Glycosylation, amino acid analysis and kinetic properties of a major Kunitz-type trypsin inhibitor from Acacia victoriae Bentham seeds

Ee, Kah Yaw; Zhao, Jian; Rehman, Aziz Ur; Agboola, Samson

doi:10.1016/j.foodchem.2011.05.026

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…Conversely, the low antioxidant activity of PIs, including that from wattle seed, could be due to the globular nature of the seed protein where the hydrophobic side chains of aromatic amino acids, such as phenylalanine, tyrosine, tryptophan and histidine, were not usually exposed. Furthermore, the low level of aromatic amino acids in AvTI, as reported in our previous study (Ee et al. , 2011b), was possibly another negative factor for the antioxidant properties.…”

Section: Resultssupporting

confidence: 62%

“…2), these biological analyses were not performed on roasted samples. The relatively low versatility of the wattle seed extracts in terms of their biological activities may be related to the low diversity of the phenolic constituents, as our previous study showed that phenolics in wattle seed were dominated by two phenolic acids, namely succinic and gallic acids (Ee et al. , 2011b).…”

Section: Resultsmentioning

confidence: 99%

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In vitro antioxidant and bioactive properties of raw and roasted wattle (Acacia victoriae Bentham) seed extracts

Agboola

Rehman³

et al. 2012

Int J of Food Sci Tech

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Protease inhibitors (PIs) from raw wattle (Acacia victoriae) seeds as well as phenolic extracts from raw and roasted wattle seeds were analysed for antioxidant, angiotensin-converting enzyme (ACE) inhibition, cytotoxicity, antimicrobial, antiparasitic, antitumor and insecticidal activities. Progressive purification of the PIs resulted in increases in their ACE inhibitory activity and cytotoxicity, but decreases in antioxidant activity. Roasting of wattle seed significantly increased the antioxidant activity of the phenolic extracts as did alkaline hydrolysis of wattle seed flour, but the treatment destroyed their ACE inhibitory activity and reduced the level of cytotoxicity. A moderate degree of synergism between the PIs and phenolic extracts of wattle seed was observed for ACE inhibition activity, but not for antioxidant activity or cytotoxicity. Wattle seed PIs exhibited moderate cytotoxicity, but did not possess any significant antibacterial, antifungal, antiparasitic, antitumour or insecticidal activities.Gel filtration fraction. e Data in column with the same superscripts are not significantly different, while data without superscripts are significantly different at 95% confidence level.Bioactive properties of wattle seed extracts K. Y. Ee et al.

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Section: Resultssupporting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

In vitro antioxidant and bioactive properties of raw and roasted wattle (Acacia victoriae Bentham) seed extracts

Agboola

Rehman³

et al. 2012

Int J of Food Sci Tech

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“…However, there are reports of trypsin inhibitors with covalently linked carbohydrates to their structures. For example, the trypsin inhibitor from Acacia victoriae (AvTI) has a glycosylation degree of 2.06% (Ee et al, 2011). The protease inhibitors of Echinodorus paniculatus (Paiva et al, 2003) and Peltophorum dubium (Macedo et al, 2003) are all glycosylated proteins.…”

Section: Assessment Of the Glycoprotein Nature And Amino-terminal Anamentioning

confidence: 99%

Castor bean cake contains a trypsin inhibitor that displays antifungal activity against Colletotrichum gloeosporioides and inhibits the midgut proteases of the dengue mosquito larvae

Silva

Vasconcelos

Filho

et al. 2015

Industrial Crops and Products

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“…However, the most widely studied is the serine protease inhibitors, which are grouped into 18 families based on their primary and three-dimensional structures, and mechanism of inhibition . Furthermore, plant serine protease inhibitors are divided into Kunitz, Bowman–Birk, Potato I, Potato II, and Squash families based mainly on their primary structure. , Generally, Bowman–Birk inhibitors are proteins of 8–10 kDa and have seven disulfide bonds and two independent reactive domains, one for trypsin and the other for chymotrypsin …”

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confidence: 99%

A Bowman–Birk Inhibitor from the Seeds of Luetzelburgia auriculata Inhibits Staphylococcus aureus Growth by Promoting Severe Cell Membrane Damage

et al. 2018

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Staphylococcus aureus is a multidrug-resistant bacterium responsible for several cases of hospital-acquired infections, which constitute a global public health problem. The introduction of new healthcare strategies and/or the discovery of molecules capable of inhibiting the growth or killing S. aureus would have a huge impact on the treatment of S. aureus-mediated diseases. Herein, a Bowman-Birk protease inhibitor ( LzaBBI), with strong in vitro antibacterial activity against S. aureus, was purified to homogeneity from Luetzelburgia auriculata seeds. LzaBBI in its native form is a 14.3 kDa protein and has a pI of 4.54, and its NH-terminal sequence has high identity with other Bowman-Birk inhibitors. LzaBBI showed a mixed-type inhibitory activity against both trypsin and chymotrypsin, respectively, and it remained stable after both boiling at 98 °C for 120 min and incubation at various pHs. Scanning electron microscopy revealed that LzaBBI disrupted the S. aureus membrane integrity, leading to bacterial death. This study suggests that LzaBBI is a powerful candidate for developing a new antimicrobial to overcome drug resistance toward reducing hospital-acquired infections caused by S. aureus.

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Glycosylation, amino acid analysis and kinetic properties of a major Kunitz-type trypsin inhibitor from Acacia victoriae Bentham seeds

Cited by 9 publications

References 26 publications

In vitro antioxidant and bioactive properties of raw and roasted wattle (Acacia victoriae Bentham) seed extracts

In vitro antioxidant and bioactive properties of raw and roasted wattle (Acacia victoriae Bentham) seed extracts

Castor bean cake contains a trypsin inhibitor that displays antifungal activity against Colletotrichum gloeosporioides and inhibits the midgut proteases of the dengue mosquito larvae

A Bowman–Birk Inhibitor from the Seeds of Luetzelburgia auriculata Inhibits Staphylococcus aureus Growth by Promoting Severe Cell Membrane Damage

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