Glycosidases in the Nervous System

Robins, Eli; Hirsch, Hilde E.; Emmons, Susan

doi:10.1016/s0021-9258(18)93249-7

Cited by 104 publications

(1 citation statement)

References 31 publications

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“…The activities of the following enzymes were determined as markers for respective subcellular organelles: 5'-nucleotidase for plasma membranes (Bartlett, 1959;Morre, 1971); glucose 6-phosphatase for microsomal fraction (Bartlett, 1959;Morre, 1971); succinate-p-iodonitrotetrazolium Violet reductase for mitochondria (Morr6, 1971); arylsulphatase (Sherman & Stanfield, 1967), fi-N-acetylglucosaminidase (Bowers et al, 1967), 6-galactosidase (Robins et al, 1968), acid phosphatase (Bowers et al, 1967), f,-glucuronidase (Allison & Sandelin, 1963;Robins et al, 1968) and cathepsin D (method III of Barrett, 1972) for lysosomes.…”

Section: Methodsmentioning

confidence: 99%

Heterogeneity of lysosomes originating from rat liver parenchymal cells. Metabolic relationship of subpopulations separated by density-gradient centrifugation

Pertoft

Wärmegård

Höök

1978

Biochemical Journal

117

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1. A crude lysosomal fraction obtained by differential centrifugation of a rat liver homogenate was subjected to zonal centrifugation in iso-osmotic self-generating gradients composed of modified colloidal silica (Percoll). Analysis of relevant marker-enzyme activities shows a continuous band of considerably purified lysosomal particles in the density range 1.04--1.11 g/ml. 2. A relationship between age and buoyant density of the parenchymal lysosomal subpopulations is indicated by the distribution of 125I-labelled asialoglycoproteins in the heterogeneous lysosomes during the catabolism of the glycoprotein. The labelled asialoglycoprotein first appeared in lysosomal particles of low density, which with time progressively acquired a higher density. Furthermore, 30 min after administration the 125I-labelled asialocaeruloplasmin recovered in the light lysosomes was less degraded than the material recovered in the heavy lysosomes. 3. A lysosomal enzyme (arylsulphatase) was found to possess considerably higher isoelectric points in the heavy lysosomes than in the light lysosomes, which is consistent with a relationship between age and density of the lysosomes.

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Section: Methodsmentioning

confidence: 99%

Heterogeneity of lysosomes originating from rat liver parenchymal cells. Metabolic relationship of subpopulations separated by density-gradient centrifugation

Pertoft

Wärmegård

Höök

1978

Biochemical Journal

117

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Low molecular weight inhibitors of matrix metalloproteinases can enhance the expression of matrix metalloproteinase‐2 (gelatinase A) without inhibiting its activation

Kerkvliet

Jansen

Schoenmaker

et al. 2003

Cancer

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BACKGROUNDIn the current study, the authors investigated the effects of synthetic low molecular weight inhibitors of matrix metalloproteinases (MMPs) on the expression and activation of MMP‐2 in a three‐dimensional tissue system.METHODSRabbit periosteal explants were cultured with or without various concentrations of the MMP inhibitors CT1166, CT1399, or CT1746, and conditioned media and tissue extracts were analyzed for the expression and activity of MMP‐2.RESULTSThe data showed that blocking the activity of all MMPs with relatively high inhibitor concentrations completely prevented the conversion of pro‐MMP‐2 into its active form and that the level of protein was decreased. Selective inhibition of the activity of gelatinases (MMP‐2 and MMP‐9) by using low inhibitor concentrations, however, induced a higher level of active MMP‐2 and increased its expression significantly.CONCLUSIONSThe current observations indicate that selective inhibitors of MMPs affect the expression and activity of MMP‐2, thus providing clues regarding the differing effects such inhibitors appear to have when applied in vivo. Cancer 2003;97:1582–88. © 2003 American Cancer Society.DOI 10.1002/cncr.11193

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Appearance of cytolytic granules upon induction of cytolytic activity in CTL-hybrids.

Masson¹,

Corthésy²,

Nabholz³

et al. 1985

The EMBO Journal

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Examining a CTL‐line (B6.1) with specific cytolytic activity, we have confirmed the ability of CTL‐lines to produce poly C9‐like, tubular complexes of two sizes (polyperforin 1 and 2). Isolated cytoplasmic granules from this cell line can induce hemolysis and contain the lysosomal marker arylsulfatase. Electron microscopial studies show that granules contain precursor molecules which are assembled into polyperforin upon addition of Ca2+. Antibodies raised against granules predominantly react with a 27‐kd protein. We compared the B6.1 cell with a hybrid, PC60, derived from a cross between B6.1 and a non‐cytolytic rat‐thymoma. This hybrid line exhibits inducible CTL‐activity. In PC60 with induced cytolytic activity, polyperforins 1 and 2, cytolytic granules and granule‐associated proteins are detected. Non‐cytolytic PC60 cells lack cytolytic granules and polyperforin 1, but contain polyperforin 2. These results suggest that cytolytic granules appear in parallel with the expression of cytotoxicity during T‐lymphocyte differentiation.

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Glycosidases in the Nervous System

Cited by 104 publications

References 31 publications

Heterogeneity of lysosomes originating from rat liver parenchymal cells. Metabolic relationship of subpopulations separated by density-gradient centrifugation

Heterogeneity of lysosomes originating from rat liver parenchymal cells. Metabolic relationship of subpopulations separated by density-gradient centrifugation

Low molecular weight inhibitors of matrix metalloproteinases can enhance the expression of matrix metalloproteinase‐2 (gelatinase A) without inhibiting its activation

Appearance of cytolytic granules upon induction of cytolytic activity in CTL-hybrids.

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