Glycosaminoglycan from <i>Ostrea rivularis</i> attenuates hyperlipidemia and regulates gut microbiota in high‐cholesterol diet‐fed zebrafish

Kong, Yan; Liu, Ying; Dai, Zhicheng; Qin, Mei; Fan, Heliang; Hao, Junguang; Zhang, Chenxiao; Zhong, Qiuping; Cen, Qi; Wang, Pei

doi:10.1002/fsn3.2492

Cited by 11 publications

(9 citation statements)

References 57 publications

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“…Our results show that administering a high-fat diet (8% cholesterol) to adult zebrafish and zebrafish larvae resulted in an excess accumulation of triglycerides and cholesterol within their bodies (Figure 1). This aligns with the outcomes of a high-fat zebrafish model developed by Yan Kong, which involved feeding zebrafish a diet containing 4% cholesterol [50]. After 5 days of consumption of β-sitosterol using the aforementioned model, the accumulation of triglycerides and cholesterol in zebrafish was decreased, which matched with the lipid-lowering properties of the drug bezafibrate (Figure 1).…”

Section: Discussionsupporting

confidence: 72%

β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model

Zhang,

Liu,

Xue

et al. 2024

Animals

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Objective: Non-alcoholic fatty liver disease (NAFLD) is strongly associated with hyperlipidemia, which is closely related to high levels of sugar and fat. β-sitosterol is a natural product with significant hypolipidemic and cholesterol-lowering effects. However, the underlying mechanism of its action on aquatic products is not completely understood. Methods: A high-fat diet (HFD)-induced NAFLD zebrafish model was successfully established, and the anti-hyperlipidemic effect and potential mechanism of β-sitosterol were studied using oil red O staining, filipin staining, and lipid metabolomics. Results: β-sitosterol significantly reduced the accumulation of triglyceride, glucose, and cholesterol in the zebrafish model. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differential lipid molecules in β-sitosterol mainly regulated the lipid metabolism and signal transduction function of the zebrafish model. β-sitosterol mainly affected steroid biosynthesis and steroid hormone biosynthesis in the zebrafish model. Compared with the HFD group, the addition of 500 mg/100 g of β-sitosterol significantly inhibited the expression of Ppar-γ and Rxr-α in the zebrafish model by at least 50% and 25%, respectively. Conclusions: β-sitosterol can reduce lipid accumulation in the zebrafish model of NAFLD by regulating lipid metabolism and signal transduction and inhibiting adipogenesis and lipid storage.

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Section: Discussionsupporting

confidence: 72%

β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model

Zhang,

Liu,

Xue

et al. 2024

Animals

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“…Here, we show that Proteobacteria abundance correlated positively with the intake of dietary cholesterol and cheese. A high-cholesterol diet was recently shown to increase Proteobacteria abundance in zebrafish ( 55 ), and high-fat feeding resulted in higher abundance of Proteobacteria in mice ( 56 – 58 ). Furthermore, Proteobacteria has been reported to increase in low-grade inflammation, a common feature of metabolic diseases ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota is associated with dietary intake and metabolic markers in healthy individuals

