Glycosylation employing bio-systems: from enzymes to whole cells

Křen, Vladimı́r; Thiem, Joachim

doi:10.1039/cs9972600463

Cited by 161 publications

(74 citation statements)

References 41 publications

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“…These experimental data further exemplify the importance of enzyme libraries in obtaining speci®c biotransformations [for previous reports see, for instance, Kren and Thiem, 1997;WeignerovaÂ et al, 1999), because the described products could only be isolated because of the availability of a wide spectrum of enzymes from dierent sources. Although the yields were low, the reaction protocols were straightforward and the products could be isolated in just one step, thus avoiding chemical protection and deprotection reactions.…”

Section: Discussionmentioning

confidence: 80%

Exploitation of a library of α‐galactosidases for the synthesis of building blocks for glycopolymers

Casali

Tarantini²,

Riva³

et al. 2001

Biotech & Bioengineering

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After screening a library of fungal alpha-galactosidases for the synthesis of functionalized alkyl alpha-D-galactopyranosides, four enzymes (isolated from Aspergillus terreus CCM55, Aspergillus commune CCM 2969, Penicillium vinaceum CCM 2384, or Penicillium brasilianum 2155) proved to be suitable for these biotransformations. The effect of different concentrations of alcohol on activity and stability of these enzymes was investigated. After optimization of the reaction conditions, three galactose derivatives (allyl, 2-nitroethyl and 2-(2',2',2'-trifluoroacetamido)-ethyl alpha-D-galactopyranoside, 1a, 3a, and 4a, respectively), suitable for subsequent chemical polymerization, were synthesized using either the "reverse hydrolysis" or the "transglycosylation" protocols.

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Section: Discussionmentioning

confidence: 80%

Exploitation of a library of α‐galactosidases for the synthesis of building blocks for glycopolymers

Casali

Tarantini²,

Riva³

et al. 2001

Biotech & Bioengineering

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“…이런 문제점은 폴리페놀의 당화과정 (glycosylation)을 통하여 합성된 배당체를 이용하여 극복할 수 있다. 배당체의 합성은 화학적 방법으로 가능하지만, 이런 방법은 고온, 고압을 포함하는 조건이 필수적이고 합성된 아 노머(anomer) 물질의 분리 및 환경문제 등을 야기하여 [18] 최 근에는 생물체가 지니는 당 전이 능력을 갖는 효소를 이용한 당 전이 반응(transglycosylation)을 통하여 빠르고 쉽게 생산 하고 있다 [8,13] (Fig. 2).…”

Section: 서 론unclassified

Enzymatic Synthesis of Polyphenol Glycosides by Amylosucrase

Park¹,

Choi²,

Park³

et al. 2011

Journal of Life Science

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The capability of synthesizing polyphenol glycosides was examined using recombinant amylosucrase from the hyperthermophilic bacterium Deinococcus geothermalis. Based on the action mode of amylosucrase, sucrose and twenty-one polyphenols were used as a donor and acceptors respectively. The transglycosylation reaction by amylosucrase produced one or two major polyphenol glycosides depending on the type of polyphenols used. The synthesized polyphenol glycosides were detected by thin-layer chromatography. The structures of the newly synthesized polyphenol glycosides were predicted based on the transglycosylation mechanism of the enzyme. According to the acceptability of the polyphenols, the structural characteristics of polyphenol as an efficient acceptor were evaluated. The results indicate that amylosucrase is an efficient catalyst for the enzymatic synthesis of polyphenol glycosides, which have high potentials in food, cosmetics, and pharmaceutical industries.

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“…[34] A constraint to the use of isolated GTs for biocatalysis applications is the requirement of supplementary activated sugar donors, which are usually expensive and not available in large quantity. Several methods have been reported for in vitro regeneration of sugar donors; [1,33,35] however, the cost of these systems is still considered to be expensive for general large-scale applications.…”

Section: Plant Family 1 Gts As Biocatalystsmentioning

confidence: 99%

“…A comparison of the advantages among these two synthetic approaches has been reviewed by Køen and Thiem. [1] Enzymatic glycosylation usually employs glycosyltransferases, glycosidases or "glycosynthases". [2,3] Glycosidases typically catalyse the hydrolysis of glycosidic linkages.…”

Section: Glycosyltransferases: An Introductionmentioning

confidence: 99%

Plant Glycosyltransferases: Their Potential as Novel Biocatalysts

Lim

2005

Chemistry A European J

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The potential application of glycosyltransferases in glycoconjugate synthesis has attracted considerable interest from the biotechnology community in recent years. This concept article focuses on the current understanding of the chemistry of a family of plant enzymes capable of glycosylating small lipophilic molecules. These enzymes are discussed in terms of their regio- and enantioselective substrate recognition, sugar-donor selectivity and their utility as biocatalysts in whole-cell systems.

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Glycosylation employing bio-systems: from enzymes to whole cells

Cited by 161 publications

References 41 publications

Exploitation of a library of α‐galactosidases for the synthesis of building blocks for glycopolymers

Exploitation of a library of α‐galactosidases for the synthesis of building blocks for glycopolymers

Enzymatic Synthesis of Polyphenol Glycosides by Amylosucrase

Plant Glycosyltransferases: Their Potential as Novel Biocatalysts

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