Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions

Gutiérrez-Cabrera, Ana E.; Zandberg, Wesley F.; Zenteno, Edgar; Rodrı́guez, Mario H.; Espinoza, Bertha; Lowenberger, Carl

doi:10.1111/1744-7917.12579

Cited by 6 publications

(21 citation statements)

References 63 publications

(110 reference statements)

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“…Some proteins were detected by using a single technique [ 15 , 18 , 23 , 43 , 46 , 48 , 55 , 57 , 65 , 66 ], whilst up to four complementary techniques were used in combination to identify, quantify, detect the activity of, and demonstrate the presence of certain proteins [ 51 ] ( Figure 8 ).…”

Section: Resultsmentioning

confidence: 99%

“…SDS-PAGE electrophoretic separations of complex mixtures [ 7 , 12 , 31 , 56 , 61 ] and protein recognition by Western blot (WB) with specific antibodies and/or lectins [ 12 , 15 , 28 , 31 , 56 ] were conclusive tools for protein detection in five articles (see Figure 8 and the Supplementary Table S2 of Protein Compendium).…”

Section: Resultsmentioning

confidence: 99%

“…Two authors reported the detection of proteins and glycoproteins by using SDS-PAGE, WB, and lectin blots [ 12 , 15 ]. These techniques allowed the identification of proteins by molecular weight and by glycosylation type.…”

Section: Discussionmentioning

confidence: 99%

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Expression of Proteins, Glycoproteins, and Transcripts in the Guts of Fasting, Fed, and Trypanosoma cruzi-Infected Triatomines: A Systematic Review

Reynoso-Ducoing,

González-Rete,

Díaz

et al. 2023

Pathogens

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Chagas disease is caused by the hemoflagellate protozoan Trypanosoma cruzi. The main transmission mechanism for the parasite in endemic areas is contact with the feces of an infected triatomine bug. Part of the life cycle of T. cruzi occurs in the digestive tract of triatomines, where vector and parasite engage in a close interaction at a proteomic–molecular level. This interaction triggers replication and differentiation processes in the parasite that can affect its infectivity for the vertebrate host. With the aim of compiling and analyzing information from indexed publications on transcripts, proteins, and glycoproteins in the guts of fasting, fed, and T. cruzi-infected triatomines in the period 2000–2022, a systematic review was conducted following the PRISMA guidelines. Fifty-five original research articles retrieved from PubMed and ScienceDirect were selected; forty-four papers reported 1–26,946 transcripts, and twenty-one studies described 1–2603 peptides/proteins.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Expression of Proteins, Glycoproteins, and Transcripts in the Guts of Fasting, Fed, and Trypanosoma cruzi-Infected Triatomines: A Systematic Review

Reynoso-Ducoing,

González-Rete,

Díaz

et al. 2023

Pathogens

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“…Taxonomy is a fundamental science for the entomo-epidemiology of Chagas disease, because correctly classifying triatomines can assist in the activity of vector control programs [ 28 ]. Even though since 2014 the generic status of the species grouped in Meccus has been changed to Triatoma , several authors continued publishing articles using the Meccus nomenclature as valid [ 20 , 29 – 46 ] and, quite mistakenly, as Triatoma ( Meccus ) pallidipennis [ 47 – 49 ]—since Meccus after the genus Triatoma (between parentheses) represents a subgenus and, so far, there are no valid subgenera in the subfamily Triatominae.…”