Gaundal

Myhrstad

Rud

et al. 2022

Food & Nutrition Research

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Background: Metabolic diseases have been related to gut microbiota, and new knowledge indicates that diet impacts host metabolism through the gut microbiota. Identifying specific gut bacteria associated with both diet and metabolic risk markers may be a potential strategy for future dietary disease prevention. However, studies investigating the association between the gut microbiota, diet, and metabolic markers in healthy individuals are scarce. Objective: We explored the relationship between a panel of gut bacteria, dietary intake, and metabolic and anthropometric markers in healthy adults. Design: Forty-nine volunteers were included in this cross-sectional study. Measures of glucose, serum triglyceride, total cholesterol, hemoglobin A1c (HbA1c), blood pressure (BP), and body mass index (BMI) were collected after an overnight fast, in addition to fecal samples for gut microbiota analyzes using a targeted approach with a panel of 48 bacterial DNA probes and assessment of dietary intake by a Food Frequency Questionnaire (FFQ). Correlations between gut bacteria, dietary intake, and metabolic and anthropometric markers were assessed by Pearson's correlation. Gut bacteria varying according to dietary intake and metabolic markers were assessed by a linear regression model and adjusted for age, sex, and BMI. Results: Of the 48 gut bacteria measured, 24 and 16 bacteria correlated significantly with dietary intake and metabolic and/or anthropometric markers, respectively. Gut bacteria including Alistipes, Lactobacillus spp., and Bacteroides stercoris differed according to the intake of the food components, fiber, sodium, saturated fatty acids, and dietary indices, and metabolic markers (BP and total cholesterol) after adjustments. Notably, Bacteroides stercoris correlated positively with the intake of fiber, grain products, and vegetables, and higher Bacteroides stercoris abundance was associated with higher adherence to Healthy Nordic Food Index (HNFI) and lower diastolic BP after adjustment. Conclusion: Our findings highlight the relationship between the gut microbiota, diet, and metabolic markers in healthy individuals. Further investigations are needed to address whether these findings are causally linked and whether targeting these gut bacteria can prevent metabolic diseases.

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Section: Hfd-induced Hyperlipidemiamentioning

confidence: 99%

“…Natural polysaccharides isolated from Cordyceps militaris , alleviate hyperglycemia in high-fat/sucrose diet-fed mice by improving gut microbiota dysbiosis, and promoting the abundance of probiotics, Akkermansia muciniphila ( Lee et al, 2021 ). Natural polysaccharides isolated from seafood Ostrea rivularis , attenuate hyperlipidemia in HFD-fed zebrafish by improving gut microbiota imbalance, notably by enriching the beneficial gut bacteria, Acidobacteria , Bacteroidetes , and Verrucomicrobia , and reducing the harmful bacteria, Proteobacteria and Cohaesibacter ( Kong et al, 2021 ). Selenium-rich polysaccharides extracted from Cordyceps militaris prevent HFD-induced hyperlipidemia in mice by reducing obesity-correlated gut bacteria, including Dorea , Clostridium , Lactobacillus , and Ruminococcus , and increasing the beneficial gut bacteria, Akkermansia ( Yu et al, 2021 ).…”

Section: Hfd-induced Hyperlipidemiamentioning

confidence: 99%

Targeting Gut Microbiota With Natural Polysaccharides: Effective Interventions Against High-Fat Diet-Induced Metabolic Diseases

et al. 2022

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Unhealthy diet, in particular high-fat diet (HFD) intake, can cause the development of several metabolic disorders, including obesity, hyperlipidemia, type 2 diabetes mellitus (T2DM), non-alcoholic fatty liver disease (NAFLD), and metabolic syndrome (MetS). These popular metabolic diseases reduce the quality of life, and induce premature death worldwide. Evidence is accumulating that the gut microbiota is inextricably associated with HFD-induced metabolic disorders, and dietary intervention of gut microbiota is an effective therapeutic strategy for these metabolic dysfunctions. Polysaccharides are polymeric carbohydrate macromolecules and sources of fermentable dietary fiber that exhibit biological activities in the prevention and treatment of HFD-induced metabolic diseases. Of note, natural polysaccharides are among the most potent modulators of the gut microbiota composition. However, the prebiotics-like effects of polysaccharides in treating HFD-induced metabolic diseases remain elusive. In this review, we introduce the critical role of gut microbiota human health and HFD-induced metabolic disorders. Importantly, we review current knowledge about the role of natural polysaccharides in improving HFD-induced metabolic diseases by regulating gut microbiota.

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Glycosaminoglycan from Ostrea rivularis attenuates hyperlipidemia and regulates gut microbiota in high‐cholesterol diet‐fed zebrafish

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 57 publications

β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model

β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model

Gut microbiota is associated with dietary intake and metabolic markers in healthy individuals

Targeting Gut Microbiota With Natural Polysaccharides: Effective Interventions Against High-Fat Diet-Induced Metabolic Diseases

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