Glycosyltransferases and their Use in Assessing Oligosaccharide Structure and Structure‐Function Relationships

Ta, Beyer; Sadler, J. Evan; Ji, Rearick; Jc, Paulson; Rl, Hill

doi:10.1002/9780470122976.ch2

Cited by 106 publications

(16 citation statements)

References 448 publications

(252 reference statements)

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“…They act sequentially on the growing oligosaccharide as it moves along the lumen of the endomembrane system. This chapter will discuss some of the glycosyltransferases which make protein-bound Nglycans ( Asn-GlcNAc N-glycosidic linkage) (Beyer et al, 1981;Snider, 1984;Kornfeld and Kornfeld, 1985;Schachter, 1986Schachter, , 1995Hemming, 1995;Verbert, 1995) and 0-glycans (Ser/ Thr-GalNAc 0-glycosidic linkage) (Beyer et al , 1981;Sadler, 1984;Brockhausen, 1989, 1992;Brockhausen, 1995). The reactions catalyzed by these enzymes are:…”

Section: Introductionmentioning

confidence: 99%

Glycosyltransferases Involved inN–andO–Glycan Biosynthesis

Brockhausen¹,

Schachter²

1996

Glycosciences

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Section: Introductionmentioning

confidence: 99%

Glycosyltransferases Involved inN–andO–Glycan Biosynthesis

Brockhausen¹,

Schachter²

1996

Glycosciences

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“…This group of enzymes is mainly involved in the biosynthesis of O-and N-linked complex oligosaccharides of glycoproteins leading to the formation of a new glycosidic linkage (Beyer et al, 1981;Kleene & Berger, 1993;Montreuil et al, 1995;Breton et al, 2012;Hadley et al, 2014). Glycosylation, catalyzed by GTs and controlled by both the cell and the structure of the protein itself, proceeds in a stepwise manner.…”

Section: Introductionmentioning

confidence: 98%

Synthesis of potential inhibitors of glycosyltransferases representing UDP-GlcNAc

et al. 2015

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Based on rational design of the transition state analog inhibitors of glycosyltransferases, four model glycomimetics of this type, viz. benzyl 2-thio-α-D-fructofuranoside 1-diethylphosphate (XIa), its β-anomer (XIb), and their ethyl 2-thio analogs -α-anomer (XIIa) and β-anomer (XIIb), were synthesized. In addition, fourteen precursors arising during the synthesis of the desired final model compounds (XI and XII), partially or fully acetylated benzyl and/or ethyl 2-thiofructofuranoside 1-diethyl phosphates, were isolated and characterized with the aim to prepare complete series of glycomimetics, representing donor UDP-GlcNAc designated for biological assays on human GnT's, viz. GnT-I, Core2GnT, and GnT-V.

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“…Their analysis, therefore, is limited to mass spectrometric, amperometric, or refractive index detection methods [41][42][43]. Alternatively, labeled substrates can be used [29,44,45]. Radiolabeled or fluorescently labeled substrates are, however, not readily available on the market.…”

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confidence: 99%