Glycosphingolipids and antitumor immunity

Dyatlovitskaya, É. V.; Bergel'son, L. D.

doi:10.1016/0304-419x(87)90002-3

Cited by 39 publications

(27 citation statements)

References 134 publications

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“…They often recognize pathogens through a wide array of molecules such as (glyco)lipids and acylated proteins or peptides. Interestingly, several studies indicate that glycolipids are capable to modulate the human immune system (45)(46)(47). The presence of lipid moieties within pathogen-derived products is essential for activation of specific pattern recognition receptors, in particular TLR2 (48).…”

Section: Discussionmentioning

confidence: 99%

DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN

Meyer

Liempt

Imberty

et al. 2005

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN

Meyer

Liempt

Imberty

et al. 2005

Journal of Biological Chemistry

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“…Thus, gangliosides inhibit the in vitro proliferative response of various classes of activated immune cells such as T-and B-lymphocytes, macrophages, and natural killer cells (52). However, the molecular mechanism(s) underlying the immunosuppressive activity of glycosphingolipids are incompletely understood but include the direct interaction of ganglioside micelles with IL-2 and IL-4 in the modulation of IL-2-/IL-4-dependent processes (53) and the interference of monocytes at the level of antigen presentation (54).…”

Section: Discussionmentioning

confidence: 99%

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Lochnit¹,

Dennis²,

Ulmer³

et al. 1998

Journal of Biological Chemistry

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The isolated neutral glycosphingolipid fraction from the pig parasitic nematode, Ascaris suum, was fractionated by silica gel chromatography to yield a neutral and a zwitterionic glycosphingolipid fraction, the latter of which mainly contained two zwitterionic glycosphingolipids termed components A and C. Preliminary chemical characterization with hydrofluoric acid treatment and immunochemical characterization with a phosphocholine-specific monoclonal antibody indicated that both components contained phosphodiester substitutions: phosphocholine for component A, and phosphocholine and phosphoethanolamine for component C. Both components were biologically active in inducing human peripheral blood mononuclear cells to release the inflammatory monokines tumor necrosis factor ␣, interleukin 1, and interleukin 6. Component A was the more bioactive molecule, and its biological activity was abolished on removal of the phosphocholine substituent by hydrofluoric acid. The glycosphingolipid components were structurally analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, liquid secondary ion mass spectrometry, methylation analysis, 1 H NMR spectroscopy, exoglycosidase cleavage, and ceramide analysis. Their chemical structures were elucidated to be (see Structure I below), The carbohydrate moiety oligosaccharide core was characterized as belonging to the arthro series of protostomial glycosphingolipids. The ceramide moiety was distinguished by (R)-2-hydroxytetracosanoic acid as the dominant fatty acid species and by the C17 iso-branched sphingosine and sphinganine bases, 15-methylhexadecasphing-4-enine and 15-methylhexadecasphinganine, respectively.Analyses of the immunoreactivity between neutral fraction glycolipids derived from various species of parasitic nematodes have indicated a high degree of serological cross-reactivity (1, 2). The structural basis for this immunological cross-reactivity between parasitic nematodes at the level of glycolipids is at present unknown. Structural studies on the neutral fraction glycosphingolipids from adults of the porcine parasitic nematode Ascaris suum have revealed that the identified arthro series oligosaccharide chain was not immunogenic, i.e. did not exhibit immunoreactivity toward infection sera from A. suuminfected mice (2, 3). However, a zwitterionic glycosphingolipid fraction was also isolated from A. suum that demonstrated a phosphodiester sidechain as a structural modification of possibly phosphocholine (PC) 1 and phosphoethanolamine (PE). In addition, these zwitterionic glycolipids were immunogenic/antigenic, i.e. exhibited immunoreactivity toward infection sera from A. suum-infected mice (2).PC-containing macromolecules have been regularly detected * This project was supported by the Deutsche Forschungsgemeinschaft (SFB 535 and Graduiertenkolleg Molecular Biology and Pharmacology) and by the Bundesministerium fü r Bildung, Wissenschaft, Forschung und Technologie Grant 01 KI 9471. The costs of publication of this article were defrayed in...

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“…Although glycosphingolipids are small molecules compared to proteins and account mostly for only a few percent of the total cell lipids, they comprise 30-60% of the lipid molecules on the outer half of the plasma membrane, forming a sort of sugar lawn on the cell surface, and play important roles in cell differentiation, morphogenesis, and oncogenic transformation [21]. Formation of clusters or aggregation of trace amounts of glycolipids on the cell membrane surface are considered to be important in regulating the structure and function of many membrane proteins.…”

Section: Gm3 Markedly Increases the Activity Of Ca2+-atpase Inmentioning

confidence: 99%

Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca²⁺‐ATPase

Wang

Yang

et al. 1996

FEBS Letters

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Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography, to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca z+ uptake activity of sarcoplasmic 2+ reticulum Ca -ATPase incorporated into the proteoliposomes. 2+Conformation changes of Ca -ATPase induced by GM3 were also observed by intrinsic fluorescence and circular dichroism measurements.

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Glycosphingolipids and antitumor immunity

Cited by 39 publications

References 134 publications

DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN

DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca²⁺‐ATPase

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Glycosphingolipids and antitumor immunity

Cited by 39 publications

References 134 publications

DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN

DC-SIGN Mediates Binding of Dendritic Cells to Authentic Pseudo-LewisY Glycolipids of Schistosoma mansoni Cercariae, the First Parasite-specific Ligand of DC-SIGN

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca2+‐ATPase

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Effect of ganglioside GM3 on the activity and conformation of reconstituted Ca²⁺‐ATPase