Glycosylation as new pharmacological strategies for diseases associated with excessive angiogenesis

Bousseau, Simon; Vergori, Luisa; Soleti, Raffaella; Lenaers, Guy; Martinez, Maria Carmen; Andriantsitohaina, Ramaroson

doi:10.1016/j.pharmthera.2018.06.003

Cited by 38 publications

(31 citation statements)

References 470 publications

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“…Angiogenesis involves the destruction of pre-existing cell connections, mitosis, sprouting of endothelial cells, and maturation of new capillaries. Throughout the process of angiogenesis, VEGF serves as the initiating signal and induces endothelial cell sprouting 33. MMP9 stimulates the release of VEGF and contributes to the angiogenic process 34.…”

Section: Discussionmentioning

confidence: 99%

Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway

Wu¹,

Ding²,

Li³

et al. 2019

Int. J. Biol. Sci.

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Random-pattern skin flaps are widely used to close defects in reconstructive and plastic surgeries; however, they are vulnerable to necrosis, particularly in the distal portion of the flap. Here, we examined the effects of metformin on flap survival and the mechanisms underlying these effects. Following metformin treatment, the survival area, blood flow, and number of microvessels present in skin flaps were increased on postoperative day 7, whereas tissue edema was reduced. In addition, metformin promoted angiogenesis, inhibited apoptosis, relieved oxidative stress, and increased autophagy in areas of ischemia; these effects were reversed by autophagy inhibitors 3-methyladenine (3MA) or chloroquine (CQ). Either 3MA or CQ reversed the metformin-induced increase in flap viability. Moreover, metformin also activated the AMPK-mTOR-TFEB signaling pathway in ischemic areas. Inhibitions of AMPK via Compound C (CC) or AMPK shRNA adeno-associated virus (AAV) vector resulted in the downregulation of the AMPK-mTOR-TFEB signaling pathway and autophagy level in metformin-treated flaps. Taken together, our findings suggest that metformin improves the survival of random-pattern skin flaps by enhancing angiogenesis and suppressing apoptosis and oxidative stress. These effects result from increased autophagy mediated by activation of the AMPK-mTOR-TFEB signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway

Wu¹,

Ding²,

Li³

et al. 2019

Int. J. Biol. Sci.

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“…N-Glycosylation is the specific bond of glycans to the amide residue of an asparagine in Asn-X-Ser/Thr sequence (X amino acid cannot be proline). O-Glycosylation is the specific and covalent bond of glycans onto oxygen of a Ser or a Thr residue [12,13]. N-and O-linked glycosylation are finely regulated by transcriptional control of glycosyltransferases and glycosidases and their accessibility to substrates but also by the spatial compartmentalization of components related to glycosylation machinery [13,14].…”

mentioning

confidence: 99%

“…O-Glycosylation is the specific and covalent bond of glycans onto oxygen of a Ser or a Thr residue [12,13]. N-and O-linked glycosylation are finely regulated by transcriptional control of glycosyltransferases and glycosidases and their accessibility to substrates but also by the spatial compartmentalization of components related to glycosylation machinery [13,14]. Aberrant glycosylation can occur in pathological conditions such as cancers [15,16] and inflammation [11,[17][18][19].…”

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confidence: 99%

Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis

et al. 2020

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Background: Serum protein glycosylation is an area of investigation in inflammatory arthritic disorders such as rheumatoid arthritis (RA). Indeed, some studies highlighted abnormalities of protein glycosylation in RA. Considering the numerous types of enzymes, monosaccharides and glycosidic linkages, glycosylation is one of the most complex post translational modifications. By this work, we started with a preliminary screening of glycoproteins in serum from RA patients and controls. Methods:In order to isolate glycoproteins from serum, lectin wheat germ agglutinin was used and quantitative differences between patients and controls were investigated by LC-MS/MS. Consequently, we focused our attention on two glycoproteins found in this explorative phase: corticosteroid-binding globulin (CBG) and lipopolysaccharidebinding protein (LBP). The subsequent validation with immunoassays was widened to a larger number of early RA (ERA) patients (n = 90) and well-matched healthy controls (n = 90). Results:We observed a significant reduction of CBG and LBP glycosylation in ERA patients compared with healthy controls. Further, after 12 months of treatment, glycosylated CBG and LBP levels increased both to values comparable to those of controls. In addition, these changes were correlated with clinical parameters. Conclusions:This study enables to observe that glycosylation changes of CBG and LBP are related to RA disease activity and its response to treatment.

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“…Angiogenesis is one of the critical events in the progression of DR. Hyperglycaemia may cause an imbalance between stimulators and inhibitors of angiogenesis, which further leads to the formation of new blood vessels [ 18 ]. These immature blood vessels are more prone to leakage and ultimately result in serious complications [ 19 ]. Antiangiogenic treatment provides significant improvements in disease conditions.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy

Lhamo

Zou

et al. 2020

BMC Med Genomics

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Background Angiogenesis is an important parameter in the development of diabetic retinopathy (DR), and it is indicative of an early stage evolving into a late phase. Therefore, examining the role of angiogenic factors in early DR is crucial to understanding the mechanism of neovascularization. Methods The present study identified hub genes and pathways associated with angiogenesis in early DR using bioinformatics analysis. Genes from published literature and Gene Expression Omnibus (GEO) were collected and analysed. Results We collected 73 genes from 70 published studies in PubMed, which were referred to as DR-related gene set 1 (DRgset1). The gene expression profile of GSE12610 was downloaded, and 578 differentially expressed genes (DEGs) between diabetic and normal samples were identified. DEGs and DRgset1 were further combined to create DR-related gene set 2 (DRgset2). After an enrichment analysis, we identified 12 GO terms and 2 pathways associated with neovascularization in DRgset1, and 8 GO terms and 2 pathways in DRgset2. We found 39 new genes associated with angiogenesis and verified 8 candidate angiogenesis-related genes in DR cells using real-time PCR: PIK3CB, ALDH3A1, ITGA7, FGF23, THBS1, COL1A1, MAPK13, and AIF1. We identified 10 hub genes associated with neovascularization by constructing a protein-protein interaction (PPI) network: TNF, VEGFA, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1. Conclusions The present study analysed angiogenesis-related genes and pathways in early DR in a comprehensive and systematic manner. PIK3CB, ALDH3A1, ITGA7, FGF23, THBS1, COL1A1, MAPK13, and AIF1 may be the candidate genes to further explore the mechanisms of angiogenesis in early DR. TNF, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1 may be new targets for early neovascularization therapy in the future.

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Glycosylation as new pharmacological strategies for diseases associated with excessive angiogenesis

Cited by 38 publications

References 470 publications

Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway

Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway

Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis

Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy

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