Glycosylation-Dependent Cell Adhesion Molecule 1 (GlyCAM 1) Is Induced by Prolactin and Suppressed by Progesterone in Mammary Epithelium**This work was supported by National Institutes of Health.

Hou, Zhaoyuan; Bailey, Jason P.; Vomachka, Archie J.; Matsuda, Manabu; Lockefeer, Jason A.; Horseman, Nelson D.

doi:10.1210/endo.141.11.7795

Cited by 13 publications

(4 citation statements)

References 22 publications

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“…The digoxigenin‐labelled RNA probes were made using DIG RNA labeling kits (Roche, Indianapolis, IN). Hind limb muscle sections were processed and hybridization was performed as previously reported (Hou et al , 2000). Hybridized DIG‐RNA probes were detected with anti‐digoxigenin‐rhodamine, Fab fragments (Roche).…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels

et al. 2011

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Vasculogenesis, the in-situ assembly of angioblast or endothelial progenitor cells (EPCs), may persist into adult life, contributing to new blood vessel formation. However, EPCs are scattered throughout newly developed blood vessels and cannot be solely responsible for vascularization. Here, we identify an endothelial progenitor/stem-like population located at the inner surface of preexisting blood vessels using the Hoechst method in which stem cell populations are identified as side populations. This population is dormant in the steady state but possesses colony-forming ability, produces large numbers of endothelial cells (ECs) and when transplanted into ischaemic lesions, restores blood flow completely and reconstitutes de-novo long-term surviving blood vessels. Moreover, although surface markers of this population are very similar to conventional ECs, and they reside in the capillary endothelium sub-population, the gene expression profile is completely different. Our results suggest that this heterogeneity of stem-like ECs will lead to the identification of new targets for vascular regeneration therapy.

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Section: Methodsmentioning

confidence: 99%

Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels

et al. 2011

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“…40,43 GlyCAM-1 is not solely expressed in the mammary glands; it is also detected in the epithelial cells of lymph nodes, lungs, uterus, and cochlea. 47 In lymph nodes, GlyCAM-1 mediates lymphocyte trafficking, while in other tissues, the function remains unknown. In the mammary gland, GlyCAM-1 expression is regulated by progesterone and prolactin.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In the mammary gland, GlyCAM-1 expression is regulated by progesterone and prolactin. 47 Suggested functions for GlyCAM-1 included the inhibition of lipases, 48 acting as a lubricant, 12 or protection against mastitis, 49 possibly by its antibacterial properties. 13 In the immune system, GlyCAM-1 has been indicated in L-selectinmediated leukocyte rolling and trafficking.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In lymph nodes, GlyCAM-1 mediates lymphocyte trafficking, while in other tissues, the function remains unknown. In the mammary gland, GlyCAM-1 expression is regulated by progesterone and prolactin . Suggested functions for GlyCAM-1 included the inhibition of lipases, acting as a lubricant, or protection against mastitis, possibly by its antibacterial properties .…”

Section: Resultsmentioning

confidence: 99%

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In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile

Valk-Weeber

Deelman-Driessen

Dijkhuizen

et al. 2020

J. Agric. Food Chem.

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The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles. In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3). The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1. We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs. GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation. Signature glycans can be used to recognize individual proteins in the overall whey glycoprofile as well as for protein concentration estimations. Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.

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Functional genomics of endothelial cells treated with anti-angiogenic or angiopreventive drugs

Albini

Indraccolo

Noonan

et al. 2010

Clin Exp Metastasis

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Angiogenesis is a highly regulated physiological process that has been studied in considerable detail given its importance in several chronic pathologies. Many endogenous factors and hormones intervene in the regulation of angiogensis and classical as well as targeted drugs have been developed for its control. Angiogenesis inhibition has come off the bench and entered into clinical application for cancer therapy, particularly for metastatic disease. While the clinical benefit is currently in terms of months, preclinical data suggest that novel drugs and drug combinations could lead to substantial improvement. The many targets of endogenous angiogenesis inhibitors reflect the complexity of the process; in contrast, current clinical therapies mainly target the vascular endothelial growth factor system. Cancer chemopreventive compounds can retard tumor insurgence and delay or prevent metastasis and many of these molecules hinder angiogenesis, a mechanism that we termed angioprevention. Angiopreventive drugs appear to prevalently act through the inhibition of the pro-inflammatory and anti-apoptotic player NFkappaB, thus contrasting inflammation dependent angiogenesis. Relatively little is known concerning the effects of these angiogenesis inhibitors on gene expression of endothelial cells, the main target of many of these molecules. Here we provide an exhaustive list of anti-angiogenic molecules, and summarize their effects, where known, on the transcriptome and functional genomics of endothelial cells. The regulation of specific genes can be crucial to preventive or therapeutic intervention. Further, novel targets might help to circumvent resistance to anti-angiogenic therapy. The studies we review are relevant not only to cancer but also to other chronic degenerative diseases involving endothelial cells, such as cardiovascular disorders, diabetes, rheumatoid arthritis and retinopaties, as well as vessel aging.

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Glycosylation-Dependent Cell Adhesion Molecule 1 (GlyCAM 1) Is Induced by Prolactin and Suppressed by Progesterone in Mammary Epithelium**This work was supported by National Institutes of Health.

Cited by 13 publications

References 22 publications

Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels

Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels

In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile

Functional genomics of endothelial cells treated with anti-angiogenic or angiopreventive drugs

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