Glycosylation of DMP1 maintains cranial sutures in mice

Cai, Meili; Li, Junhui; Yue, Rui; Wang, Zuolin; Sun, Yao

doi:10.1111/joor.12881

Cited by 4 publications

(6 citation statements)

References 33 publications

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“…The junctures between the bones of the skull where the bones are held tightly together by fibrous tissue are called sutures. It has been reported that sutures are important for calvarial bone development and maintenance [ 31 ]. Approximately 85.5 ± 1.1% of the cells in the sutures possessed ARL13B-mCherry+ primary cilia, and the ciliary length varied from 5 to 7 μm ( Figure 5 H) ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Cilia in Different Mouse Tissues

Yang

Deepak

et al. 2021

Cells

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Cilia are microtubule-based hair-like organelles that extend from the cell surface. However, the existence and distribution of cilia in each organ and tissue at the postnatal stage in vivo remain largely unknown. In this study, we defined cilia distribution and arrangement and measured the ciliary lengths and the percentage of ciliated cells in different organs and tissues in vivo by using cilium dual reporter-expressing transgenic mice. Cilia were identified by the presence of ARL13B with an mCherry+ signal, and the cilium basal body was identified by the presence of Centrin2 with a GFP+ signal. Here, we provide in vivo evidence that chondrocytes and cells throughout bones have cilia. Most importantly, we reveal that: 1. primary cilia are present in hepatocytes; 2. no cilia but many centrioles are distributed on the apical cell surface in the gallbladder, intestine, and thyroid epithelia; 3. cilia on the cerebral cortex are well oriented, pointing to the center of the brain; 4. ARL13B+ inclusion is evident in the thyroid and islets of Langerhans; and 5. approximately 2% of cilia show irregular movement in nucleus pulposus extracellular fluid. This study reveals the existence and distribution of cilia and centrioles in different tissues and organs, and provides new insights for further comprehensive study of ciliary function in these organs and tissues.

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Section: Resultsmentioning

confidence: 99%

Identification of Cilia in Different Mouse Tissues

Yang

Deepak

et al. 2021

Cells

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“…Thinner cortical bone, decreased trabecular number and diminished osteocyte lacuna were detected in the S89G-DMP1 mice [11]. Interestingly, craniomaxillofacial deformities and cranial structure abnormalities were also clearly observed [16]. Additionally, our previous studies showed that DMP1-PG was highly expressed in the cartilage callus of fracture sites, and the S89G-DMP1 mouse fracture endochondral ossification model displayed abnormal chondrogenesis and delayed fracture healing.…”

Section: Introductionmentioning

confidence: 83%

“…DMP1-PG is a novel acidic ECM PG that is highly expressed in the bone matrix, cartilage and fracture callus. DMP1-PG deficiency could lead to abnormal craniomaxillofacial development and delayed fracture healing in long bones [16]. However, we still have limited knowledge about the special role of DMP1-PG in cranial defect repair.…”

Section: Discussionmentioning

confidence: 99%

The role of proteoglycan form of DMP1 in cranial repair

Liu

Niu

Zhou

et al. 2022

BMC Mol and Cell Biol

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Background The cranial region is a complex set of blood vessels, cartilage, nerves and soft tissues. The reconstruction of cranial defects caused by trauma, congenital defects and surgical procedures presents clinical challenges. Our previous data showed that deficiency of the proteoglycan (PG) form of dentin matrix protein 1 (DMP1-PG) could lead to abnormal cranial development. In addition, DMP1-PG was highly expressed in the cranial defect areas. The present study aimed to investigate the potential role of DMP1-PG in intramembranous ossification in cranial defect repair. Methods Mouse cranial defect models were established by using wild- type (WT) and DMP1-PG point mutation mice. Microcomputed tomography (micro-CT) and histological staining were performed to assess the extent of repair. Immunofluorescence assays and real-time quantitative polymerase chain reaction (RT‒qPCR) were applied to detect the differentially expressed osteogenic markers. RNA sequencing was performed to probe the molecular mechanism of DMP1-PG in regulating defect healing. Results A delayed healing process and an abnormal osteogenic capacity of primary osteoblasts were observed in DMP1-PG point mutation mice. Furthermore, impaired inflammatory signaling pathways were detected by using RNA transcription analysis of this model. Conclusions Our data indicate that DMP1-PG is an indispensable positive regulator during cranial defect healing.

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“…It has been previously shown that the loss of OPN results in impaired osteoclast activity, which leads to impaired bone resorption (88). A more recent publication has outlined that mechanical stress in the sagittal cranial suture of neonatal mice leads to modified expression of OPN, suggesting that OPN is active in the osteoblast differentiation process in cranial sutures (112). Another study has suggested that BSP and OPN have a functional overlap, as they have the potential to compensate for each other's absence in response to a hormonal challenge (113).…”

Section: Craniofacial Skeletonmentioning

confidence: 99%

The Role of SIBLING Proteins in Dental, Periodontal, and Craniofacial Development

Figueredo

Abdelhay²,

Gibson³

2022

Front. Dent. Med

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The majority of dental, periodontal, and craniofacial tissues are derived from the neural crest cells and ectoderm. Neural crest stem cells are pluripotent, capable of differentiating into a variety of cells. These cells can include osteoblasts, odontoblasts, cementoblasts, chondroblasts, and fibroblasts which are responsible for forming some of the tissues of the oral and craniofacial complex. The hard tissue forming cells deposit a matrix composed of collagen and non-collagenous proteins (NCPs) that later undergoes mineralization. The NCPs play a role in the mineralization of collagen. One such category of NCPs is the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. This family is composed of dentin sialophosphosprotein (DSPP), osteopontin (OPN), dentin matrix protein 1 (DMP1), bone sialoprotein (BSP), and matrix extracellular phosphoglycoprotein (MEPE). The SIBLING family is known to have regulatory effects in the mineralization process of collagen fibers and the maturation of hydroxyapatite crystals. It is well established that SIBLING proteins have critical roles in tooth development. Recent literature has described the expression and role of SIBLING proteins in other areas of the oral and craniofacial complex as well. The objective of the present literature review is to summarize and discuss the different roles the SIBLING proteins play in the development of dental, periodontal, and craniofacial tissues.

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Glycosylation of DMP1 maintains cranial sutures in mice

Cited by 4 publications

References 33 publications

Identification of Cilia in Different Mouse Tissues

Identification of Cilia in Different Mouse Tissues

The role of proteoglycan form of DMP1 in cranial repair

The Role of SIBLING Proteins in Dental, Periodontal, and Craniofacial Development

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