Journal of Endocrinology

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The levels of advanced glycation end products (AGEs) are increased under conditions of impaired glucose metabolism and/or oxidative stress, promoting insulin resistance and other endocrine abnormalities. AGEs play a major role in the pathogenesis of several diseases such as diabetes, atherosclerosis, polycystic ovary syndrome and Alzheimer's disease, contributing to progressive ageing. Receptor-based clearance of AGEs by the receptor for AGE (RAGE) and/or the macrophage scavenger receptor A (SR-A) is considered as a main factor for the regulation of the concentration of AGEs under these conditions. This study aimed to investigate the expression of RAGE (AGER) and SR-A (MSR1) under high/low-dietary AGE conditions in vivo and their potential contribution to the metabolic and sex hormonal profile of female rats. Female Wistar rats were fed a low-AGE or high-AGE diet for 3 months. Serum samples were collected at baseline and at the completion of the 3-month period for the measurements of metabolic and hormonal parameters. Peripheral blood mononuclear cells (PBMCs) were isolated for the determination of the expression of RAGE and SR-A. The high-AGE diet-fed rats exhibited increased glucose, insulin and testosterone levels as well as decreased oestradiol and progesterone levels compared with the low-AGE diet-fed ones, thus indicating a metabolic and hormonal dysregulation attributed to high-AGE dietary exposure. The expression of RAGE was significantly down-regulated in the PBMCs of the high-AGE diet-fed rats (PZ0.041), and it was correlated negatively with insulin and testosterone levels and positively with progesterone levels. The expression of SR-A was also decreased in the high-AGE diet-fed rats to marginal significance. Decreased monocytic expression of scavenger receptors such as RAGE and SR-A may result in a higher deposition of AGEs in peripheral endocrine tissues, thus promoting endocrine-related abnormalities and diseases.

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Section: Discussionmentioning

confidence: 93%

Dietary glycotoxins affect scavenger receptor expression and the hormonal profile of female rats

Chatzigeorgiou¹,

Kandaraki²,

Piperi³

et al. 2013

Journal of Endocrinology

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“…Glucose is an important AGE precursor, but lipids, reactive oxygen species and amino acids might also significantly contribute to the complex process of AGE formation [7,8]. Previous in vitro studies revealed that both oxidised [9][10][11] and glycoxidised LDL [12] are able to increase CD36 mRNA expression in macrophages. Moreover, glycation of LDL, in the absence of oxidation, gives rise to lipid accumulation in macrophages [13].…”

Section: Introductionmentioning

confidence: 99%

Glycoxidised LDL isolated from subjects with impaired glucose tolerance increases CD36 and peroxisome proliferator–activator receptor γ gene expression in macrophages

et al. 2007

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Aims/hypothesis Glycoxidised LDL has been implicated in the pathogenesis of atherosclerosis, a major complication of diabetes. Since atherogenesis may occur at an early stage of diabetes, we investigated whether circulating LDL isolated from subjects with IGT (n=20) showed an increased glycoxidation status and explored the proatherogenic effects of LDL samples on macrophages. Subjects and methods We investigated LDL modifications using GC-MS. Murine macrophages were incubated with LDL samples for 1 h, and then mRNA expression rates of the scavenger receptors CD36 and scavenger receptor class B type 1 (SCARB1, formerly known as SR-BI) and transcription factor peroxisome proliferator-activator receptor γ (PPARγ) were quantified by real-time RT-PCR. Results The GC-MS experiments revealed that oxidative modifications of proline, arginine, lysine and tyrosine residues in apolipoprotein B100 were three-to fivefold higher in LDL samples from IGT subjects compared with those from NGT subjects (n=20). Moreover, LDL glycoxidation estimated by both N " -(carboxymethyl)lysine (CML) and N " -(carboxyethyl)lysine (CEL) residues was increased more than ninefold in LDL from IGT subjects compared with samples from NGT subjects. Compared with NGT LDL, IGT LDL elicited a significantly higher CD36 ( p<0.05) and PPARG ( p<0.05) gene expression, whereas SCARB1 mRNA expression was not affected. Conclusions/interpretation These data suggest that IGT is associated with increased glycoxidation of circulating LDL, which might contribute to the conversion of macrophages into a proatherogenic phenotype.

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“…Parece que en la diabetes, la glicación de las partículas de LDL son las que inician la formación de células espumosas (17,18) . Algunos investigadores han encontrado que los monocitos en sujetos con hiperglucemia tienen aumentada la expresión del receptor CD36 en comparación con aquellos sujetos con concentración normal de glucosa (19) .…”

Section: Introductionunclassified

sCD36 sérico y variables cardiometabólicas en dos poblaciones peruanas: Carhuamayo (4100 msnm) y Mala (30 msnm)

et al. 2017

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RESUMENObjetivo: Determinar los niveles séricos de sCD36, molécula relacionada al metabolismo lipídico, en poblaciones de la altura y del nivel del mar, y establecer la asociación de este parámetro con factores de riesgo cardiometabólico. Materiales y métodos: Participaron 45 personas de Carhuamayo (4100 msnm) y 40 personas de Mala (30 msnm). Se midió el peso, talla y presión arterial. Se determinó la hemoglobina en sangre total, y la glucosa, perfil lipídico y sCD36 en suero. Resultados: Se encontró en la población de Carhuamayo niveles de hemoglobina significativamente mayores, mientras que el peso, IMC y nivel de glucosa fueron significativamente menores que en Mala. No hubo diferencia significativa entre los niveles séricos de sCD36 de ambas poblaciones. Se observó una diferencia significativa entre los valores medios de sCD36 según el IMC, y una correlación positiva significativa entre sCD36 y el peso e IMC. Conclusiones: El nivel sérico observado de sCD36 es independiente de la altitud y puede ser considerado como marcador potencial de síndrome metabólico.Palabras clave: sCD36; factor de riesgo cardiometabólico; obesidad; altitud (Fuente: DeCS BIREME). Serum levels of sCD36 and cardiometabolic variables in two Peruvian towns: Carhuamayo (4100 masl) and Mala (30 masl)ABSTRACT Objectives: To determine the serum levels of sCD36, a lipid metabolism-related molecule, in high-altitude and sea-level populations, and to establish the association of this parameter with cardiometabolic risk factors. Materials and methods:The study population consisted of 45 people from Carhuamayo (4100 masl) and 40 people from Mala (30 masl). Weight, height and blood pressure were measured. Hemoglobin was determined in whole blood, and glucose, lipid profile and sCD36 in serum. Results: It has been found that hemoglobin levels in the population of Carhuamayo were significantly higher, while weight, BMI and glucose level were significantly lower than those in the population of Mala. There was no significant difference between serum levels of sCD36 in both populations. A significant difference was observed between sCD36 mean serum levels of both populations based on the BMI, and a significant positive correlation between sCD36 and the weight and BMI. Conclusions: The observed sCD36 serum level is not related to the altitude and can be considered as a potential marker of metabolic syndrome.

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Glycoxidized low-density lipoprotein regulates the expression of scavenger receptors in THP-1 macrophages

Cited by 39 publications

References 32 publications

Dietary glycotoxins affect scavenger receptor expression and the hormonal profile of female rats

Dietary glycotoxins affect scavenger receptor expression and the hormonal profile of female rats

Glycoxidised LDL isolated from subjects with impaired glucose tolerance increases CD36 and peroxisome proliferator–activator receptor γ gene expression in macrophages

sCD36 sérico y variables cardiometabólicas en dos poblaciones peruanas: Carhuamayo (4100 msnm) y Mala (30 msnm)

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