Glycosaminoglycans in the aorta of six animal species

Engel, Ulrike

doi:10.1016/0021-9150(71)90005-0

Cited by 39 publications

(9 citation statements)

References 39 publications

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“…DS contributes a large part of the GAGs [28], Corre spondingly. our finding that CS and DS constitute most of the GAGs synthesized by the SMCs is in accord with other studies [13,[26][27][28], Moreover, the increase in the content of CS with a concomitant decrease in DS content is also similar to the reports describing injury-induced changes in intimai-medial PGs and GAGs [4,7,13], The changes in CS proportions are found to be correlated with increased atherogeneitv. because of the greater af finity of CS-rich PGs for lipoproteins [29][30][31], but the effect of DS on SMC growth and function is still unclear, though one report suggests that PGs may facilitate cell division [24], In conclusion, the data presented show that de-endothelialization of rabbit aortas not only augmented PG synthesis by SMCs in culture but also altered the GAG distribution of PGs.…”

Section: Resultssupporting

confidence: 82%

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Proteoglycan Synthesis by the Neointimal Smooth Muscle Cells Cultured from Rabbit Aortic Explants following De-Endothelialization

Li¹,

Alavi²,

Wasty³

et al. 1993

Pathobiology

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Proteoglycans (PGs), the essential component of the extracellular matrix, are implicated in the pathogenesis of atherosclerosis. In an experimental model of injury, PGs accumulate in the neointimal tissue parallel with lipid deposition. However, it is still not clear whether the PG accumulation is from active smooth muscle cell (SMC) production or is a consequence of trapping within neointima covered by endothelium. To study the effect of endothelial injury on PG synthesis, SMCs were cultured from normal aorta (N-SMC), neointima covered by regenerated endothelium (W-SMC) and neointima without endothelium (B-SMC). Using [³⁵S]-Na2SC·4, as a precursor in an in vitro incubation, the kinetics of PG synthesis were determined. PG synthesis by all three cell types increases as a function of time. It is significantly higher in the SMCs cultured from endothelium-denuded aortic explants (W- and B-SMC) than N-SMC. This finding indicates that endothelial injury stimulates PG synthesis by SMCs.

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Section: Resultssupporting

confidence: 82%

“…The proportion of HS was almost the same in all SMC types. It is well known that GAG distribution is species specific [26][27], In rab- bit aorta, CS is dominant [27] whereas, in cultured SMC. DS contributes a large part of the GAGs [28], Corre spondingly.…”

Section: Resultsmentioning

confidence: 99%

Proteoglycan Synthesis by the Neointimal Smooth Muscle Cells Cultured from Rabbit Aortic Explants following De-Endothelialization

Li¹,

Alavi²,

Wasty³

et al. 1993

Pathobiology

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“…3), and the relative levels of binding in thoracic and abdominal aorta, are qualitatively comparable with these variations in human GAGs. It may be noted, however, that in the rat and certain other non-human species Engel (1971) failed to confirm increased sulphated GAGs in abdominal aorta. Nor do changes in GAG levels readily explain the fall in the bound Na+ fraction between 1 and 3 months.…”

Section: Discussionmentioning

confidence: 99%

“…This possibility cannot be rigidly excluded in the present study. Among connective tissue components GAGs are those whose exclusive properties have been most widely studied (for review see Laurent, 1970), but they form only a very small fraction of total aortic macromolecules in the rat (Engel, 1971). From data on the ratio of distribution of high-molecular-weight polyethylene glycol to mannitol in a variety of tissues, relative to tissue GAG content, Comper & Laurent (1978) concluded that in aortic wall it is elastin and collagen which are chiefly responsible for solute exclusion.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Age on Fluid and Sodium Chloride Distribution in Rat Aortic Wall

Law

1984

Exp Physiol

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SUMMARYFluid, Na+ and Cl-content and distribution have been examined in incubated segments of thoracic and abdominal aorta from male Wistar rats aged 1, 3 and 18 months. Na+ and Clwere determined under conditions of total metabolic blockade (cyanide+iodoacetate). The total aortic mural fluid content, relative to solute-free dry weight, fell from I to 3 months, due mainly to relative contraction of the extracellular (e.c.) (inulin) space. It increased from 3 to 18 months due mainly to an increased intracellular (i.c.) (non-inulin) space. Total mural free and bound Na+ content, as well as i.c. Na+ content and concentration, fell from 1 to 3 months and increased from 3 to 18 months. No bound Na+ was detectable in segments which had been incubated in the presence of chondroitinase ABC. I.c. Cl-content and concentration fell from 1 to 3 months, but thereafter did not alter significantly. Bound Cl-in the aortic wall of 3-month-old rats was markedly reduced by incubation in the presence of a non-specific protease preceded by treatment with glycerol. The results are discussed in relation to previously established age-related characteristics of aortic and arterial walls.

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“…Les résultats concernant les GAG de la paroi artérielle (aorte abdominale) complètent l'étude faite précédemment sur l'aorte thoracique (24). Comme l'avait observé Engle (26), on note une diffé rence de distribution des GAG entre les fragments thoracique et abdominal de l'aorte.…”

Section: Distribution Des Gag Dans Différents Tissus Chez Le Ratunclassified

Influence de la vitamine A sur la distribution des glycosaminoglycanes dans les tissus du rat

Lacord-Bonneau¹,

Picard²,

Dubernard³

1975

Ann Nutr Metab

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Glycosaminogly cans (GAG) from brains, intestines, livers and skin of rats were analyzed by electrophoretic and enzymatic methods. Variations in GAG distribution have been studied in rats fed on a vitamin-A-deficient diet and on a diet supplemented with an excessive quantity of vitamin A. Hypervitaminosis A resulted in a decrease in GAG isolated from connective and hepatic tissues. The incorporation of labeled sulfate into the GAG of these tissues has been studied. Hypervitaminosis A resulted in a decrease in sulfate incorporation into the GAG isolated from connective tissues. A large increase in sulfate incorporation into the GAG of the digestive tract is observed for vitamin-A-treated rats. A decrease is noted for vitamin-A-deficient rats. It was suggested that these variations in distribution and specific activity of GAG could be due to different effects of vitamin A. Vitamin A seems to be required either for the release of lysosomal enzymes or for the sulfatation and elongation of certain glucidic chains in the biosynthesis of sulfated GAG.

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Glycosaminoglycans in the aorta of six animal species

Cited by 39 publications

References 39 publications

Proteoglycan Synthesis by the Neointimal Smooth Muscle Cells Cultured from Rabbit Aortic Explants following De-Endothelialization

Proteoglycan Synthesis by the Neointimal Smooth Muscle Cells Cultured from Rabbit Aortic Explants following De-Endothelialization

The Influence of Age on Fluid and Sodium Chloride Distribution in Rat Aortic Wall

Influence de la vitamine A sur la distribution des glycosaminoglycanes dans les tissus du rat

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