Glycosphingolipids * *Sialic acid-containing glycosphingolipids (the gangliosides) are discussed in a separate chapter (see Chapter 3).

Makita, Akira; Taniguchi, Naoyuki

doi:10.1016/s0167-7306(08)60020-4

Cited by 39 publications

(16 citation statements)

References 658 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The poor immunogenicity of ganglioside antigen such as ganglioside GM1 is well documented [14]. In the present study, oral immunization of stable liposomes containing ganglioside GM1 in mice produced a serum IgA antibody response to ganglioside GM1, whereas that of ganglioside GM1 alone was unable to induce any detectable serum IgA response ( Table 2, Groups A and D), suggesting that stable liposomes possess innate adjuvant properties.…”

Section: Discussionsupporting

confidence: 64%

Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes after Oral Immunization.

Han

Watarai

Kobayashi

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. In order to evaluate the usefulness of liposomes as oral vaccines, the stability of liposomes and serum IgA antibody response to antigen associated with liposomes after oral administration were examined. Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS), and cholesterol (Chol) (1:1:2, molar ratio), distearoylphosphatidylcholine (DSPC) and Chol (7:2, molar ratio), and DSPC, DPPS, and Chol (7:3:2 or 1:1:2, molar ratio) were stable in acidic solution (pH 2.0), bile, and pancreatin solution, whereas liposomes composed of DPPC and Chol (7:2, molar ratio) and DPPC, DPPS, and Chol (7:3:2, molar ratio) were unstable in pH 2.0 and/or bile solutions. After the oral immunization of antigen (ganglioside GM1)-containing liposomes composed of DPPC, DPPS, and Chol (1:1:2, molar ratio) to mice, the serum IgA antibody responses against ganglioside GM1 were found. Furthermore, when monophosphoryl lipid A was incorporated into liposomes containing ganglioside GM1, further augmentation of IgA responses to ganglioside GM1 was observed. On the other hand, the oral administration with liposomes composed of DPPC, Chol, and ganglioside GM1 (unstable liposomes), ganglioside GM1 mixed with liposomes composed of DPPC, DPPS and Chol, and ganglioside GM1 alone was unable to induce any detectable anti-ganglioside GM1 IgA antibody responses. These results suggest that liposomes which showed the stability to acidic solution, bile, and pancreatin solution would serve effectively as an oral delivery vehicle for inducing mucosal immune responses. -KEY WORDS: liposome, mucosal immunity, oral administration, oral vaccine, stability.

show abstract

Section: Discussionsupporting

confidence: 64%

Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes after Oral Immunization.

Han

Watarai

Kobayashi

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…In plants and yeast, the headgroup is phosphoinositol (inositolphosphoceramide, IPC), often glycosylated and otherwise extended, which in yeast results in mannosyl-inositolphosphoceramide (MIPC), and inositolphosphomannosyl-inositolphosphoceramide (M(IP) 2 C). However, a multitude of other headgroups have been identi¢ed in the various eukaryotes and even in some bacteria [79,156] is only relevant between cytosolic membrane lea£ets. So far, there is no evidence that compartments exchange lipids via hemi-fusion, whereby lipids would di¡use from the one compartment to the other via a continuous cytosolic membrane lea£et.…”

Section: Possible Mechanisms Of Intracellular Sphingolipid Transportmentioning

confidence: 99%

“…LacCer in the lumen of the Golgi can serve as a substrate for glycosyltransferases, each of which gives rise to a trihexosylceramide that is the precursor of a speci¢c series of glycosphingolipids assembled by the stepwise addition of carbohydrate residues [156]. Most cells synthesize the ganglioside GM3, by the transfer of sialic acid to LacCer [1571 59], but in addition make one other trihexosylceramide.…”

Section: Glycosphingolipidsmentioning

confidence: 99%

Sphingolipid transport in eukaryotic cells

Meer

Holthuis

2000

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

153

View full text Add to dashboard Cite

Sphingolipids constitute a sizeable fraction of the membrane lipids in all eukaryotes and are indispensable for eukaryotic life. First of all, the involvement of sphingolipids in organizing the lateral domain structure of membranes appears essential for processes like protein sorting and membrane signaling. In addition, recognition events between complex glycosphingolipids and glycoproteins are thought to be required for tissue differentiation in higher eukaryotes and for other specific cell interactions. Finally, upon certain stimuli like stress or receptor activation, sphingolipids give rise to a variety of second messengers with effects on cellular homeostasis. All sphingolipid actions are governed by their local concentration. The intricate control of their intracellular topology by the proteins responsible for their synthesis, hydrolysis and intracellular transport is the topic of this review. ß

show abstract

“…The level of Gb3 in group D was slightly lower than that in group A, whereas the Gb4 content was higher in group D than in group A (P Ͻ 0.007). Gb4 is synthesized from Gb3 by the enzyme ␤-N-acetylgalactosaminyl transferase (13). Thus, the differences in serum Gb4 levels between group A and group D might be explained by differences in expression of this enzyme between the HUS patients in the acute phase and those in the convalescent phase.…”

mentioning

confidence: 99%

Relationship between Susceptibility to Hemolytic-Uremic Syndrome and Levels of Globotriaosylceramide in Human Sera

et al. 2001

View full text Add to dashboard Cite

The relationship between differential susceptibility to hemolytic-uremic syndrome (HUS) and levels of globotriaosylceramide (Gb3) in serum was studied in patients infected with verotoxin-producing Escherichia coli (VTEC). The serum Gb3 levels in patients with HUS were lower than these in diarrheal patients without subsequent HUS or in patients without clinical symptoms, indicating that individuals with a lower content of serum Gb3 show a higher incidence of HUS following VTEC infection

show abstract

Glycosphingolipids * *Sialic acid-containing glycosphingolipids (the gangliosides) are discussed in a separate chapter (see Chapter 3).

Cited by 39 publications

References 658 publications

Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes after Oral Immunization.

Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes after Oral Immunization.

Sphingolipid transport in eukaryotic cells

Relationship between Susceptibility to Hemolytic-Uremic Syndrome and Levels of Globotriaosylceramide in Human Sera

Contact Info

Product

Resources

About