Glycosaminoglycan mimetics trigger IP3-dependent intracellular calcium release in myoblasts

Martelly, Isabelle; Singabraya, Dominique; Vandebrouck, Aurélie; Papy-García, Dulce; Cognard, Christian; Raymond, Guy; Guillet-Deniau, Isabelle; Courty, José; Constantin, Bruno

doi:10.1016/j.matbio.2010.02.002

Cited by 7 publications

(4 citation statements)

References 50 publications

(56 reference statements)

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“… 6 In addition, heparin activates the serotonin receptor and serotonin signaling pathways. 7 Versican, a cell membrane proteoglycan, promotes the sensitization of mechanosensitive currents in nociceptive sensory neurons. 8 The interaction between toll-like receptors and biglycan, a leucine-rich repeat proteoglycan, has also been described on macrophages.…”

Section: Introductionmentioning

confidence: 99%

“… 20 Post-translational mechanisms to regulation P2X receptor activity have also been described, such as phosphoinositides, 21 phosphorylation 22 and integrins. 23 , 24 Although previous studies have shown that P2X receptors are associated with the regulation of cellular biosynthesis and secretion of proteoglycans, 7 the influence of GAGs on the physiology of these receptor classes has not been fully evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Post-translational allosteric activation of the P2X7 receptor through glycosaminoglycan chains of CD44 proteoglycans

Moura

Lucena

Lima

et al. 2015

Cell Death Discovery

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Here, we present evidence for the positive allosteric modulation of the P2X7 receptor through glycosaminoglycans (GAGs) in CHO (cell line derived from the ovary of the Chinese hamster) cells. The marked potentiation of P2X7 activity through GAGs in the presence of non-saturating agonists concentrations was evident with the endogenous expression of the receptor in CHO cells. The presence of GAGs on the surface of CHO cells greatly increased the sensitivity to adenosine 5′-triphosphate and changed the main P2X7 receptor kinetic parameters EC50, Hill coefficient and Emax. GAGs decreased the allosteric inhibition of P2X7 receptor through Mg2+. GAGs activated P2X7 receptor-mediated cytoplasmic Ca2+ influx and pore formation. Consequently, wild-type CHO-K1 cells were 2.5-fold more sensitive to cell death induced through P2X7 agonists than mutant CHO-745 cells defective in GAGs biosynthesis. In the present study, we provide the first evidence that the P2X7 receptor interacts with CD44 on the CHO-K1 cell surface. Thus, these data demonstrated that GAGs positively modulate the P2X7 receptor, and sCD44 is a part of a regulatory positive feedback loop linking P2X7 receptor activation for the intracellular response mediated through P2X7 receptor stimulation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Post-translational allosteric activation of the P2X7 receptor through glycosaminoglycan chains of CD44 proteoglycans

Moura

Lucena

Lima

et al. 2015

Cell Death Discovery

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“…Increase in calcium concentrations might be another possible explanation to why GAGs improve bovine cell myoblasts proliferation and differentiation. Artificial GAGs applied to pre-fusing myoblasts induced transient increase in calcium concentrations, and any mechanism that increases intracellular calcium is expected to trigger myoblast differentiation (Martelly et al, 2010). An interesting observation in our study is that neither GAGs alone, nor ECL alone, did influence early myogenesis significantly.…”

Section: Discussionmentioning

confidence: 99%

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

et al. 2013

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Primary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation and differentiation features, were characterized using immunocytochemistry, western blotting and real-time PCR. We observed a reorganization of the Golgi complex in differentiated cells. The Golgi complex transformed to a highly fragmented network of small stacks of cisternae positioned throughout the myotubes as well as around the nucleus. Different extracellular matrix (ECM) components were used as surface coatings in order to improve cell culture conditions. Our experiments demonstrated improved proliferation and early differentiation for cells grown on surface coatings containing a mixture of both glycosaminoglycans (GAGs) and fibrous proteins. We suggest that GAGs and fibrous proteins mixed together into a composite biomaterial can mimic a natural ECM, and this could improve myogenesis for in vitroin vitro cell cultures.Abbreviations: used: ECMextracellular (The author would like to point out that there should be a space between the abbrevations and the subsequent explanation. ) matrix; GAGsglucosaminoglycans;MRFGAGsglucosaminoglycans, MRF, myogenic regulatory transcription factor; PGproteoglycan

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“…16 The fluorescein-labeled derivative [OTR 4120 ] FITC was prepared from dextran-FITC (Sigma) as previously described. 29 Heparin from porcine intestinal mucosa (Sigma) and HS from the bovine kidney (Sigma) were used as natural GAG controls and were cell culture grade. Recombinant human FGF-2 was prepared as previously described.…”

Section: Methodsmentioning

confidence: 99%

Glycosaminoglycan Mimetic Associated to Human Mesenchymal Stem Cell-Based Scaffolds Inhibit Ectopic Bone Formation, but Induce Angiogenesis In Vivo

Frescaline

Bouderlique

Mansoor

et al. 2013

Tissue Engineering Part A

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Tissue engineering approaches to stimulate bone formation currently combine bioactive scaffolds with osteocompetent human mesenchymal stem cells (hMSC). Moreover, osteogenic and angiogenic factors are required to promote differentiation and survival of hMSC through improved vascularization through the damaged extracellular matrix (ECM). Glycosaminoglycans (GAGs) are ECM compounds acting as modulators of heparinbinding protein activities during bone development and regenerative processes. GAG mimetics have been proposed as ECM stabilizers and were previously described for their positive effects on bone formation and angiogenesis after local treatment. Here, we developed a strategy associating the GAG mimetic [OTR 4120 ] with bone substitutes to optimize stem cell-based therapeutic products. We showed that [OTR 4120 ] was able to potentiate proliferation, migration, and osteogenic differentiation of hMSC in vitro. Its link to tricalcium phosphate/hydroxyapatite scaffolds improved their colonization by hMSC. Surprisingly, when these combinations were tested in an ectopic model of bone formation in immunodeficient mice, the GAG mimetics inhibit bone formation induced by hMSC and promoted an osteoclastic activity. Moreover, the inflammatory response was modulated, and the peri-implant vascularization stimulated. All together, these findings further support the ability of GAG mimetics to organize the local ECM to coordinate the host response toward the implanted biomaterial, and to inhibit the abnormal bone formation process on a subcutaneous ectopic site.

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Glycosaminoglycan mimetics trigger IP3-dependent intracellular calcium release in myoblasts

Cited by 7 publications

References 50 publications

Post-translational allosteric activation of the P2X7 receptor through glycosaminoglycan chains of CD44 proteoglycans

Post-translational allosteric activation of the P2X7 receptor through glycosaminoglycan chains of CD44 proteoglycans

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

Glycosaminoglycan Mimetic Associated to Human Mesenchymal Stem Cell-Based Scaffolds Inhibit Ectopic Bone Formation, but Induce Angiogenesis In Vivo

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