Glycosylation of uroplakins. Implications for bladder physiopathology

Kątnik-Prastowska, Iwona; Lis, Jolanta; Matejuk, Agata

doi:10.1007/s10719-014-9564-4

Cited by 34 publications

(38 citation statements)

References 113 publications

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“…These subunits are made by proteins (uroplakins, UPs), which serve as the major receptors for UPEC adherence to the host cell and are localized within plaques on the apical membranes of the mature umbrella cells (Veranic et al, 2004 ). There is a correlation between the glycosylation changes in UPs and the different pathological conditions of the urothelium such UTI and interstitial cystitis (Birder, 2005 ; Katnik-Prastowska et al, 2014 ; Habuka et al, 2015 ).…”

Section: The Bladder Epithelium Shows Self-defense Mechanisms Againstmentioning

confidence: 99%

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies

2017

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Urinary tract infections (UTIs) are one of the most common pathological conditions in both community and hospital settings. It has been estimated that about 150 million people worldwide develop UTI each year, with high social costs in terms of hospitalizations and medical expenses. Among the common uropathogens associated to UTIs development, UroPathogenic Escherichia coli (UPEC) is the primary cause. UPEC strains possess a plethora of both structural (as fimbriae, pili, curli, flagella) and secreted (toxins, iron-acquisition systems) virulence factors that contribute to their capacity to cause disease, although the ability to adhere to host epithelial cells in the urinary tract represents the most important determinant of pathogenicity. On the opposite side, the bladder epithelium shows a multifaceted array of host defenses including the urine flow and the secretion of antimicrobial substances, which represent useful tools to counteract bacterial infections. The fascinating and intricate dynamics between these players determine a complex interaction system that needs to be revealed. This review will focus on the most relevant components of UPEC arsenal of pathogenicity together with the major host responses to infection, the current approved treatment and the emergence of resistant UPEC strains, the vaccine strategies, the natural antimicrobial compounds along with innovative anti-adhesive and prophylactic approaches to prevent UTIs.

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Section: The Bladder Epithelium Shows Self-defense Mechanisms Againstmentioning

confidence: 99%

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies

2017

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“…Urea is one factor that can induce fimbriae synthesis, which may explain the presence of fimbriae on bacteria in urine (41). FimH binds uroplakin Ia, and the uroplakins — composed of uroplakin Ia, Ib, II, and IIIa subunits — cover 90% of the urothelium in a crystalline-like array (42). The uroplakin subunits are glycoproteins with either high mannose or complex N-glycans (42).…”

Section: Discussionmentioning

confidence: 99%

“…FimH binds uroplakin Ia, and the uroplakins — composed of uroplakin Ia, Ib, II, and IIIa subunits — cover 90% of the urothelium in a crystalline-like array (42). The uroplakin subunits are glycoproteins with either high mannose or complex N-glycans (42). If the mannose residues are not highly exposed, then fimbriated bacteria may move until a region is reached that has exposed mannose.…”

Section: Discussionmentioning

confidence: 99%

FIMBRIAE AND FLAGELLA MEDIATED SURFACE MOTILITY AND THE EFFECT OF GLUCOSE ON NONPATHOGENIC AND UROPATHOGENICESCHERICHIA COLI