Section: Resultsmentioning

confidence: 99%

Trends in taxonomy of Triatomini (Hemiptera, Reduviidae, Triatominae): reproductive compatibility reinforces the synonymization of Meccus Stål, 1859 with Triatoma Laporte, 1832

et al. 2021

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Background Meccus' taxonomy has been quite complex since the first species of this genus was described by Burmeister in 1835 as Conorhinus phyllosoma. In 1859 the species was transferred to the genus Meccus and in 1930 to Triatoma. However, in the twentieth century, the Meccus genus was revalidated (alteration corroborated by molecular studies) and, in the twenty-first century, through a comprehensive study including more sophisticated phylogenetic reconstruction methods, Meccus was again synonymous with Triatoma. Events of natural hybridization with production of fertile offspring have already been reported among sympatric species of the T. phyllosoma subcomplex, and experimental crosses demonstrated reproductive viability among practically all species of the T. phyllosoma subcomplex that were considered as belonging to the genus Meccus, as well as between these species and species of Triatoma. Based on the above, we carried out experimental crosses between T. longipennis (considered M. longipennis in some literature) and T. mopan (always considered as belonging to Triatoma) to evaluate the reproductive compatibility between species of the T. phyllosoma complex. In addition, we have grouped our results with information from the literature regarding crosses between species that were grouped in the genus Meccus with Triatoma, in order to discuss the importance of experimental crosses to confirm the generic reorganization of species. Results The crosses between T. mopan female and T. longipennis male resulted in viable offspring. The hatching of hybrids, even if only in one direction and/or at low frequency, demonstrates reproductive compatibility and homeology between the genomes of the parents. Conclusion Considering that intergeneric crosses usually do not result in viable offspring in Triatominae, the reproductive compatibility observed between the T. phyllosoma subcomplex species considered in the Meccus genus with species of the Triatoma genus shows that there is “intergeneric” genomic compatibility, which corroborates the generic reorganization of Meccus in Triatoma. Graphic Abstract

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“…These membranes also contain many different glycoproteins, suggested to interact with the flagellate. 42 …”

Section: Development Of Trypanosoma Cruzi In the Vector – Effects Of The Vectormentioning

confidence: 99%

An Update on the Knowledge of Parasite–Vector Interactions of Chagas Disease

Schaub¹

2021

RRTM

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This review focusses on the interactions between the etiologic agent of Chagas disease, Trypanosoma cruzi , and its triatomine vector. The flagellate mainly colonizes the intestinal tract of the insect. The effect of triatomines on trypanosomes is indicated by susceptibility and refractoriness phenomena that vary according to the combination of the strains. Other effects are apparent in the different regions of the gut. In the stomach, the majority of ingested blood trypomastigotes are killed while the remaining transform to round stages. In the small intestine, these develop into epimastigotes, the main replicative stage. In the rectum, the population density is the highest and is where the infectious stage develops, the metacyclic trypomastigote. In all regions of the gut, starvation and feeding of the triatomine affect T. cruzi . In the small intestine and rectum, starvation reduces the population density and more spheromastigotes develop. In the rectum, feeding after short-term starvation induces metacyclogenesis and after long-term starvation the development of specific cells, containing several nuclei, kinetoplasts and flagella. When considering the effects of T. cruzi on triatomines, the flagellate seems to be of low pathogenicity. However, during stressful periods, which are normal in natural populations, effects occur often on the behaviour, eg, in readiness to approach the host, the period of time before defecation, dispersal and aggregation. In nymphs, the duration of the different instars and the mortality rates increase, but this seems to be induced by repeated infections or blood quality by the feeding on infected hosts. Starvation resistance is often reduced by infection. Longevity and reproduction of adults is reduced, but only after infection with some strains of T. cruzi . Only components of the surface coat of blood trypomastigotes induce an immune reaction. However, this seems to act against gut bacteria and favours the development of T. cruzi.

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Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions

Cited by 6 publications

References 63 publications

Expression of Proteins, Glycoproteins, and Transcripts in the Guts of Fasting, Fed, and Trypanosoma cruzi-Infected Triatomines: A Systematic Review

Expression of Proteins, Glycoproteins, and Transcripts in the Guts of Fasting, Fed, and Trypanosoma cruzi-Infected Triatomines: A Systematic Review

Trends in taxonomy of Triatomini (Hemiptera, Reduviidae, Triatominae): reproductive compatibility reinforces the synonymization of Meccus Stål, 1859 with Triatoma Laporte, 1832

An Update on the Knowledge of Parasite–Vector Interactions of Chagas Disease

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