Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi

Solanki, Vipul; Krüger, Karen; Crawford, Cynda; Pardo-Vargas, Alonso; Danglad‐Flores, José; Hoang, Kim Le Mai; Klassen, Leeann; Abbott, D. Wade; Seeberger, Peter H.; Amann, Rudolf; Teeling, Hanno; Hehemann, Jan‐Hendrik

doi:10.1038/s41396-022-01223-w

Cited by 8 publications

(5 citation statements)

References 99 publications

(126 reference statements)

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“…Recent research also showed the degradation of fungal a-mannan by a Salegentibacter sp. (Bacteroidota) strain isolated during a phytoplankton bloom in the North Sea (Solanki et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Abundance and composition of particles and their attached microbiomes along an Atlantic Meridional Transect

Reintjes

Heins²,

Wang³

et al. 2023

Front. Mar. Sci.

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Particulate organic matter plays a significant role in the marine carbon cycle. Its sinking exports organic carbon from the surface to deep oceans. Using fractionated filtration, we analysed particles of 3 – 10 µm and >10 µm and their microbiomes in thirty-five stations along a latitudinal transect of the Atlantic Ocean and provide new insights into the composition, community dynamics, and catabolic potential of particle-attached bacteria. Samples were taken during an Atlantic Meridional Transect (AMT22), which traversed six distinctive ocean provinces. Using 16S rRNA amplicon sequencing and fluorescence in situ hybridisation, we could show a strong variation between particle-attached and free-living bacterial communities at each station and across the biogeographical provinces – a dynamic likely driven by chlorophyll a concentrations, temperature, and the oxygen content of the respective biogeographical provinces. Whereas the <3 µm fraction was primarily composed of SAR11, SAR86, Prochlorococcus and Bacteroidetes of the NS9 and NS5 clades, particle-attached communities were dominated by other Bacteroidetes (Polaribacter spp.), diverse Gammaproteobacteria including members of the genera Alteromonas and Vibrio, Alphaproteobacteria, Planctomycetes, OM27 and Verrucomicrobia. In three provinces, we quantified particle abundance and analysed their glycan composition using four lectins targeting fucose, galactose, N-acetylgalactosamine and mannose. Particles were mainly composed of fucose glycans with only a minor abundance of the other glycans, and particle abundance was directly correlated with the chlorophyll a concentrations. Functional analysis of 54 metagenome-assembled genomes retrieved from bacterial communities attached to small particles showed that particle-attached Bacteroidetes, Planctomycetes and Verrucomicrobia displayed key roles in the degradation of sulfated fucose-containing polysaccharides. We also identified gene clusters potentially encoding the utilisation of mannan and laminarin, suggesting an adaptation to the glycan composition of the particles, potentially resulting in niche diversification. Together, our results provide insights into particle-attached bacteria and their ecological strategies in the Atlantic.

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“…Recent research also showed the degradation of fungal a-mannan by a Salegentibacter sp. (Bacteroidota) strain isolated during a phytoplankton bloom in the North Sea (Solanki et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Abundance and composition of particles and their attached microbiomes along an Atlantic Meridional Transect

Reintjes

Heins²,

Wang³

et al. 2023

Front. Mar. Sci.

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“…For example, the common gut resident Bacteroides thetaiotaomicron dedicates ~7% of its genome to glycoside hydrolases (Kaoutari et al ., 2013). While CAZymes have been studied in detail within various commensal microbes and marine ecosystems (Sichert et al ., 2020; Solanki et al ., 2022), the role these enzymes play in pathogenic bacteria are still unclear. Interestingly, chitinases, which hydrolyze chitin polymers into N-acetyl glucosamine oligomers, are a well-studied family of glycoside hydrolases in pathogens.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics reveal Salmonella-liberated dietary L-arabinose promotes expansion in superspreaders

Ruddle

Massis

Cutter

et al. 2022

Preprint

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SUMMARYOur understanding of specific metabolites that drive host-pathogen interactions in the guts of superspreader hosts is incomplete. In a mouse model of chronic, asymptomatic Salmonella enterica serovar Typhimurium (S. Tm) infection, we performed untargeted metabolomics on the feces of mice and found superspreader hosts possess distinct metabolic signatures compared to non-superspreaders, including differential levels of L-arabinose. RNA-seq on S. Tm in superspreader fecal samples showed increased expression of the L-arabinose catabolism pathway in vivo. By combining bacterial genetics and diet manipulation, we demonstrate that diet-derived L-arabinose provides S. Tm a competitive advantage in the gut. We show that expansion of S. Tm in the gut requires a previously uncharacterized alpha-N-arabinofuranosidase that can liberate L-arabinose from dietary polysaccharides. Ultimately, we demonstrate that pathogen-liberated L-arabinose from the diet provides a competitive advantage to S. Tm in vivo. These findings propose L-arabinose as a critical driver of S. Tm expansion in the guts of superspreader hosts.

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“…A subsequent study characterised a GH76 enzyme from a marine bacterial species of Salegentibacter showed endo α1,6-mannanase activity against yeast α-mannan, which suggests that fungi in aquatic environments also produce α-mannan for their cell walls [ 31 ]. The gene for this GH76 is in an operon with CAZymes from families GH92, GH2, GH125, and GH43, so it is likely this bacterium is equipped to breakdown complex fungal α-mannan structures.…”

Section: Deconstruction Of Fungal α-Mannanmentioning

confidence: 99%

N-glycan breakdown by bacterial CAZymes

Crouch

2023

Essays in Biochemistry

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The modification of proteins by N-glycans is ubiquitous to most organisms and they have multiple biological functions, including protecting the adjoining protein from degradation and facilitating communication or adhesion between cells, for example. Microbes have evolved CAZymes to deconstruct different types of N-glycans and some of these have been characterised from microbes originating from different niches, both commensals and pathogens. The specificity of these CAZymes provides clues as to how different microbes breakdown these substrates and possibly cross-feed them. Discovery of CAZymes highly specific for N-glycans also provides new tools and options for modifying glycoproteins.

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Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi

Cited by 8 publications

References 99 publications

Abundance and composition of particles and their attached microbiomes along an Atlantic Meridional Transect

Abundance and composition of particles and their attached microbiomes along an Atlantic Meridional Transect

Multi-omics reveal Salmonella-liberated dietary L-arabinose promotes expansion in superspreaders

N-glycan breakdown by bacterial CAZymes

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