Ambagaspitiye¹,

Sudarshan²,

Hogins³

et al. 2019

Preprint

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word count: 223 15 Text word count (including abstract and importance): 3965 16 17 2ABSTRACT 18 We characterized the surface motility of nonpathogenic and pathogenic E. coli strains 19 with respect to the appendage requirement, flagella versus fimbriae, and the glucose requirement. 20 Nonpathogenic lab strains exhibited either slow or fast surface movement. The slow strains 21 required type 1 fimbriae for movement, while the fast strains required flagella and had an 22 insertion in the flhDC promoter region. Surface movement of three uropathogenic E. coli 23 (UPEC) strains was fast and required flagella, but these strains did not have an insertion in the 24 flhDC promoter region. We assessed swimming motility as an indicator of flagella synthesis and 25 found that glucose inhibited swimming of the slow nonpathogenic strains but not of the fast 26 nonpathogenic or pathogenic strains. Fimbriae-based surface motility requires glucose, which 27 inhibits cyclic-AMP (cAMP) and flagella synthesis; therefore, we examined whether surface 28 motility required cAMP. The surface motility of a slow, fimbriae-dominant, nonpathogenic strain 29 did not require cAMP, which was expected because fimbriae synthesis does not require cAMP. 30In contrast, the surface motility of a faster, flagella-dominant, UPEC strain required cAMP, 31 which was unexpected because swarming was unaffected by the presence of glucose. Electron 32 microscopy verified the presence or absence of fimbriae or flagella. In summary, surface 33 motilities of the nonpathogenic and uropathogenic E. coli strains of this study differed in the 34 appendage used and the effects of glucose on flagella synthesis. 35 36 IMPORTANCE 37Uropathogenic Escherichia coli strains cause 80-90% of community-acquired urinary 38 tract infections, and recurrent urinary tract infections, which can last for years, and often become 39 antibiotic resistant. Urinary tract infections can be associated with intra-vesical lesions extending 40 3 from localized trigonitis/cystitis to widely distributed pancystitis: motility may be a factor that 41 distinguishes between these infection patterns. Nonpathogenic and uropathogenic E. coli were 42 shown to exhibit fimbriae-and flagella-dependent surface motility, respectively, and the 43 difference was attributed to altered control of flagella synthesis by glucose. Uropathogenic E. 44 coli strains grow more rapidly in urine than nonpathogenic strains, which implies differences in 45 metabolism. Understanding the basis for glucose-insensitive control of flagella-dependent 46 motility could provide insight into uropathogenic E. coli metabolism and virulence. 47 48 49 Escherichia coli is an extraordinarily successful pathogen which causes a variety of 50 diseases, including urinary tract infections (1). Uropathogenic E. coli (UPEC) is the predominant 51 cause of acute and recurrent urinary tract infections, which can become antibiotic resistant and 52 persist for years (2, 3). In women with recurrent urinary tract infections (rUTIs), the cla...

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“…18 Proper barrier function of urothelial cells depends on the precise formation of apical plasma membrane, which contains lipid composition and epithelium-specific glycoproteins called uroplakins. 19 The uroplakins of all mammalian urothelium comprise four major subunits, namely, uroplakins Ia and Ib, uroplakin II, and uroplakin IIIa, which synthesize a unique crystalline 2D tetramer of 16-nm particles. 19 Uroplakins, which are integral membrane proteins and integrated into plaques that possess a special shield to water and toxic molecules in urine, are synthesized in large numbers only by urothelial cells of terminally differentiated stage.…”

Section: Discussionmentioning

confidence: 99%

Tissue-engineered tubular substitutions for urinary diversion in a rabbit model

Meng

Liao

Yang

et al. 2015

Exp Biol Med (Maywood)

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Clinically, autologous gastrointestinal segments are traditionally used for urinary diversion. However, this procedure often causes many serious complications. Tissue engineering may provide an alternative treatment method in urinary diversion. This research aims to produce tissue-engineered tubular substitutions by using homologous adipose-derived stem cells, smooth muscle cells, and bladder acellular matrix in developing urinary diversion in a rabbit model. Adipose-derived stem cells and smooth muscle cells of rabbit were obtained and cultured in vitro. These cultured adipose-derived stem cells and smooth muscle cells were seeded onto the two sides of the bladder acellular matrix and then incubated for seven days. The cell-seeded matrix was used to build tissue-engineered tubular substitutions, which were then implanted and wrapped into the omentum in vivo for two weeks to promote angiogenesis. In the experimental group, the bladder of 20 rabbits was totally resected, and the above tissue-engineered tubular substitutions were used for urinary diversion. In the control group, bladder acellular matrix tubular substitutions with unseeded cells were implanted into the omentum and were used as urinary diversion on another five rabbits with the same process. The implants were harvested, and histological examination was conducted at 2, 4, 8, and 16 weeks after operation. Intravenous urography assessment was performed at 16 weeks postoperatively. All the rabbits were alive in the experimental group until they were sacrificed. Histological analysis of the construct displayed the presence of multilayer urothelial cells on the luminal side and organized smooth muscle tissue on the other side, and different diameters of neovascularization were clearly identified in the substitutions obtained. No leakage, stricture, or obstructions were noted with intravenous urography assessment. All the animals in the control group died within two weeks, and urine leakage, scar formation, and inflammation were detected through autopsy. This study demonstrates the feasibility of tissue-engineered tubular substitutions constructed using homologous adipose-derived stem cells, smooth muscle cells, and bladder acellular matrix for urinary diversion in a rabbit model.

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Glycosylation of uroplakins. Implications for bladder physiopathology

Cited by 34 publications

References 113 publications

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies

FIMBRIAE AND FLAGELLA MEDIATED SURFACE MOTILITY AND THE EFFECT OF GLUCOSE ON NONPATHOGENIC AND UROPATHOGENICESCHERICHIA COLI

Tissue-engineered tubular substitutions for urinary diversion in a rabbit model

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