Glycosidase determination in bovine oviducal fluid at the follicular and luteal phases of the oestrous cycle

Carrasco, L.; Coy, Pilar; Sánchez, Manuel A. Bueno; Gadea, J.; Romar, Raquel

doi:10.1071/rd08113

Cited by 39 publications

(22 citation statements)

References 61 publications

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“…The latter has been identified in the porcine oviduct mainly during oestrus (Buhi & Alvarez, 2003). Other molecules of interest are glycodelins (Chiu et al , 2007; Yeung et al , 2007), retinol‐binding protein, haemoglobin beta chain, Ig kappa light chain and variable region, fibrinogen A‐alpha‐chain, fibrinogen beta chain precursor, (Georgiou et al , 2007) different sulphated and non‐sulphated glycosaminoglycans (Tienthai et al , 2000; Bergqvist & Rodriguez‐Martinez, 2006; Liberda et al , 2006), and glycosidases (Carrasco et al , 2008 a , b ). The inclusion of recombinant human albumin and hyaluronan in current human IVF media provides a precedent for the use of recombinant OVGP1, C3 or different S‐GAGs in future culture media.…”

Section: Discussionmentioning

confidence: 99%

What controls polyspermy in mammals, the oviduct or the oocyte?

Coy

Sánchez

2010

Biological Reviews

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A block to polyspermy is required for successful fertilisation and embryo survival in mammals. A higher incidence of polyspermy is observed during in vitro fertilisation (IVF) compared with the in vivo situation in several species. Two groups of mechanisms have traditionally been proposed as contributing to the block to polyspermy in mammals: oviduct-based mechanisms, avoiding a massive arrival of spermatozoa in the proximity of the oocyte, and egg-based mechanisms, including changes in the membrane and zona pellucida (ZP) in reaction to the fertilising sperm. Additionally, a mechanism has been described recently which involves modifications of the ZP in the oviduct before the oocyte interacts with spermatozoa, termed "pre-fertilisation zona pellucida hardening". This mechanism is mediated by the oviductal-specific glycoprotein (OVGP1) secreted by the oviductal epithelial cells around the time of ovulation, and is reinforced by heparin-like glycosaminoglycans (S-GAGs) present in oviductal fluid. Identification of the molecules contributing to the ZP modifications in the oviduct will improve our knowledge of the mechanisms of sperm-egg interaction and could help to increase the success of IVF systems in domestic animals and humans.

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Section: Discussionmentioning

confidence: 99%

What controls polyspermy in mammals, the oviduct or the oocyte?

Coy

Sánchez

2010

Biological Reviews

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“…Once dissected, the oviducts were not opened lengthwise but the porcine OF from whole oviduct was collected by aspiration with an automatic pipette by introducing the tip into the ampulla for a maximum 200 ml volume and making a manual ascendent pressure from the isthmus to the ampulla as described previously (Carrasco et al 2008). Once aspirated, the OF was centrifuged at 7000 g for 10 min at 4 8C to remove cellular debris.…”

Section: Oviducts Classification and Collection Of Ofmentioning

confidence: 99%

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Carrasco

Romar

Sánchez³

et al. 2008

Reproduction

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Sperm-oocyte binding and gamete-oviductal epithelium interactions are carbohydrate-mediated events occurring in the oviductal fluid (OF). Thus, knowledge about the activities of glycosidases (enzymes catalyzing hydrolytic cleavage of terminal sugar residues) in this milieu would help us understand the molecular mechanisms involved in these events. This work was carried out to investigate the glycosidase activity, protein content, and volume of OF collected from gilts and sows. Oviducts were classified into four phases of the estrous cycle (early follicular, late follicular, early luteal, and late luteal) based on the appearance of the ovaries. OF was aspirated, centrifuged, measured for volume, and frozen until assay. Substrates conjugated to 4-methylumbelliferyl were used to screen the activities of seven different glycosidases at physiological pH (7.2). a-L-Fucosidase and b-N-acetyl-glucosaminidase activities increased at the late follicular phase to decrease after ovulation. b-D-Galactosidase, a-D-mannosidase, and b-N-acetyl-galactosaminidase showed higher activities at the early follicular phase, which decreased after ovulation. N-Acetyl-neuraminidase and a-D-galactosidase did not show activity at any phase of estrous cycle neither in sows nor in gilts at pH 7.2, although it did at acidic pH (4.4) in the follicular and luteal phase samples. Total protein also changed during the cycle showing the maximum secretion at the late follicular phase (2118.6G200.7 mg/oviduct). The highest volumes of OF were collected from the oviducts at the late follicular phase (50.7G1.3 ml/oviduct). These results indicate that OF from sows and gilts shows glycosidase activity varying throughout the estrous cycle suggesting a role of these enzymes in carbohydrate-mediated events. Reproduction (2008) 136 833-842

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“…These enzymes have been detected in hamster, porcine, and bovine oviductal secretion (Tulsiani et al 1996, Carrasco et al 2008a. It has been suggested that these enzymes could affect the protein and carbohydrate distribution on the sperm and the ZP surface and could modulate sperm-oocyte binding and gamete-oviductal epithelium interaction, both suggested to be carbohydratemediated events occurring in the presence of the oviductal fluid (Carrasco et al 2008a, b).…”

Section: Glycosidase Enzymesmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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Glycosidase determination in bovine oviducal fluid at the follicular and luteal phases of the oestrous cycle

Cited by 39 publications

References 61 publications

What controls polyspermy in mammals, the oviduct or the oocyte?

What controls polyspermy in mammals, the oviduct or the oocyte?

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Oviductal secretion and gamete interaction

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