Glycosylation of free trans-trans abscisic acid as a contributing factor in bud dormancy break

Leshem, Y.; Philosoph, Sonia; Wurzburger, Judith

doi:10.1016/0006-291x(74)90964-4

Cited by 27 publications

(6 citation statements)

References 3 publications

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“…PCR screening with vector specific primers confirmed the presence of UGT73C14 in 27 transgenic lines (data not shown). The glycosylated forms of ABA are considered to be biologically inactive [29]. Constitutive glycosylation of ABA in planta by overexpression of the UGT could lead to phenotypic changes typical for ABA deficiency.…”

Section: Resultsmentioning

confidence: 99%

A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)

et al. 2013

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Ligon lintless-2, a monogenic dominant cotton (Gossypium hirsutum L.) fiber mutation, causing extreme reduction in lint fiber length with no pleiotropic effects on vegetative growth, represents an excellent model system to study fiber elongation. A UDP-glycosyltransferase that was highly expressed in developing fibers of the mutant Ligon lintless-2 was isolated. The predicted amino acid sequence showed ~53% similarity with Arabidopsis UGT73C sub-family members and the UDP-glycosyltransferase was designated as UGT73C14. When expressed in Escherichia coli as a recombinant protein with a maltose binding protein tag, UGT73C14 displayed enzymatic activity toward ABA and utilized UDP-glucose and UDP-galactose as the sugar donors. The recombinant UGT73C14 converted natural occurring isoform (+)-cis, trans-ABA better than (+)-trans, trans-ABA and (-)-cis, trans-ABA. Transgenic Arabidopsis plants constitutively overexpressing UGT73C14 did not show phenotypic changes under standard growth conditions. However, the increased glycosylation of ABA resulted in phenotypic changes in post-germinative growth and seedling establishment, confirming in vivo activity of UGT73C14 for ABA. This suggests that the expression level of UGT73C14 is regulated by the observed elevated levels of ABA in developing fibers of the Li 2 mutant line and may be involved in the regulation of ABA homeostasis.

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Section: Resultsmentioning

confidence: 99%

A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)

et al. 2013

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“…Examination of endogenous levels has revealed that ABA content may decrease before bud burst (Emmerson & Powell 1978). Also, a decrease in the ratio of free to bound (inactive) forms of ABA may occur before bud break (Leshem et al 1974;Harrison & Saunders 1975;Wright 1975). In the current study, the application of exogenous ABA delayed bud break in the apices.…”

Section: Discussionmentioning

confidence: 99%

Effects of three plant growth regulators on growth, morphology, water relations, and frost resistance in lemonwood (Pittosporum eugenioidesA.Cunn)

Dwyer

Bannister

Jameson

1995

New Zealand Journal of Botany

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Pittosporum eugenioides is a native ornamental species with a wide distribution throughout New Zealand. The effects of the application of two plant hormones (gibberellic acid and ahscisic acid) and a gibberellin biosynthesis inhibitor (paclobutrazol) on the growth and development, frost resistance, and water loss on two-year old seedlings of P. eugenioides were studied. Gibberellic acid increased plant growth, stem diameter, and shoot succulence, while also inducing leaf hyponasty and vegetative bud break of apices. Abscisic acid induced growth suppression while being effective in promoting leaf epinasty and inhibiting bud break. Paclobutrazol induced growth suppression, promoted epinasty, decreased shoot succulence, inhibited bud break in apices, and increased foliar frost resistance. The effects of paclobutrazol were also overcome by the addition of gibberellic acid. The results are compared with those gained from species not native to New Zealand, and indicate the potential for the application of plant growth regulators to a New Zealand species of ornamental value.

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“…Bound ABA followed a similar pattern. Leshem et al (1974) noted a significant increase in bound ABA from December through February during the transition of almond buds {Prunus amygdalus cv. Pariche) from the dormant to the emergent state.…”

Section: Introductionmentioning

confidence: 99%

Seasonal Levels of Abscisic Acid in Buds and Stems of Acer saccharum

Dumbroff

Cohen

Webb

1979

Physiologia Plantarum

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Changes in abscisic acid (ABA) were followed in the buds and youngest stems of 6‐year‐old sugar maple (Acer saccharum Marsh.) saplings at approximately monthly intervals during the year. High levels present in the buds in July and August showed a moderate decline through early October, but subsequently increased following leaf fall and reached the maximum values detected during the study in November and December. Thereafter ABA fell sharply, reaching its lowest point immediately prior to budbreak in early May. While ABA in the stems followed a somewhat similar pattern, only minor changes were observed in the relatively low values detected between June and November. Except for the spring months, when concentrations of ABA in the stems and buds were at minimum levels, the buds always contained several times more inhibitor per unit dry weight of tissue. Analysis of selected samples from both tissues showed that bound (alkaline‐hydrolyzable) ABA increased during the overwintering season, with the highest values noted in the spring. Although the patterns of change observed in this study are indicative of a role for ABA in the overwintering process, ABA does not appear to be the primary endogenous factor responsible for initiation of the dormant state.

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Glycosylation of free trans-trans abscisic acid as a contributing factor in bud dormancy break

Cited by 27 publications

References 3 publications

A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)

A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)

Effects of three plant growth regulators on growth, morphology, water relations, and frost resistance in lemonwood (Pittosporum eugenioidesA.Cunn)

Seasonal Levels of Abscisic Acid in Buds and Stems of Acer saccharum

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